Volume 20, Number 5—May 2014
Research
Molecular Investigation of Tularemia Outbreaks, Spain, 1997–2008
Jaime Ariza-Miguel, Anders Johansson, María Isabel Fernández-Natal, Carmen Martínez-Nistal, Antonio Orduña, Elías F. Rodríguez-Ferri, Marta Hernández, and David Rodríguez-Lázaro
Table
Multilocus variable number tandem repeat analysis of 98 Francisella tularensis isolates from Spain and 11 reference isolates*
| MLVA genotype | No. isolates (origin) | No. MLVA markers that discriminated F. tularensis subsp. holarctica isolates |
||||
|---|---|---|---|---|---|---|
| Ft-M3 | Ft-M6 | Ft-M9 | Ft-M20 | Ft-M24† | ||
| A | 49 (Spain) | 5 | 4 | 2 | 3 | 2 (∆16 bp) |
| B | 32 (Spain) | 4 | 4 | 2 | 3 | 2 (∆16 bp) |
| C | 9 (Spain) | 6 | 4 | 2 | 3 | 2 (∆16 bp) |
| D | 3 (Spain) | 3 | 4 | 2 | 3 | 2 (∆16 bp) |
| E | 2 (Spain) | 4 | 7 | 2 | 3 | 2 (∆16 bp) |
| F | 1 (Spain) | 4 | 4 | 2 | 4 | 2 (∆16 bp) |
| G | 1 (Spain) | 4 | 4 | 3 | 3 | 2 (∆16 bp) |
| H | 1 (Spain) | 7 | 4 | 2 | 3 | 2 (∆16 bp) |
| I | 4 (former Czechoslovakia) | 4 | 6 | 2 | 3 | 2 |
| J | 2 (Czech Republic) | 5 | 7 | 2 | 3 | 2 |
| K | 2 (Czech Republic) | 6 | 7 | 2 | 3 | 2 |
| L | 1 (former Czechoslovakia) | 7 | 4 | 2 | 2 | 2 |
| M | 1 (Czech Republic) | 7 | 7 | 2 | 3 | 2 |
| N‡ | 1 (United States) | 28 | 4 | 4 | 3 | 1 |
*MLVA, multilocus variable number tandem repeat.
†All isolates from Spain had the unique 16-bp deletion at marker FT-M24 that is characteristic of genetic subclade B.Br:FTNF002–00 (Iberian clone).
‡Genotype N, F. tularensis subsp. tularensis strain Schu, showed additional unique alleles at the following MLVA markers: Ft-M5, Ft-M7, Ft-M8, Ft-M10, Ft-M13, Ft-M16, Ft-M19, Ft-M21, Ft-M22, and Ft-M23.
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