To the Editor: Recent outbreaks of severe pneumonia or acute respiratory distress syndrome (ARDS) have attracted much public interest. Given current awareness levels, clinical personnel and health officials must rapidly and adequately respond to suspected outbreaks to prevent public disturbances. We report a case that highlights the potential of next-generation sequencing (NGS) to complement conventional diagnostics in such scenarios.

On March 29, 2013, a police officer (patient 1) was admitted to the emergency department of the University Medical Centre Hamburg–Eppendorf in Hamburg, Germany, because of ARDS. The patient was given mechanical ventilation; all diagnostic test results for pathogens commonly known to cause pneumonia were negative (www.virus-genomics.org/supplementaries/EID1406.pdf). Although treatment with antimicrobial drugs was immediately initiated, the patient died 6 days later of multiple organ failure.

On April 5, a second police officer (patient 2) from the same county was admitted to the same emergency department because of ARDS. He was transferred to the intensive care unit and given mechanical ventilation. Similar to the situation for patient 1, diagnostic test results were negative, prompting the news media to suspect an outbreak of a novel or mutated virus (1,2). Especially because of simultaneous outbreaks of avian influenza and infections with Middle East respiratory syndrome coronavirus in other parts of the world, these reports caused serious concern among the public and health officials.

After the death of patient 1 and hospitalization of patient 2, we subjected nucleic acids extracted from bronchoalveolar lavage (BAL) specimens from both patients to NGS by using a MiSeq sequencer (www.illumina.com/systems.ilmn). To enable rapid and unbiased detection of bacterial and viral agents, we did not enrich specific sequences. The entire workflow (www.virus-genomics.org/supplementaries/EID1406.pdf) was completed within 50 hours.

First-line analysis clearly identified Chlamydophila psittaci in the RNA sample from patient 2, but no sequences of obvious pathogenic origin were detected in samples from patient 1 (Figure). C. psittaci, an intracellular bacterium, can be transmitted by inhaling aerosolized secretions or feces from infected birds (3). Person-to-person transmission of this bacterium is rare (4,5). Ornithosis, a disease characterized by severe pneumonia and influenza-like symptoms, might develop in persons infected with this bacterium. Because of the rarity of the disease, standard diagnostic panels usually do not include C. psittaci. After 11 days of antimicrobial drug treatment, the condition of patient 2 improved, and the patient was transferred to a general hospital ward.

On April 29, a third police officer (patient 3) who had been sharing office space with patient 2, came to the hospital with symptoms of pneumonia. After antimicrobial drug treatment, his condition rapidly improved and the patient was discharged on day 7.

At admission of patient 3, samples from all 3 patients were analyzed at greater read depth by using a HiSeq system. RNA sequencing identified commensal bacteria in all samples, but C. psittaci was present only in samples from patient 2 (Figure). Likewise, subsequent high-throughput 16S rRNA sequencing (6), PCR, and serologic analysis unequivocally identified a C. psittaci infection in patient 2, but not in patients 1 or 3 (www.virus-genomics.org/supplementaries/EID1406.pdf).

We did not detect viral pathogens in any samples. At the DNA level, most nonhost reads originated from nonpathogenic single-stranded DNA anelloviruses (7). No RNA viruses were found, although influenza A(H3N2) virus was readily identified in a MiSeq analysis of a control BAL sample from a patient with a diagnosis of influenza A (PCR cycle threshold 32) (Figure). Furthermore, pairwise BLAST analysis (http://blast.ncbi.nlm.nih.gov/Blast.cgi) did not reveal the presence of unknown sequence contigs that were shared among the patients, as would be expected in case of infection with a novel viral agent. Together with the confirmed C. psittaci infection in patient 2, the absence of a common pathogen signature strongly suggests that the cases were unrelated.

We used a comprehensive metagenomic approach to resolve cases suspected of representing an ongoing outbreak. The method used enabled diagnosis of a C. psittaci infection within a reasonable timeframe to allow for timely clinical intervention. These findings strongly suggest that NGS methods can complement conventional diagnostics (8–10) and also highlight their potential to aid clinical personnel and health agencies in making appropriate decisions during suspected outbreaks. Clearly, however, NGS-based methods will have to be further standardized and validated before their full potential in diagnostic settings can be realized.

The absence of pathogenic sequences in patients 1 and 3 might suggest that their clinical symptoms had noninfectious causes. However, although samples were collected during the acute phase of clinical symptoms, and despite our ability to detect an influenza A infection in controls, we cannot fully exclude the possibility that a potentially causative pathogen present at low levels might have evaded detection. Thus, systematic and correlative studies evaluating the sensitivity of NGS-based detection methods in different diagnostic entities are urgently needed.

Top

Nicole Fischer¹, Holger Rohde¹, Daniela Indenbirken, Thomas Günther, Kerstin Reumann, Marc Lütgehetmann, Thomas Meyer, Stefan Kluge, Martin Aepfelbacher, Malik Alawi, and Adam Grundhoff 

Author affiliations: University Medical Centre Hamburg–Eppendorf, Hamburg, Germany (N. Fischer, H. Rohde, M. Lütgehetmann, T. Meyer, S. Kluge, M. Aepfelbacher, M. Alawi); German Center for Infection Research, Hamburg (N. Fischer, A. Grundhoff); Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg (D. Indenbirken, T. Günther, K. Reumann, M. Alawi, A. Grundhoff)

Related Links

• More Letter Articles