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Volume 22, Number 6—June 2016
Letter

MERS-CoV Infection of Alpaca in a Region Where MERS-CoV is Endemic

Chantal B.E.M. Reusken1Comments to Author , Chrispijn Schilp1, V. Stalin Raj, Erwin De Bruin, Robert H.G. Kohl, Elmoubasher A.B.A. Farag, Bart L. Haagmans, Hamad Al-Romaihi, Francois Le Grange, Berend-Jan Bosch, and Marion P.G. Koopmans
Author affiliations: Erasmus Medical Center, Rotterdam, the Netherlands (C.B.E.M. Reusken, V.S. Raj, E. De Bruin, R.H.G. Kohl, B.L. Haagmans, M.P.G. Koopmans); Al Wabra Wildlife Preservation, Doha, Qatar (C. Schilp, F. Le Grange); Supreme Council of Health, Doha (E.A.B.A. Farag, H. Al-Romaihi); Utrecht University Faculty of Veterinary Medicine, Utrecht, the Netherlands (B.-J. Bosch)

Main Article

Figure

<!-- Q3 -->Column scatterplots of MERS-CoV reactivity of serum samples from alpaca (n = 15) and dromedaries (n = 10) in the Al Shahaniya region of Qatar, April 2015. A) Plot of alpaca and dromedary serum titers of antibodies specific for S1 antigens of 2 coronaviruses as determined by protein microarray. Titers were defined as the interpolated serum concentration that provoked a response half-way on a concentration-response curve between the minimum and maximum signal and were calculated from th

Figure. Column scatterplots of MERS-CoV reactivity of serum samples from alpaca (n = 15) and dromedaries (n = 10) in the Al Shahaniya region of Qatar, April 2015. A) Plot of alpaca and dromedary serum titers of antibodies specific for S1 antigens of 2 coronaviruses as determined by protein microarray. Titers were defined as the interpolated serum concentration that provoked a response half-way on a concentration-response curve between the minimum and maximum signal and were calculated from the inflection point of a 4-step dilution series (1:20 to 1:1,280) as described previously (5). B) Plot of alpaca and dromedary serum titers of MERS-CoV neutralizing antibodies as determined by PRNT90. The highest serum dilution neutralizing 90% of plaque formation is depicted. For both panels, solid lines indicate median, and dotted lines indicate detection limit. MERS, Middle East respiratory syndrome; CoV, coronavirus; PRNT90, 90% plaque-reduction neutralization test; SARS, severe acute respiratory syndrome.

Main Article

References
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1These authors contributed equally to this article.

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