Volume 20, Number 2—February 2014
Poxvirus Viability and Signatures in Historical Relics
|Location, date of origination, description of the artifact (date discovered)||Laboratory testing*
|Live virus isolated||Evidence by electron microscopy||Viral DNA isolated||Human DNA isolated||Other testing|
|Egypt, 1157 bce, mummy of Ramses V with lesions; lesions were present in a centrifugal distribution and had an appearance similar to smallpox (1898, 1979)||No (2)||No (2)||No†||No†||Viral particles and faint immunologic reactivity with variola antibody; negative radioimmunoassay result for smallpox (23)|
|Egypt, 1200–1100 bce, piece of skin from male mummy with a typical smallpox rash (1911)||Portion of skin did not show definite pathologic characteristics of smallpox (24)|
|Italy, sixteenth century, corpse exhumed from a crypt; lesions were umbilicated, monomorphic, and in a centrifugal distribution (1986)||No (25)||Yes (25,26)||No, by molecular hybridization (29); no, by DNA isolation and real-time PCR†||No†||Orthopoxvirus antigens not detected by hemagglutination or enzyme immunoassay (25)|
|Canada, 1640–1650, bones from an adult man located in a burial plot on Native American land; the tribe was known to have had a smallpox epidemic in 1634 (1966)||Bone analysis result was consistent with osteomyelitis variolosa (27)|
|Russia, late seventeenth to early eighteenth centuries, corpses exhumed from permafrost; 1 grave had multiple bodies and evidence suggested quick postmortem burial; samples were analyzed from 1 corpse (2004)||Yes, variola virus–related DNA (28)|
|England, 1729–1856, piece of skin with lesions attached to a skeleton exhumed from a crypt (1985)||No (29)||No†||No†|
|Russia, nineteenth century, corpses in permafrost recovered during flooding; corpses were from an area of a smallpox outbreak in the nineteenth century (1991)||No (30)|
|Kentucky, USA, 1840–1860, mummified remains of a body with lesions discovered at a construction site (2000)||No†||No†*|
|New York, New York, USA, City, mid-1800s, mummified remains of a body with lesions contained within an iron coffin discovered at a construction site (2011)||No†||No†||No†||Yes, from a tooth†|
|Virginia, USA, 1876, scab from the arm of an infant to be used for community vaccination; found in letter sent from son to father in Virginia; scab was on display at a museum (2011)||No†||Yes, non-variola Orthopoxvirus DNA†||Yes†|
|New Mexico, USA, late nineteenth century, scabs from vaccination sites contained in an envelope, which was contained within a book (2003)||No†||Yes, non-variola Orthopoxvirus DNA†||No†|
|Arkansas, USA, 1871–1926, suspected smallpox scabs on display at a museum (2004)||No†||No†||No†|
*Published laboratory results are accompanied by the reference (number in parentheses).
†Previously unpublished results.
- Fenner F, Henderson DA, Arita I, Jezek Z, Ladnyi ID. Smallpox and its eradication. Geneva: World Health Organization; 1988.
- Hopkins DR. Ramses V. Earliest known victim? World Health. 1980, May 22 [cited 2013 Dec 2]. http://whqlibdoc.who.int/smallpox/WH_1980.pdf
- Deria A, Jezek Z, Markvart K, Carrasco P, Weisfeld J. The world’s last endemic case of smallpox: surveillance and containment measures. Bull World Health Organ. 1980;58:279–83 .
- Breman JG, Henderson DA. Diagnosis and management of smallpox. N Engl J Med. 2002;346:1300–8.
- Moore ZS, Seward JF, Lane JM. Smallpox. Lancet. 2006;367:425–35.
- Rotz LD, Dotson DA, Damon IK, Becher JA. Advisory Committee on Immunization Practices. Vaccinia (smallpox) vaccine: recommendations of the Advisory Committee on Immunization Practices (ACIP), 2001. MMWR Recomm Rep. 2001;50(RR-10):1–25 .
- Baxby D. Jenner’s smallpox vaccine. London: Heinemann Educational Books; 1981.
- Lewin PK. Mummified, frozen smallpox: is it a threat? JAMA. 1985;253:3095.
