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Volume 22, Number 3—March 2016
Letter

Mycobacterium orygis–Associated Tuberculosis in Free-Ranging Rhinoceros, Nepal, 2015

Jeewan Thapa1, Sarad Paudel1, Amir Sadaula, Yogendra Shah, Bhagwan Maharjan, Gretchen E. Kaufman, Deborah McCauley, Kamal P. Gairhe, Toshio Tsubota, Yasuhiko Suzuki, and Chie NakajimaComments to Author 
Author affiliations: Hokkaido University, Sapporo, Japan (J. Thapa, S. Paudel, Y. Shah, T. Tsubota, Y. Suzuki, C. Nakajima); National Trust for Nature Conservation, Biodiversity Conservation Center, Chitwan, Nepal (A. Sadaula); German Nepal Tuberculosis Project, Kathmandu, Nepal (B. Maharjan); Veterinary Initiative for Endangered Wildlife, Bozeman, Montana, USA (G.E. Kaufman, D. McCauley); Chitwan National Park Department of National Parks and Wildlife Conservation, Chitwan (K.P. Gairhe)

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Figure

Phylogeny of Mycobacterium orygis isolates as determine on the basis of mycobacterial interspersed repetitive units–variable-number tandem-repeat (MIRU-VNTR) results of 22 loci. The unweighted pair group method with arithmetic mean dendrogram was drawn by using MIRU-VNTRplus software (http://www.miru-vntrplus.org). The order of MIRU-VNTR is as follows, left to right: 154, 424, 577, 580, 802, 960, 1644, 1955, 2059, 2163b, 2165, 2401, 2461, 2531, 2687, 2996, 3007, 3192, 3690, 4052, 4156 and 4348.

Figure. Phylogeny of Mycobacterium orygis isolates as determine on the basis of mycobacterial interspersed repetitive units–variable-number tandem-repeat (MIRU-VNTR) results of 22 loci. The unweighted pair group method with arithmetic mean dendrogram was drawn by using MIRU-VNTRplus software (http://www.miru-vntrplus.org). The order of MIRU-VNTR is as follows, left to right: 154, 424, 577, 580, 802, 960, 1644, 1955, 2059, 2163b, 2165, 2401, 2461, 2531, 2687, 2996, 3007, 3192, 3690, 4052, 4156 and 4348. *Isolates from (1), †isolate from (8), ‡isolates from (2), §isolate from this study. Bold MIRU-VNTR copy number of locus 424 in rhinoceros isolate indicates a single locus difference in MIRU-VNTR type from the largest cluster. X, unamplifiable. Scale bar indicates genetic distance

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1These first authors contributed equally to this article.

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