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Volume 22, Number 4—April 2016
Letter

Follow-up of Ebola Patient, 2014–2015

Venkatesh Srinivas, Pragya D. Yadav, Veena Mittal, Gouri Chaubal, Mala Chhabra, Sanjay Mattoo, Arvind Rai, and Devendra T. MouryaComments to Author 
Author affiliations: National Centre for Disease Control, New Delhi, India (S. Venkatesh, V. Mittal, M. Chhabra, A. Rai); National Institute of Virology, Pune, India (P. Yadav, G. Chaubal, D.T. Mourya); Airport Authority of India, New Delhi (S. Matto)

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Table

Results of attempted real-time RT-PCR and virus isolation from semen of Ebola virus disease survivor, 2014–2015*

1
2
3
4
NCDC‡
NIV§
CPE
Ct
CPE
Ct
CPE
Ct
CPE
Ct
1
45
17
22.5
Vero CCL81 None 28.5 None None None None None None
Vero E6
None
31.5

None
None

None
None

ND
None
2
64
21
24
Vero CCL81 None None None None None None ND ND
Vero E6
None
32.5

None
None

None
None

ND
ND

77
22
ND
NA
NA
NA

NA
NA

NA
NA

NA
NA

98
26
ND
NA
NA
NA

NA
NA

NA
NA

NA
NA

111
27
ND
NA
NA
NA

NA
NA

NA
NA

NA
NA
6
125
30
34
Vero CCL81 None None None None ND ND ND ND
Vero E6
None
None

None
None

ND
ND

ND
ND
7
141
28
38
Vero CCL81 None None None None ND ND ND ND
Vero E6
None
None

None
None

ND
ND

ND
ND
8 165 28 35.0 Vero CCL81 None None None None ND ND ND ND
BHK-21 None None None None ND ND ND ND

*Ct, cycle threshold; CPE, cytopathic effect; NA, not applicable; NCDC, National Centre for Disease Control, New Delhi, India; ND, not done; NIV, National Institute of Virology, Pune, India; RT-PCR, reverse transcription PCR.
†Primers from http://www.crcf.sn/wp-content/uploads/2014/09/CDC-Ebola-International-Lab.pdf.
‡Primers from the Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
§Primers from (7).
¶Samples received at NCDC only.

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References
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  2. World Health Organization. WHO Statement on the Meeting of the International Health Regulations Emergency Committee Regarding the 2014 Ebola Outbreak in West Africa; 2014 Aug 8; Geneva. Geneva: The Organization; 2014 [cited 2014 Nov 3]. http://www.who.int/mediacentre/news/statements/2014/ebola-20140808/en/
  3. Osterholm  MT, Moore  KA, Kelley  NS, Brosseau  LM, Wong  G, Murphy  FA, Transmission of Ebola viruses: what we know and what we do not know. mBiol. 2015;6 [cited 2016 Jan 19]. http://dx.doi: DOI
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  11. Deen  GF, Knust  B, Broutet  N, Sesay  FR, Formenty  P. Ross  C Ebola RNA persistence in semen of Ebola virus disease survivors—preliminary report. N Engl J Med. 2015 Oct 14 [cited 2015 Oct 20]. DOI

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Page created: March 16, 2016
Page updated: March 16, 2016
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