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Volume 20, Number 6—June 2014
Research

Genetic Evidence of Importation of Drug-Resistant Plasmodium falciparum to Guatemala from the Democratic Republic of the Congo

Jaymin C. PatelComments to Author , Steve M. Taylor, Patricia C. Juliao, Christian M. Parobek, Mark Janko, Luis Demetrio Gonzalez, Lucia Ortiz, Norma Padilla, Antoinette K. Tshefu, Michael Emch, Venkatachalam Udhayakumar, Kim Lindblade, and Steven R. Meshnick
Author affiliations: University of North Carolina, Chapel Hill, North Carolina, USA (J.C. Patel, S.M. Taylor, C.M. Parobek, M. Janko, M. Emch, S.R. Meshnick); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (P.C. Juliao, V. Udhayakumar, K. Lindblade); Military Medical Center, Guatemala City, Guatemala (L.D. Gonzalez); Universidad de Valle de Guatemala, Guatemala City (L. Ortiz, N. Padilla); University of Kinshasa, Kinshasa, Democratic Republic of the Congo (A.K. Tshefu)

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Figure 4

Phylogenetic tree showing predicted clustering between Plasmodium falciparum populations from the Democratic Republic of Congo (DRC), soldiers returning to Guatemala from the DRC, and Guatemala. The predicted split between parasites identified in samples taken in Guatemala and parasites from DRC among soldiers was significant (95% CI >99.9%) (black bar); the predicted split between parasites from DRC and returning soldiers was not significant (95% CI 50%–70%) (white bar). Computed by using Fa

Figure 4. Phylogenetic tree showing predicted clustering between Plasmodium falciparum populations from the Democratic Republic of Congo (DRC), soldiers returning to Guatemala from the DRC, and GuatemalaThe predicted split between parasites identified in samples taken in Guatemala and parasites from DRC among soldiers was significant (95% CI >99.9%) (black bar); the predicted split between parasites from DRC and returning soldiers was not significant (95% CI 50%–70%) (white bar)Computed by using Fast UniFrac (30) with jacknifing and 1,000 permutations.