- Li Y, Olson VA, Laue T, Laker MT, Damon IK. Detection of monkeypox virus with real-time PCR assays. J Clin Virol. 2006;36:194–203.
- Dixon B. Doubts that reach out from the grave. New Sci. 1985;105:58–9.
- Fenner F. Can smallpox return? World Health Forum. 1987;8:297–304.
- Razzell P. Smallpox extinction: a note of caution. New Sci. 1976;71:35.
- A letter blamed for an epidemic of small-pox. New York Medical Journal. 1901;73:600.
- Boobbyer P. Small-pox in Nottingham. BMJ. 1901;1:1054.
- Rao AR. Infected inanimate objects (fomites) and their role in transmission of smallpox. WHO Document WHO/SE/7240. Geneva: World Health Organization; 1972.
- Frederiksen H, Motameni ST. The 1954–1955 epidemic of smallpox in Tabriz. Am J Trop Med Hyg. 1957;6:853–7 .
- Mitra AC, Sarkar JK, Mukherjee MK. Virus content of smallpox scabs. Bull World Health Organ. 1974;51:106–7 .
- Downie AW, Dumbell KR. Survival of variola virus in dried exudate and crusts from smallpox patients. Lancet. 1947;1:550–3.
- MacCallum FO, McDonald JR. Survival of variola virus in raw cotton. Bull World Health Organ. 1957;16:247–54 .
- Wolff HL, Croon JJ. The survival of smallpox virus (variola minor) in natural circumstances. Bull World Health Organ. 1968;38:492–3 .
- Huq F. Viability of variola virus in crusts at different temperatures and humidities. WHO document WHO/SE/7793. Geneva: World Health Organization; 1977.
- Dixon CW. Smallpox. London: J. & A. Churchill Ltd; 1962.
- Lewin PK. Mummy riddles unraveled. Bulletin of the Microscopical Society of Canada. 1984;12:3–8.
- Ruffer MA, Ferguson AR. Note on an eruption resembling that of variola in the skin of a mummy of the twentieth dynasty (1200–1100 B.C.). J Pathol. 1911;15:1–3.
- Marennikova SS, Shelukhina EM, Zhukova OA, Yanova NN, Loparev VN. Smallpox diagnosed 400 years later: results of skin lesions examination of 16th century Italian mummy. J Hyg Epidemiol Microbiol Immunol. 1990;34:227–31 .
- Fornaciari G, Marchetti A. Intact smallpox virus particles in an Italian mummy of sixteenth century. Lancet. 1986;2:625.
- Jackes MK. Osteological evidence for smallpox: a possible case from seventeenth century Ontario. Am J Phys Anthropol. 1983;60:75–81.
- Biagini P, Theves C, Balaresque P, Geraut A, Cannet C, Keyser C, Variola virus in a 300-year-old Siberian mummy. N Engl J Med. 2012;367:2057–9.
- Baxter PJ, Brazier AM, Young SE. Is smallpox a hazard in church crypts? Br J Ind Med. 1988;45:359–60 .
- Stone R. Public health: is live smallpox lurking in the Arctic? Science. 2002;295:2002.
- el-Mallakh RS. Night of the living dead: could the mummy strike again? JAMA. 1985;254:3038.
- Hopkins DR, Lane JM, Cummings EC, Millar JD. Two funeral-associated smallpox outbreaks in Sierra Leone. Am J Epidemiol. 1971;94:341–7 .
- Owsley DW, Bruwelheide KS, Cartmell LW, Burgess LE, Foote SJ, Chang SM, The man in the iron coffin: an interdisciplinary effort to name the past. Hist Archaeol. 2006;40:89–108.
- Razzell PE, Bradley L. The smallpox controversy. Local Popul Stud. 1974;12:42–4 .
- Arita I. Can we stop smallpox vaccination? World Health. 1980; (May):27–9 [cited 2013 Dec 2]. http://whqlibdoc.who.int/smallpox/WH_5_1980_p27.pdf
- Public health weekly reports for February 3, 1905. Public Health Rep. 1905;20:163–98 .
- Baxby D. The origins of vaccinia virus. J Infect Dis. 1977;136:453–5.
- Willerslev E, Cooper A. Ancient DNA. Proc Biol Sci. 2005;272:3–16.
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