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Volume 22, Number 4—April 2016
Research

Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002–20121

Anne Kvistholm JensenComments to Author , Jonas T. Björkman, Steen Ethelberg, Kristoffer Kiil, Michael Kemp, and Eva Møller Nielsen
Author affiliations: Statens Serum Institut, Copenhagen, Denmark (A.K. Jensen, J.T. Björkman, S. Ethelberg, K. Kiil, E.M. Nielsen); University of Southern Denmark, Odense, Denmark (A.K. Jensen, M. Kemp); Odense University Hospital, Odense (M. Kemp)

Main Article

Table 3

Clusters of identical pulsotypes of Listeria monocytogenes isolates, Denmark, 2002–2012*

Year(s) No. cases Time range for the cluster, d Genetic lineage (PCR serogroup) ST/CC Pulsotype
Clusters with pulsotypes other than the 3 highly frequent types
2003† 3 40 I (ND) 224/224 181–218
2004 3 90 I (IVb) 6/6 27–20
2005‡ 3 97 I (ND) 5/5 31–30
2005–2006 3 42 I (IVb) 1/1 25–1
2006 3 66 II (IIa) 155/155 44–61
2006† 4 103 II (ND) 391/89 21–34
2009 4 41 II (IIa) 155/155 43–63
2009†§ 8 5 II (IIa) 9/9 71–43
2009 4 130 I (IIb) 59/59 35–26
2009 3 97 I (IVb) 1/1 67–92
2010 3 60 II (IIc) 9/9 41–43
2010 3 28 I (IVb) 1/1 22–2
2011
4
108
II (IIa)
18/18
101–126
Clusters with the 3 highly frequent pulsotypes
2004 3 84 II (IIa) 8/8 38–48
2005 3 81 II (IIa) 8/8 38–48
2006–2007 11 191 II (IIa) 8/8 38–48
2007 6 164 II (IIa) 8/8 38–48
2008 3 81 II (IIa) 8/8 38–48
2005 5 86 II (IIa) 8/8 40–42
2006–2007 12 230 II (IIa) 8/8 40–42
2007–2008 23 421 II (IIa) 8/8 40–42
2008–2009 6 83 II (IIa) 8/8 40–42
2009–2010 15 310 II (IIa) 8/8 40–42
2010 3 44 II (IIa) 8/8 40–42
2011 3 55 II (IIa) 8/8 40–42
2012 3 75 II (IIa) 8/8 40–42
2007 3 46 I (IVb) 2/2 3–23
2009–2010¶ 8 148 I (IVb) 2/2 3–23
2010# 19 253 I (IVb) 2/2 3–23

*A cluster was defined as >3 cases with identical pulsotypes within 14 weeks. ST, sequence type; CC, clonal complex; ND not determined.
†This type only seen this 1 time during the study period.
‡This type only seen in 2005.
§Confirmed outbreak with meals-on-wheels food delivery as source of infection, described previously (15).
¶Cluster investigated but no common source identified.
#May be part of the former cluster (marked with ¶) with this pulsotype 3–23.The cluster included 5 pregnancy-associated cases. Cluster investigation could not determine a common source of infection, but smoked salmon was suspected based upon interview data.

