Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 22, Number 6—June 2016
Letter

Next-Generation Sequencing of Mycobacterium tuberculosis

Igor MokrousovComments to Author , Ekaterina Chernyaeva, Anna Vyazovaya, Viacheslav Sinkov, Viacheslav Zhuravlev, and Olga Narvskaya
Author affiliations: St. Petersburg Pasteur Institute, St. Petersburg, Russia (I. Mokrousov, A. Vyazovaya, O. Narvskaya); St. Petersburg State University, St. Petersburg (E. Chernyaeva); Research Institute of Phthisiopulmonology, St. Petersburg (E. Chernyaeva, V. Zhuravlev, O. Narvskaya); Scientific Center of Family Health and Reproductive Problems, Irkutsk, Russia (V. Sinkov

Main Article

Figure

A) Spoligotyping profiles of H37Rv and BCG reference strains, Mycobacterium tuberculosis SIT266 (2 strains in this study) and SIT264 (previously published strain in [3]). SIT, spoligotype international type, designated according to SITVIT_WEB database (http://www.pasteur-guadeloupe.fr:8081/SITVIT_ONLINE). B) Schematic view of the DR/CRISPR locus (region of spacers 7–9) in spoligotypes SIT264 and SIT266 and reference strain H37Rv. Reverse DRa primer is biotin-labeled. IS6110 is not to scale.

Figure. A) Spoligotyping hybridization profiles of H37Rv and BCG reference strains, Mycobacterium tuberculosis SIT266 (2 strains in this study) and SIT264 (previously published strain in [3]). SIT, spoligotype international type, designated according to SITVIT_WEB database (http://www.pasteur-guadeloupe.fr:8081/SITVIT_ONLINE). B) Schematic view of the DR/CRISPR locus (region of spacers 7–9) in spoligotypes SIT264 and SIT266 and reference strain H37Rv, inferred from next-generation sequencing data. Reverse DRa primer is biotin-labeled. IS6110 is not to scale.

Main Article

Page created: May 17, 2016
Page updated: May 17, 2016
Page reviewed: May 17, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external