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Volume 22, Number 4—April 2016

Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002–20121

Anne Kvistholm JensenComments to Author , Jonas T. Björkman, Steen Ethelberg, Kristoffer Kiil, Michael Kemp, and Eva Møller Nielsen
Author affiliations: Statens Serum Institut, Copenhagen, Denmark (A.K. Jensen, J.T. Björkman, S. Ethelberg, K. Kiil, E.M. Nielsen); University of Southern Denmark, Odense, Denmark (A.K. Jensen, M. Kemp); Odense University Hospital, Odense (M. Kemp)

Main Article

Table 3

Clusters of identical pulsotypes of Listeria monocytogenes isolates, Denmark, 2002–2012*

Year(s) No. cases Time range for the cluster, d Genetic lineage (PCR serogroup) ST/CC Pulsotype
Clusters with pulsotypes other than the 3 highly frequent types
2003† 3 40 I (ND) 224/224 181–218
2004 3 90 I (IVb) 6/6 27–20
2005‡ 3 97 I (ND) 5/5 31–30
2005–2006 3 42 I (IVb) 1/1 25–1
2006 3 66 II (IIa) 155/155 44–61
2006† 4 103 II (ND) 391/89 21–34
2009 4 41 II (IIa) 155/155 43–63
2009†§ 8 5 II (IIa) 9/9 71–43
2009 4 130 I (IIb) 59/59 35–26
2009 3 97 I (IVb) 1/1 67–92
2010 3 60 II (IIc) 9/9 41–43
2010 3 28 I (IVb) 1/1 22–2
II (IIa)
Clusters with the 3 highly frequent pulsotypes
2004 3 84 II (IIa) 8/8 38–48
2005 3 81 II (IIa) 8/8 38–48
2006–2007 11 191 II (IIa) 8/8 38–48
2007 6 164 II (IIa) 8/8 38–48
2008 3 81 II (IIa) 8/8 38–48
2005 5 86 II (IIa) 8/8 40–42
2006–2007 12 230 II (IIa) 8/8 40–42
2007–2008 23 421 II (IIa) 8/8 40–42
2008–2009 6 83 II (IIa) 8/8 40–42
2009–2010 15 310 II (IIa) 8/8 40–42
2010 3 44 II (IIa) 8/8 40–42
2011 3 55 II (IIa) 8/8 40–42
2012 3 75 II (IIa) 8/8 40–42
2007 3 46 I (IVb) 2/2 3–23
2009–2010¶ 8 148 I (IVb) 2/2 3–23
2010# 19 253 I (IVb) 2/2 3–23

*A cluster was defined as >3 cases with identical pulsotypes within 14 weeks. ST, sequence type; CC, clonal complex; ND not determined.
†This type only seen this 1 time during the study period.
‡This type only seen in 2005.
§Confirmed outbreak with meals-on-wheels food delivery as source of infection, described previously (15).
¶Cluster investigated but no common source identified.
#May be part of the former cluster (marked with ¶) with this pulsotype 3–23.The cluster included 5 pregnancy-associated cases. Cluster investigation could not determine a common source of infection, but smoked salmon was suspected based upon interview data.

Main Article

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Main Article

1Preliminary results from this study were presented at the 18th International Symposium on Problems of Listeriosis (ISOPOL XVIII), September 19–22, 2013, Goa, India.

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