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Volume 22, Number 11—November 2016
Letter

Spotted Fever Group Rickettsia in the Pampa Biome, Brazil, 2015–2016

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To the Editor: Several cases of tickborne rickettsiosis have been reported in South America in recent years (1,2). In Brazil, 2 spotted fever group (SFG) Rickettsia species, R. rickettsii and Rickettsia sp. strain Atlantic Rainforest, have been identified as causes of human disease. Rio Grande do Sul is the southernmost state of Brazil and the only part of the country located in the Pampa biome. Despite confirmed cases of human spotted fever in that state since 2005, little information is available regarding Rickettsia species. We report an eco-epidemiologic investigation of R. parkeri in Amblyomma tigrinum ticks on dogs from a household (and neighborhood) where a case of human spotted fever was diagnosed.

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Thumbnail of Setting for investigation of human infection with spotted fever group Rickettsia in the Pampa biome, Brazil, 2015–2016. A) Rio Grande do Sul state, Brazil, and neighboring countries. Light gray shading indicates the Pampa biome; dark gray shading indicates bodies of water; dotted line indicates Rosário do Sul municipality; black triangle indicates patient’s household. B) Typical view of Pampa vegetation (and area used for hunting by patient). C) Patient’s home. D) The patient and 1

Figure. Setting for investigation of human infection with spotted fever group Rickettsia in the Pampa biome, Brazil, 2015–2016. A) Rio Grande do Sul state, Brazil, and neighboring countries. Light gray shading indicates...

In 2011, a 44-year-old woman from the municipality of Rosário do Sul in Rio Grande do Sul (Figure) sought medical attention at the municipal health center. On examination, she had a cutaneous eschar, fever, malaise, lymphadenopathy, myalgia, headache, and rash; she reported receiving a tick bite a few days before. The diagnosis of spotted fever was confirmed at the Brazil National Reference Laboratory (Instituto Adolfo Lutz) in São Paulo after paired serologic testing (21-day interval) against R. rickettsii (first antibody titration 1:64; second 1:256) because the official diagnosis of human spotted fever in Brazil is based on serologic testing using only the R. rickettsii antigen. After doxycycline treatment (2×/d for 7 d), the patient had a complete recovery.

During September 2015–March 2016, we performed tick collections at the patient’s house, in the surrounding neighborhood (i.e., 7 other homes located within a radius of 1 km), and in the venues used by the patient for hunting. The patient and 11 relatives lived in a small house under extremely poor economic and sanitary conditions. They survived exclusively by government social programs and illegal hunting. The patient usually hunted several wild animals, including capybaras (Hydrochoerus hydrochaeris), armadillos (Dasypus spp.), the pampas fox (Lycalopex gymnocercus), and the crab-eating fox (Cerdocyon thous). We collected 251 Amblyomma dubitatum ticks from capybaras carcasses (74 adults and 173 nymphs) and from vegetation by dragging/flagging (2 adults and 2 nymphs); 60 Amblyomma sp. larvae were obtained by dragging/flagging. We obtained 47 adult A. tigrinum ticks and 2 adult Rhipicephalus sanguineus ticks from 14 owned free-roaming dogs with permanent access to wild habitats. We obtained ticks from the patient’s 8 dogs and from 6 other dogs from among 3 other households.

We taxonomically identified the ticks by morphology (3), processed whole ticks individually to obtain genomic DNA (4), and used PCR amplication of the rickettsial citrate synthase gene (gltA) as a screening procedure (5). We further tested tick samples that were positive for Rickettsia spp. by gltA PCR by using a second PCR, which amplified a fragment of the ompA gene from SFG Rickettsia spp. (6). We then tested positive samples a third time by using PCR amplification of a htrA gene fragment (5,7). PCR products of the ompA and htrA genes were purified and sequenced and then compared with sequences available in GenBank. All samples of A. dubitatum ticks were negative. Of the ticks collected from dogs, 13 A. tigrinum (28%) and 1 R. sanguineus (1/2) were positive in all PCR analyses (gltA, ompA, and htrA); 11 of these ticks were from the patient’s dogs. In all properties where ticks were collected, at least 1 was PCR positive. Thus, we detected R. parkeri in half (4/8) of investigated households.

