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Volume 20, Number 4—April 2014
Dispatch

Pathology of US Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs

Kwonil Jung, Qiuhong Wang1Comments to Author , Kelly A. Scheuer, Zhongyan Lu, Yan Zhang, and Linda J. Saif1
Author affiliations: The Ohio State University, Wooster, Ohio, USA (K. Jung, Q. Wang, K.A. Scheuer, Z. Lu, L.J. Saif); Ohio Department of Agriculture, Reynoldsburg, Ohio, USA (Y. Zhang)

Main Article

Table 1

Design and results of a study of the pathology of US PEDV strain PC21A in gnotobiotic pigs, 2013*

Pig status, pig no.; age, d, at inoculation Inoculum passage no. Intranasal/oral inoculum, log10 GE Onset of clinical signs, PIH Viral RNA in serum sample at euthanasia, log10 GE/mL†
Fecal shedding, log10 GE/mL, by PIH
0 24 48 72 96
PEDV-inoculated
1; 10 1 7.3/7.7 <4.8† 10.7‡ – – – 25–26 7.6
2; 18 2 6.3/6.8 <4.8 <4.8 11.0 11.2‡ – 44–46 6.3
3; 24 3 8.3/8.8 <4.8 10.2 12.3‡ – – 44–46 5.7
4; 24§ 4 Indirect contact <4.8 10.9 9.6 10.6 ND 2–4§ 7.6
5; 35
 4
 0/9.0
 <4.8
 ND
 ND
 –
 –
 26–30
 4.8
Negative control
6; 26 . . <4.8 <4.8 <4.8 <4.8 <4.8 . <3.8†

*PEDV, porcine epidemic diarrhea virus; GE, genome equivalents; PIH, postinoculation hour; –, no result (pig euthanized); ND, not determined.
†Detected by real-time reverse transcription PCR with a detection limit of 4.8 log10 GE/mL for fecal samples and 3.8 log10 GE/mL for serum samples.
‡Euthanized.
§At 24 days of age, noninoculated pig 4 was exposed by indirect contact to pig 3 (at PIH 0) through small holes drilled into the stainless steel divider panel located between the 2 pigs in the shared pig tub isolator unit. Clinical signs and virus shedding were monitored after indirect contact. Diarrhea and vomiting developed in pig 4 approximately 2–4 h after clinical signs developed in pig 3 (i.e., in pig 4, signs developed 46–50 h after indirect contact with inoculated pig 3); pig 4 was euthanized ≈120 h after the onset of clinical signs.

Main Article

1These authors were co-principal investigators.

Page created: March 18, 2014
Page updated: March 18, 2014
Page reviewed: March 18, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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