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References
  1. Feachem  RG, Phillips  AA, Hwang  J, Cotter  C, Wielgosz  B, Greenwood  BM, Shrinking the malaria map: progress and prospects. Lancet. 2010;376:1566–78. DOIPubMedGoogle Scholar
  2. Martens  P, Hall  L. Malaria on the move: human population movement and malaria transmission. Emerg Infect Dis. 2000;6:103–9. DOIPubMedGoogle Scholar
  3. Tatem  AJ, Smith  DL. International population movements and regional Plasmodium falciparum malaria elimination strategies. Proc Natl Acad Sci U S A. 2010;107:12222–7. DOIPubMedGoogle Scholar
  4. World Health Organization. World malaria report 2011. Geneva: The Organization; 2011. [cited 2014 Mar 28]. http://www.who.int/malaria/world_malaria_report_2011/en/
  5. National Center for Epidemiology, Ministry of Public Health and Social Assistance. 2010 Vital statistics, Guatemala [in Spanish] 2010 [cited 2014 Mar 27]. Ciudad de Guatemala: The Ministry. http://epidemiologia.mspas.gob.gt/vigepi/2010/MEMORIA%20REPUBLICA%20DE%20GUATEMALA%202010.pdf
  6. World Health Organization. Guidelines for the treatment of malaria, 2nd ed. Geneva: The Organization; 2010 [modified 2011 June 8, cited 2014 Mar 28]. http://whqlibdoc.who.int/publications/2010/9789241547925_eng.pdf?ua=1
  7. World Health Organization. Global report on antimalarial efficacy and drug resistance: 2000–2010. Geneva: The Organization; 2010 [cited 2014 Mar 28]. http://whqlibdoc.who.int/publications/2010/9789241500470_eng.pdf
  8. Mejia Torres  RE, Banegas  EI, Mendoza  M, Diaz  C, Bucheli  STM, Fontecha  GA, Efficacy of chloroquine for the treatment of uncomplicated Plasmodium falciparum malaria in Honduras. Am J Trop Med Hyg. 2013;88:850–4. DOIPubMedGoogle Scholar
  9. Juliao  PC, Sosa  S, Gonzalez  LD, Padilla  N, Ortiz  L, Goldman  I, Importation of chloroquine-resistant Plasmodium falciparum by Guatemalan peacekeepers returning from the Democratic Republic of the Congo. Malar J. 2013;12:344. DOIPubMedGoogle Scholar
  10. Rebaudet  S, Bogreau  H, Silai  R, Lepere  JF, Bertaux  L, Pradines  B, Genetic structure of Plasmodium falciparum and elimination of malaria, Comoros archipelago. Emerg Infect Dis. 2010;16:1686–94. DOIPubMedGoogle Scholar
  11. Schultz  L, Wapling  J, Mueller  I, Ntsuke  PO, Senn  N, Nale  J, Multilocus haplotypes reveal variable levels of diversity and population structure of Plasmodium falciparum in Papua New Guinea, a region of intense perennial transmission. Malar J. 2010;9:336. DOIPubMedGoogle Scholar
  12. Chwalisz  N, Kinosian  S. Guatemala sends Special Forces to D. R. Congo for UN peacekeeping mission. 2013 [cited 2013 July 15]. http://justf.org/blog/2013/08/29/guatemala-sends-special-forces-dr-congo-un-peacekeeping-mission
  13. Taylor  SM, Messina  JP, Hand  CC, Juliano  JJ, Muwonga  J, Tshefu  AK, Molecular malaria epidemiology: mapping and burden estimates for the Democratic Republic of the Congo, 2007. PLoS ONE. 2011;6:e16420. DOIPubMedGoogle Scholar
  14. Messina  JP, Taylor  SM, Meshnick  SR, Linke  AM, Tshefu  AK, Atua  B, Population, behavioural and environmental drivers of malaria prevalence in the Democratic Republic of Congo. Malar J. 2011;10:161. DOIPubMedGoogle Scholar
  15. Taylor  SM, van Eijk  AM, Hand  CC, Mwandagalirwa  K, Messina  JP, Tshefu  AK, Quantification of the burden and consequences of pregnancy-associated malaria in the Democratic Republic of the Congo. J Infect Dis. 2011;204:1762–71. DOIPubMedGoogle Scholar
  16. Taylor  SM, Antonia  AL, Parobek  CM, Juliano  JJ, Janko  M, Emch  M, Plasmodium falciparum sulfadoxine resistance is geographically and genetically clustered within the DR Congo. Scientific reports. 2013;3:1165.
  17. Herderschee  J, Kaiser  KA, Samba  DM. Resilience of an African giant: boosting growth and development in the Democratic Republic of Congo. Washington, (DC),The World Bank; 2012. 116 p.
  18. Nair  S, Williams  JT, Brockman  A, Paiphun  L, Mayxay  M, Newton  PN, A selective sweep driven by pyrimethamine treatment in southeast asian malaria parasites. Mol Biol Evol. 2003;20:1526–36. DOIPubMedGoogle Scholar
  19. Anderson  TJ, Haubold  B, Williams  JT, Estrada-Franco  JG, Richardson  L, Mollinedo  R, Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum. Mol Biol Evol. 2000;17:1467–82. DOIPubMedGoogle Scholar