Main Article

References
  1. Smith  B, Kemp  M, Ethelberg  S, Schiellerup  P, Bruun  BG, Gerner-Smidt  P, Listeria monocytogenes: maternal-foetal infections in Denmark 1994–2005. Scand J Infect Dis. 2009;41:215. DOIPubMed
  2. Cartwright  EJ, Jackson  KA, Johnson  SD, Graves  LM, Silk  BJ, Mahon  BE. Listeriosis outbreaks and associated food vehicles, United States, 1998–2008. Emerg Infect Dis. 2013;19:19. DOIPubMed
  3. European Food Safety Authority, European Center for Disease Prevention and Control. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013. EFSA J. 2015;13:3991.
  4. European Food Safety Authority, European Center for Disease Prevention and Control. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010. EFSA J. 2012;10:1442.
  5. Félix  B, Niskanen  T, Vingadassalon  N, Dao  TT, Asséré  A, Lombard  B, Pulsed-field gel electrophoresis proficiency testing trials: toward European harmonization of the typing of food and clinical strains of Listeria monocytogenes. Foodborne Pathog Dis. 2013;10:87381. DOIPubMed
  6. Graves  LM, Swaminathan  B. PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. Int J Food Microbiol. 2001;65:5562. DOIPubMed
  7. Orsi  RH, den Bakker  HC, Wiedmann  M. Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int J Med Microbiol. 2011;301:7996. DOIPubMed
  8. Statens Serum Institut. Listeriosis 2006–2013. EPI-NEWS. 2014 [cited 2015 May 26]. http://www.ssi.dk/English/News/EPI-NEWS/2014/No%2018%20-%202014.aspx
  9. Kvistholm Jensen  A, Ethelberg  S, Smith  B, Moller Nielsen  E, Larsson  J, Molbak  K, Substantial increase in listeriosis, Denmark 2009. Euro Surveill. 2010;15:14 .PubMed
  10. European Commission. Commission decision of 28 April 2008 amending Decision 2002/253/EC laying down case definitions for reporting communicable diseases to the Community network under Decision No 2119/98/EC of the European Parliament and of the Council [cited 2015 May 26]. http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32008D0426&qid=1433247163665&from=EN
  11. Doumith  M, Jacquet  C, Gerner-Smidt  P, Graves  LM, Loncarevic  S, Mathisen  T, Multicenter validation of a multiplex PCR assay for differentiating the major Listeria monocytogenes serovars 1/2a, 1/2b, 1/2c, and 4b: toward an international standard. J Food Prot. 2005;68:264850 .PubMed
  12. Ragon  M, Wirth  T, Hollandt  F, Lavenir  R, Lecuit  M, Le Monnier  A, A new perspective on Listeria monocytogenes evolution. PLoS Pathog. 2008;4:e1000146. DOIPubMed
  13. Cantinelli  T, Chenal-Francisque  V, Diancourt  L, Frezal  L, Leclercq  A, Wirth  T, “Epidemic clones” of Listeria monocytogenes are widespread and ancient clonal groups. J Clin Microbiol. 2013;51:37709. DOIPubMed
  14. Goulet  V, Jacquet  C, Martin  P, Vaillant  V, Laurent  E, de Valk  H. Surveillance of human listeriosis in France, 2001–2003. Euro Surveill. 2006;11:7981 .PubMed
  15. Smith  B, Larsson  JT, Lisby  M, Müller  L, Madsen  SB, Engberg  J, Outbreak of listeriosis caused by infected beef meat from a meals-on-wheels delivery in Denmark 2009. Clin Microbiol Infect. 2011;17:502. DOIPubMed
  16. Haase  JK, Didelot  X, Lecuit  M, Korkeala  H, Achtman  M, Leclercq  A, The ubiquitous nature of Listeria monocytogenes clones: A large-scale multilocus sequence typing study. Environ Microbiol. 2014;16:40516. DOIPubMed
  17. Althaus  D, Lehner  A, Brisse  S, Maury  M, Tasara  T, Stephan  R. Characterization of Listeria monocytogenes strains isolated during 2011–2013 from human infections in Switzerland. Foodborne Pathog Dis. 2014;11:7538. DOIPubMed
  18. Knabel  SJ, Reimer  A, Verghese  B, Lok  M, Ziegler  J, Farber  J, Sequence typing confirms that a predominant Listeria monocytogenes clone caused human listeriosis cases and outbreaks in Canada from 1988 to 2010. J Clin Microbiol. 2012;50:174851 . DOIPubMed
  19. Gilmour  MW, Graham  M, Van Domselaar  G, Tyler  S, Kent  H, Trout-Yakel  KM, High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak. BMC Genomics. 2010;11:120. DOIPubMed
  20. Verghese  B, Lok  M, Wen  J, Alessandria  V, Chen  Y, Kathariou  S, comK prophage junction fragments as markers for Listeria monocytogenes genotypes unique to individual meat and poultry processing plants and a model for rapid niche-specific adaptation, biofilm formation, and persistence. Appl Environ Microbiol. 2011;77:327992. DOIPubMed
