Volume 22, Number 7—July 2016
Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses
|ZCD assay||Zika virus rRT-PCR
*CHIKV, chikungunya virus; Ct, cycle threshold; DENV, dengue virus; neg, negative; pos, positive; rRT-PCR, real-time reverse transcription PCR; ZCD assay, single-reaction multiplex rRT-PCR for Zika virus, CHIKV, and DENV.
†22 samples produced a late Ct in the comparator Zika virus rRT-PCR (Ct >38.5)
‡Sample was also pos for a DENV-CHIKV co-infection and tested pos for Zika virus when repeated in the ZCD assay.