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Volume 20, Number 4—April 2014
Dispatch

Pathology of US Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs

Kwonil Jung, Qiuhong Wang1Comments to Author , Kelly A. Scheuer, Zhongyan Lu, Yan Zhang, and Linda J. Saif1
Author affiliations: The Ohio State University, Wooster, Ohio, USA (K. Jung, Q. Wang, K.A. Scheuer, Z. Lu, L.J. Saif); Ohio Department of Agriculture, Reynoldsburg, Ohio, USA (Y. Zhang)

Main Article

Table 2

Histopathologic findings in a study of the pathology of US PEDV strain PC21A in gnotobiotic pigs, 2013*

Pig status, no. PIH at euthanasia (infection stage) VH:CD, mean (±SD) Antigen detection in frozen tissues†
Duodenum Jejunum Ileum Cecum/colon Lung
PEDV-inoculated
1 30 (acute) 3.4 (1.7) ++ +++ +++ +++ –
2 72 (mid) 1.8 (0.3) ++ +++ +++ +++ –
3 46 (acute) 1.2 (0.3) + ++ ++ ++ –
4‡ 120 (later)‡ 1.5 (0.2) + +/++ +/++ ++/+++ –
5
 67 (mid)
 2.2 (0.4)
 +
 +++
 +++
 +
 –
Negative control
6 . 6.3 (0.7) − − – – –

*PEDV, porcine epidemic diarrhea virus; PIH, postinoculation hour; VH:CD, ratio of villous height to crypt depth.
†Detected by immunofluorescence staining. +, 1%–29% of epithelial cells showed staining; ++, 30%–59% of epithelial cells showed staining; +++, 60%–100% of epithelial cells showed staining, as described (4); –, no cells showed staining.
‡At 24 days of age, noninoculated pig 4 was exposed by indirect contact to pig 3 (at PIH 0) through small holes drilled into the stainless steel divider panel located between the 2 pigs in the shared pig tub isolator unit. Clinical signs and virus shedding were monitored after indirect contact. Diarrhea and vomiting developed in pig 4 approximately 2–4 h after clinical signs developed in pig 3 (i.e., in pig 4, signs developed 46–50 h after indirect contact with inoculated pig 3); pig 4 was euthanized ≈120 h after the onset of clinical signs.

Main Article

References
  1. Cima  G. Fighting a deadly pig disease: industry, veterinarians trying to contain PED virus, new to the US. J Am Vet Med Assoc. 2013;243:469–70 .PubMedGoogle Scholar
  2. Stevenson  GW, Hoang  H, Schwartz  KJ, Burrough  EB, Sun  D, Madson  D, Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences. J Vet Diagn Invest. 2013;25:649–54 . DOIGoogle Scholar
  3. Debouck  P, Pensaert  M, Coussement  W. The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, Cv-777. Vet Microbiol. 1981;6:157–65. DOIGoogle Scholar
  4. Coussement  W, Ducatelle  R, Debouck  P, Hoorens  J. Pathology of experimental CV777 coronavirus enteritis in piglets. I. Histological and histochemical study. Vet Pathol. 1982;19:46–56 . DOIGoogle Scholar
  5. Sueyoshi  M, Tsuda  T, Yamazaki  K, Yoshida  K, Nakazawa  M, Sato  K, An immunohistochemical investigation of porcine epidemic diarrhoea. J Comp Pathol. 1995;113:59–67 . DOIGoogle Scholar
  6. Kim  O, Chae  C. In situ hybridization for the detection and localization of porcine epidemic diarrhea virus in the intestinal tissues from naturally infected piglets. Vet Pathol. 2000;37:62–7 . DOIGoogle Scholar
  7. Kim  L, Chang  KO, Sestak  K, Parwani  A, Saif  LJ. Development of a reverse transcription–nested polymerase chain reaction assay for differential diagnosis of transmissible gastroenteritis virus and porcine respiratory coronavirus from feces and nasal swabs of infected pigs. J Vet Diagn Invest. 2000;12:385–8 . DOIPubMedGoogle Scholar
  8. Amimo  JO, Vlasova  AN, Saif  LJ. Detection and genetic diversity of porcine group A rotaviruses in historic (2004) and recent (2011 and 2012) swine fecal samples in Ohio: predominance of the G9P[13] genotype in nursing piglets. J Clin Microbiol. 2013;51:1142–51 . DOIPubMedGoogle Scholar
  9. Jung  K, Wang  Q, Kim  Y, Scheuer  K, Zhang  Z, Shen  Q, The effects of simvastatin or interferon-alpha on infectivity of human norovirus using a gnotobiotic pig model for the study of antivirals. PLoS ONE. 2012;7:e41619 . DOIPubMedGoogle Scholar
  10. Kim  SH, Kim  IJ, Pyo  HM, Tark  DS, Song  JY, Hyun  BH. Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus. J Virol Methods. 2007;146:172–7 . DOIPubMedGoogle Scholar
  11. Jung  K, Kim  J, Ha  Y, Choi  C, Chae  C. The effects of transplacental porcine circovirus type 2 infection on porcine epidemic diarrhoea virus-induced enteritis in preweaning piglets. Vet J. 2006;171:445–50 . DOIPubMedGoogle Scholar
  12. Jung  K, Renukaradhya  GJ, Alekseev  KP, Fang  Y, Tang  Y, Saif  LJ. Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J Gen Virol. 2009;90:2713–23 . DOIPubMedGoogle Scholar
  13. Sisay  Z, Wang  Q, Oka  T, Saif  L. Prevalence and molecular characterization of porcine enteric caliciviruses and first detection of porcine kobuviruses in US swine. Arch Virol. 2013;158:1583–8 . DOIPubMedGoogle Scholar
  14. Wang  Q, Scheuer  K, Ahang  Z, Gebreyes  WA, Molla  BZ, Hoet  AE, Characterization and prevalence of a new porcine calicivirus in swine, United States. Emerg Infect Dis. 2011;17:1103–6 . DOIPubMedGoogle Scholar
  15. Chu  DK, Poon  LL, Guan  Y, Peiris  JS. Novel astroviruses in insectivorous bats. J Virol. 2008;82:9107–14 . DOIPubMedGoogle Scholar

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1These authors were co-principal investigators.

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Page updated: March 18, 2014
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