Volume 20, Number 5—May 2014
Molecular Investigation of Tularemia Outbreaks, Spain, 1997–2008
|MLVA genotype||No. isolates (origin)||No. MLVA markers that discriminated F. tularensis subsp. holarctica isolates
|A||49 (Spain)||5||4||2||3||2 (∆16 bp)|
|B||32 (Spain)||4||4||2||3||2 (∆16 bp)|
|C||9 (Spain)||6||4||2||3||2 (∆16 bp)|
|D||3 (Spain)||3||4||2||3||2 (∆16 bp)|
|E||2 (Spain)||4||7||2||3||2 (∆16 bp)|
|F||1 (Spain)||4||4||2||4||2 (∆16 bp)|
|G||1 (Spain)||4||4||3||3||2 (∆16 bp)|
|H||1 (Spain)||7||4||2||3||2 (∆16 bp)|
|I||4 (former Czechoslovakia)||4||6||2||3||2|
|J||2 (Czech Republic)||5||7||2||3||2|
|K||2 (Czech Republic)||6||7||2||3||2|
|L||1 (former Czechoslovakia)||7||4||2||2||2|
|M||1 (Czech Republic)||7||7||2||3||2|
|N‡||1 (United States)||28||4||4||3||1|
*MLVA, multilocus variable number tandem repeat.
†All isolates from Spain had the unique 16-bp deletion at marker FT-M24 that is characteristic of genetic subclade B.Br:FTNF002–00 (Iberian clone).
‡Genotype N, F. tularensis subsp. tularensis strain Schu, showed additional unique alleles at the following MLVA markers: Ft-M5, Ft-M7, Ft-M8, Ft-M10, Ft-M13, Ft-M16, Ft-M19, Ft-M21, Ft-M22, and Ft-M23.