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Volume 22, Number 6—June 2016
Letter

MERS-CoV Infection of Alpaca in a Region Where MERS-CoV is Endemic

Chantal B.E.M. Reusken1Comments to Author , Chrispijn Schilp1, V. Stalin Raj, Erwin De Bruin, Robert H.G. Kohl, Elmoubasher A.B.A. Farag, Bart L. Haagmans, Hamad Al-Romaihi, Francois Le Grange, Berend-Jan Bosch, and Marion P.G. Koopmans
Author affiliations: Erasmus Medical Center, Rotterdam, the Netherlands (C.B.E.M. Reusken, V.S. Raj, E. De Bruin, R.H.G. Kohl, B.L. Haagmans, M.P.G. Koopmans); Al Wabra Wildlife Preservation, Doha, Qatar (C. Schilp, F. Le Grange); Supreme Council of Health, Doha (E.A.B.A. Farag, H. Al-Romaihi); Utrecht University Faculty of Veterinary Medicine, Utrecht, the Netherlands (B.-J. Bosch)

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Figure

<!-- Q3 -->Column scatterplots of MERS-CoV reactivity of serum samples from alpaca (n = 15) and dromedaries (n = 10) in the Al Shahaniya region of Qatar, April 2015. A) Plot of alpaca and dromedary serum titers of antibodies specific for S1 antigens of 2 coronaviruses as determined by protein microarray. Titers were defined as the interpolated serum concentration that provoked a response half-way on a concentration-response curve between the minimum and maximum signal and were calculated from th

Figure. Column scatterplots of MERS-CoV reactivity of serum samples from alpaca (n = 15) and dromedaries (n = 10) in the Al Shahaniya region of Qatar, April 2015. A) Plot of alpaca and dromedary serum titers of antibodies specific for S1 antigens of 2 coronaviruses as determined by protein microarray. Titers were defined as the interpolated serum concentration that provoked a response half-way on a concentration-response curve between the minimum and maximum signal and were calculated from the inflection point of a 4-step dilution series (1:20 to 1:1,280) as described previously (5). B) Plot of alpaca and dromedary serum titers of MERS-CoV neutralizing antibodies as determined by PRNT90. The highest serum dilution neutralizing 90% of plaque formation is depicted. For both panels, solid lines indicate median, and dotted lines indicate detection limit. MERS, Middle East respiratory syndrome; CoV, coronavirus; PRNT90, 90% plaque-reduction neutralization test; SARS, severe acute respiratory syndrome.

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References
  1. Chan  JF, Lau  SK, To  KK, Cheng  VC, Woo  PC, Yuen  KY. Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease. Clin Microbiol Rev. 2015;28:465522. DOIPubMed
  2. Haagmans  BL, Al Dhahiry  SH, Reusken  CB, Raj  VS, Galiano  M, Myers  R, Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation. Lancet Infect Dis. 2014;14:1405.PubMed
  3. Reusken  CB, Farag  EA, Haagmans  BL, Mohran  KA, Godeke  GJVV, Raj  S, Occupational exposure to dromedaries and risk for MERS-CoV infection, Qatar, 2013–2014. Emerg Infect Dis. 2015;21:14225. DOIPubMed
  4. Reusken  CB, Haagmans  BL, Müller  MA, Gutierrez  C, Godeke  GJ, Meyer  B, Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study. Lancet Infect Dis. 2013;13:85966. DOIPubMed
  5. Koopmans  M, de Bruin  E, Godeke  GJ, Friesema  I, van Gageldonk  R, Schipper  M, Profiling of humoral immune responses to influenza viruses by using protein microarray. Clin Microbiol Infect. 2012;18:797807. DOIPubMed
  6. Corman  VM, Eckerle  I, Bleicker  T, Zaki  A, Landt  O, Eschbach-Bludau  M, Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill. 2012;17:20285 .PubMed
  7. Sabir  JS, Lam  TT, Ahmed  MM, Li  L, Shen  Y, Abo-Aba  SE, Co-circulation of three camel coronavirus species and recombination of MERS-CoVs in Saudi Arabia. Science. 2016;351:814. DOIPubMed
  8. Eckerle  I, Corman  VM, Müller  MA, Lenk  M, Ulrich  RG, Drosten  C. Replicative capacity of MERS coronavirus in livestock cell lines. Emerg Infect Dis. 2014;20:2769 . DOIPubMed
  9. Chan  SM, Damdinjav  B, Perera  RA, Chu  DK, Khishgee  B, Enkhbold  B, Absence of MERS-coronavirus in Bactrian camels, southern Mongolia, November 2014. Emerg Infect Dis. 2015;21:126971. DOIPubMed
  10. Wernery  U, Kinne  J. Foot and mouth disease and similar virus infections in camelids: a review. Rev Sci Tech. 2012;31:90718 .PubMed

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1These authors contributed equally to this article.

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