  20. Anderson  TJ, Su  XZ, Bockarie  M, Lagog  M, Day  KP. Twelve microsatellite markers for characterization of Plasmodium falciparum from finger-prick blood samples. Parasitology. 1999;119:113–25. DOIPubMedGoogle Scholar
  21. Roper  C, Pearce  R, Bredenkamp  B, Gumede  J, Drakeley  C, Mosha  F, Antifolate antimalarial resistance in southeast Africa: a population-based analysis. Lancet. 2003;361:1174–81. DOIPubMedGoogle Scholar
  22. Balloux  F, Lugon-Moulin  N. The estimation of population differentiation with microsatellite markers. Mol Ecol. 2002;11:155–65. DOIPubMedGoogle Scholar
  23. Peakall  ROD, Smouse  PE. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes. 2006;6:288–95. DOIGoogle Scholar
  24. Hardy  OJ, Vekemans  X. SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes. 2002;2:618–20. DOIGoogle Scholar
  25. Excoffier  L, Laval  G, Schneider  S. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online. 2005;1:47–50 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2658868/.PubMedGoogle Scholar
  26. Saitou  N, Nei  M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406–25 http://mbe.oxfordjournals.org/content/4/4/406.long.PubMedGoogle Scholar
  27. Cavalli-Sforza  LL, Edwards  AW. Phylogenetic analysis. Models and estimation procedures. Am J Hum Genet. 1967;19:233–57 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1706274/.PubMedGoogle Scholar
  28. Populations v. 1.2.31 [Internet]. Hudson (MA): Bioinformatics Organization [updated 2010 Oct 20; cited 2013 June 9]. http://bioinformatics.org/~tryphon/populations/
  29. Paradis  E, Claude  J, Strimmer  K. APE: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004;20:289–90. DOIPubMedGoogle Scholar
  30. Hamady  M, Lozupone  C, Knight  R. Fast UniFrac: facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and PhyloChip data. ISME J. 2010;4:17–27. DOIPubMedGoogle Scholar
  31. Verhoef  L, Vennema  H, van Pelt  W, Lees  D, Boshuizen  H, Henshilwood  K, Use of norovirus genotype profiles to differentiate origins of foodborne outbreaks. Emerg Infect Dis. 2010;16:617–24 . DOIPubMedGoogle Scholar
  32. Barry  PM, Gardner  TJ, Funk  E, Oren  E, Field  K, Shaw  T, Multistate outbreak of MDR TB identified by genotype cluster investigation. Emerg Infect Dis. 2012;18:113–6. DOIPubMedGoogle Scholar
  33. Krause  G, Schoneberg  I, Altmann  D, Stark  K. Chemoprophylaxis and malaria death rates. Emerg Infect Dis. 2006;12:447–51. DOIPubMedGoogle Scholar
  34. The Global Health Group and the Malaria Atlas Project. Atlas of Malaria-eliminating countries. San Francisco: The Global Health Group, Global Health Sciences, University of California, San Francisco; 2011 [cited 2014 Mar 28]. http://www.malariaeliminationgroup.org/publications/atlas-of-malaria-eliminating-countries-2011
  35. The Global Fund to Fight AIDS. Tuberculosis and Malaria News Releases [Internet]. Ten countries rally to eliminate malaria in Central America and the Caribbean. San Jose, Costa Rica: The Global Fund to Fight AIDS, Tuberculosis and Malaria; 2013 Jun 28 [cited 2014 Mar 28]. http://www.theglobalfund.org/en/mediacenter/newsreleases/2013-06-28_Ten_Countries_Rally_to_Eliminate_Malaria_in_Central_America_and_the_Caribbean/
  36. Warren  M, Collins  WE, Richardson  BB, Skinner  JC. Morphologic variants of Anopheles albimanus and susceptibility to Plasmodium vivax and P. falciparum. Am J Trop Med Hyg. 1977;26:607–11 http://www.ajtmh.org/content/26/4/607.long.PubMedGoogle Scholar
  37. Baton  LA, Ranford-Cartwright  LC. Ookinete destruction within the mosquito midgut lumen explains Anopheles albimanus refractoriness to Plasmodium falciparum (3D7A) oocyst infection. Int J Parasitol. 2012;42:249–58. DOIPubMedGoogle Scholar
  38. Wilson  PE, Kazadi  W, Kamwendo  DD, Mwapasa  V, Purfield  A, Meshnick  SR. Prevalence of pfcrt mutations in Congolese and Malawian Plasmodium falciparum isolates as determined by a new Taqman assay. Acta Trop. 2005;93:97–106. DOIPubMedGoogle Scholar
  39. Gouandjika-Vasilache  I, Mazitchi  A, Gumede  N, Manirakiza  A, Manenegu  C, Koyazegbe  TD, Wild poliovirus importation, Central African Republic. Emerg Infect Dis. 2013;19:1012–3. DOIPubMedGoogle Scholar
  40. Odoom  JK, Forrest  L, Dunn  G, Osei-Kwasi  M, Obodai  E, Arthur-Quarm  J, Interruption of poliovirus transmission in Ghana: molecular epidemiology of wild-type 1 poliovirus isolated from 1995 to 2008. J Infect Dis. 2012;206:1111–20. DOIPubMedGoogle Scholar

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