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Volume 20, Number 1—January 2014
Dispatch

Increasing Threat of Brucellosis to Low-Risk Persons in Urban Settings, China

Shouyi Chen1, Hao Zhang1, Xiaoning Liu1, Wenjing Wang1, Shuiping Hou, Tingting Li, Shuoxian Zhao, Zhicong Yang, and Chengyao LiComments to Author 
Author affiliations: Center for Disease Control and Prevention, Guangzhou, China (S. Chen, H. Zhang, X. Liu, S. Hou, Z. Yang); Southern Medical University, Guangzhou, China (W. Wang, T. Li, S. Zhao, C. Li)

Main Article

Figure 1

Detection and identification of Brucella DNA. A) Detection of Brucella DNA by quantitative PCR. Numbers indicate amplification curves with cycle threshold (Ct) values representative of samples. Curve 1, sample from patient 1 with 16 Ct value; curve 2, sample from patient 2 with 16 Ct; curve 3, sample from patient 3 with 17 Ct; curve 4, stem cells of cord blood from patient 1 with 34 Ct; curve 5, stem cells of cord blood from patient 2 with 34.5 Ct; curve 6, positive control with 18 Ct, curve 7,

Figure 1. Detection and identification of Brucella DNA. A) Detection of Brucella DNA by quantitative PCR. Numbers indicate amplification curves with cycle threshold (Ct) values representative of samples. Curve 1, sample from patient 1 with 16 Ct value; curve 2, sample from patient 2 with 16 Ct; curve 3, sample from patient 3 with 17 Ct; curve 4, stem cells of cord blood from patient 1 with 34 Ct; curve 5, stem cells of cord blood from patient 2 with 34.5 Ct; curve 6, positive control with 18 Ct, curve 7, negative control with no Ct. B) Amplification of Brucella DNA by AMOS-PCR. Numbers indicate the amplified DNA bands representative of samples. Lane 1, DNA molecular weight marker, values along the left side are base pairs; lane 2, sample from patient 1; lane 3, sample from patient 2; lane 4, sample from patient 3; lane 5, stem cells from cord blood of patient 1; lane 6, stem cells from cord blood of patient 2; lane 7, negative control.

Main Article

1These authors contributed equally to this article.

Page created: December 18, 2013
Page updated: December 18, 2013
Page reviewed: December 18, 2013
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