All the sequences generated for the ompA and htrA genes showed 100% identity to sequences from the Rickettsia parkeri strain Portsmouth (GenBank accession no. CP003341.1). We deposited into GenBank the sequences of the ompA gene (KX196265) and htrA gene (KX196266) from samples analyzed in this study. The ompA sequence we obtained for R. parkeri showed 98% identity with Rickettsia sp. strain Atlantic Rainforest (GenBank accession no. GQ855237.1).

Although Rickettsia sp. strain Atlantic Rainforest had previously been considered the only SFG Rickettsia in southern Brazil, we demonstrate here the presence of R. parkeri in Rio Grande do Sul in the Pampa biome. We detected R. parkeri infection in A. tigrinum ticks collected at the probable site of infection (the patient’s home) of a confirmed case of human spotted fever. Considering the A. tigrinum tick abundance in southern Brazil and its remarkable ability to parasitize domestic and wild animals (8), in addition to the high R. parkeri infection rate observed (28%), further epidemiologic studies are needed to address the role of A. tigrinum ticks as vector of spotted fever in the Pampa biome. Finally, our results show that, in addition to R. rickettsii and Rickettsia sp. strain Atlantic Rainforest, R. parkeri occurs and might be associated with cases of spotted fever in Brazil. Additional surveys are needed to assess the infection prevalence of R. parkeri in A. tigrinum ticks in other areas of Pampa and in other regions of Brazil.

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Acknowledgment

This work was supported by the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and INCT Entomologia Molecular.

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Bárbara Weck, Bruno Dall’Agnol, Ugo Souza, Anelise Webster, Barbara Stenzel, Guilherme Klafke, João Ricardo Martins, and José ReckComments to Author 

Author affiliations: Instituto de Pesquisas Veterinárias Desidério Finamor, Eldorado do Sul, Brazil (B. Weck, B. Dall’Agnol, U. Souza, A. Webster, G. Klafke, J.R. Martins, J. Reck); Centro Estadual de Vigilância em Saúde, Porto Alegre, Brazil (B. Stenzel)

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References

  1. Galvão  MA, Mafra  CL, Moron  C, Anaya  E, Walker  DH. Rickettsiosis of the genus Rickettsia in South America. Ann N Y Acad Sci. 2003;990:5761.DOIPubMed
  2. Parola  P, Labruna  MB, Raoult  D. Tick-borne rickettsioses in America: unanswered questions and emerging diseases. Curr Infect Dis Rep. 2009;11:4050.DOIPubMed
  3. Barros-Battesti  DM, Arzua  M, Bechara  GH. Carrapatos de Importância Médico-Veterinária da Região Neotropical: um guia ilustrado para identificação de espécies. São Paulo (Brazil): Vox/ICTTD-3/Butantan; 2006.
  4. Aljanabi  SM, Martinez  I. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res. 1997;25:46923.DOIPubMed
  5. Labruna  MB, Whitworth  T, Horta  MC, Bouyer  DH, McBride  JW, Pinter  A, Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of São Paulo, Brazil, where Brazilian spotted fever is endemic. J Clin Microbiol. 2004;42:908.DOIPubMed
  6. Regnery  RL, Spruill  CL, Plikaytis  BD. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol. 1991;173:157689.PubMed
  7. Azad  AF, Webb  L, Carl  M, Dasch  GA. Detection of rickettsiae in arthropod vectors by DNA amplification using the polymerase chain reaction. Ann N Y Acad Sci. 1990;590:55763.DOIPubMed
  8. Evans  DE, Martins  JR, Guglielmone  AA. A review of the ticks (Acari, ixodida) of Brazil, their hosts and geographic distribution - 1. The state of Rio Grande do Sul, southern Brazil. Mem Inst Oswaldo Cruz. 2000;95:45370.DOIPubMed

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Cite This Article

DOI: 10.3201/eid2211.160859

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Table of Contents – Volume 22, Number 11—November 2016

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José Reck, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde, 6000, Eldorado do Sul, 92990-000, RS, Brazil

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Page created: October 19, 2016
Page updated: October 19, 2016
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