  21. Nakari  UM, Rantala  L, Pihlajasaari  A, Toikkanen  S, Johansson  T, Hellsten  C, Investigation of increased listeriosis revealed two fishery production plants with persistent Listeria contamination in Finland in 2010. Epidemiol Infect. 2014;142:22619. DOIPubMed
  22. Miettinen  H, Wirtanen  G. Ecology of Listeria spp. in a fish farm and molecular typing of Listeria monocytogenes from fish farming and processing companies. Int J Food Microbiol. 2006;112:13846. DOIPubMed
  23. Katzav  M, Hyvönen  P, Muje  P, Rantala  L, Von Wright  A. Pulsed-field gel electrophoresis typing of Listeria monocytogenes isolated in two Finnish fish farms. J Food Prot. 2006;69:14437 .PubMed
  24. European Food Safety Authority. Analysis of the baseline survey on the prevalence of Listeria monocytogenes in certain ready-to-eat foods in the EU, 2010–2011 Part A: Listeria monocytogenes prevalence estimates. EFSA J. 2013;11:3241.
  25. Lambertz  ST, Ivarsson  S, Lopez-Valladares  G, Sidstedt  M, Lindqvist  R. Subtyping of Listeria monocytogenes isolates recovered from retail ready-to-eat foods, processing plants and listeriosis patients in Sweden 2010. Int J Food Microbiol. 2013;166:18692. DOIPubMed
  26. Lunestad  BT, Truong  TTT, Lindstedt  BA. A multiple-locus variable-number tandem repeat analysis (MLVA) of Listeria monocytogenes isolated from Norwegian salmon-processing factories and from listeriosis patients. Epidemiol Infect. 2013;141:210110. DOIPubMed
  27. Barrett  TJ, Gerner-Smidt  P, Swaminathan  B. Interpretation of pulsed-field gel electrophoresis patterns in foodborne disease investigations and surveillance. Foodborne Pathog Dis. 2006;3:2031. DOIPubMed
  28. Sauders  BD, Schukken  Y, Kornstein  L, Reddy  V, Bannerman  T, Salehi  E, Molecular epidemiology and cluster analysis of human listeriosis cases in three U.S. states. J Food Prot. 2006;69:16809 .PubMed
  29. Orsi  RH, Borowsky  ML, Lauer  P, Young  SK, Nusbaum  C, Galagan  JE, Short-term genome evolution of Listeria monocytogenes in a non-controlled environment. BMC Genomics. 2008;9:539. DOIPubMed
  30. Kathariou  S, Graves  L, Buchrieser  C, Glaser  P, Siletzky  RM, Swaminathan  B. Involvement of closely related strains of a new clonal group of Listeria monocytogenes in the 1998–99 and 2002 multistate outbreaks of foodborne listeriosis in the United States. Foodborne Pathog Dis. 2006;3:292302. DOIPubMed
  31. Rychli  K, Müller  A, Zaiser  A, Schoder  D, Allerberger  F, Wagner  M, Genome sequencing of Listeria monocytogenes “Quargel” listeriosis outbreak strains reveals two different strains with distinct in vitro virulence potential. PLoS ONE. 2014;9:e89964. DOIPubMed
  32. McCollum  JT, Cronquist  AB, Silk  BJ, Jackson  KA, O’Connor  KA, Cosgrove  S, Multistate outbreak of listeriosis associated with cantaloupe. N Engl J Med. 2013;369:94453. DOIPubMed
  33. Centers for Disease Control and Prevention. Vital signs: listeria illnesses, deaths, and outbreaks—United States, 2009–2011. MMWR Morb Mortal Wkly Rep. 2013;62:44852 .PubMed
  34. Gillespie  IA, McLauchlin  J, Little  CL, Penman  C, Mook  P, Grant  K, Disease presentation in relation to infection foci for non-pregnancy-associated human listeriosis in England and Wales, 2001 to 2007. J Clin Microbiol. 2009;47:33017. DOIPubMed
  35. Goulet  V, Hedberg  C, Le Monnier  A, de Valk  H. Increasing incidence of listeriosis in France and other European countries. Emerg Infect Dis. 2008;14:73440. DOIPubMed
  36. Mammina  C, Parisi  A, Guaita  A, Aleo  A, Bonura  C, Nastasi  A, Enhanced surveillance of invasive listeriosis in the Lombardy region, Italy, in the years 2006–2010 reveals major clones and an increase in serotype 1/2a. BMC Infect Dis. 2013;13:152. DOIPubMed
  37. Gerner-Smidt  P, Ethelberg  S, Schiellerup  P, Christensen  JJ, Engberg  J, Fussing  V, Invasive listeriosis in Denmark 1994–2003: a review of 299 cases with special emphasis on risk factors for mortality. Clin Microbiol Infect. 2005;11:61824. DOIPubMed
  38. Chenal-Francisque  V, Lopez  J, Cantinelli  T, Caro  V, Tran  C, Leclercq  A, Worldwide distribution of major clones of Listeria monocytogenes. Emerg Infect Dis. 2011;17:11102. DOIPubMed

Main Article

1Preliminary results from this study were presented at the 18th International Symposium on Problems of Listeriosis (ISOPOL XVIII), September 19–22, 2013, Goa, India.

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