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Volume 22, Number 7—July 2016
Dispatch

Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Jesse J. Waggoner, Lionel Gresh, Alisha Mohamed-Hadley, Gabriela Ballesteros, Maria Jose Vargas Davila, Yolanda Tellez, Malaya K. Sahoo, Angel Balmaseda, Eva Harris, and Benjamin A. PinskyComments to Author 
Author affiliations: Stanford University School of Medicine, Stanford, California, USA (J.J. Waggoner, A. Mohamed-Hadley, M.K. Sahoo, B.A. Pinsky); Sustainable Sciences Institute, Managua, Nicaragua (L. Gresh); Laboratory Centro Nacional de Diagnóstico y Referencia of the Ministry of Health, Managua (G. Ballesteros, M.J. Vargas Davila, Y. Tellez, A. Balmaseda); School of Public Health of the University of California, Berkeley, Berkeley, California, USA (E. Harris)

Main Article

Table 2

Comparison of Zika virus detection in the ZCD assay and the Zika virus comparator rRT-PCR*

ZCD assay Zika virus rRT-PCR
Pos Neg Total
Pos 25 31† 56
Neg 1‡ 76 77
Total 26 107 133

*CHIKV, chikungunya virus; Ct, cycle threshold; DENV, dengue virus; neg, negative; pos, positive; rRT-PCR, real-time reverse transcription PCR; ZCD assay, single-reaction multiplex rRT-PCR for Zika virus, CHIKV, and DENV.
†22 samples produced a late Ct in the comparator Zika virus rRT-PCR (Ct >38.5)
‡Sample was also pos for a DENV-CHIKV co-infection and tested pos for Zika virus when repeated in the ZCD assay.

Main Article

Page created: June 14, 2016
Page updated: June 14, 2016
Page reviewed: June 14, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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