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Volume 22, Number 9—September 2016
Dispatch

Multidrug-Resistant Escherichia coli in Bovine Animals, Europe

Evan Brennan1, Marta Martins12, Matthew P. McCusker, Juan Wang, Bruno Martins Alves, Daniel Hurley, Farid El Garch, Frédérique Woehrlé, Christine Miossec, Leisha McGrath, Shabarinath Srikumar, Patrick Wall, and Séamus FanningComments to Author 
Author affiliations: University College Dublin, Dublin, Ireland (E. Brennan, M. Martins, M.P. McCusker, J. Wang, B. Martins Alves, D. Hurley, L. McGrath, S. Srikumar, P. Wall, S. Fanning); Northwest A&F University, Yangling, China (J. Wang); Vétoquinol SA, Lure, France (F. El Garch, F. Woehrlé, C. Miossec)

Main Article

Table

Selected characteristics of 3 colistin-resistant Escherichia coli isolates cultured from cattle with suspected enteric or mastitic infections, France and Germany, 2004–2010*

E. coli isolate Year of isolation Phylotype ST Plasmid size, kbp PBRT† Antimicrobial
resistance profile Antimicrobial resistance genotypes† Colisitin MIC, mg/L
22134 2004 U ST10 147; 57; 36 IncFIB, IncFIC, IncFII AML, NAL, CT blaTEM-1B, strAB, tet(34), gyrA, parE, pmrA, pmrB 8
11-1896 2010 U ST58 147; 120; 36; 28; 22; 15; 2 IncFIB, IncFII, IncI1, IncQ1, IncX4 AML, CT, CTX, KF, TE, STX blaCTX-M-1, blaTEM-1B, strAB, sul2, tet(A), tet(34), dfrA5, gyrB, pmrA, pmrB, phoB, eptB 8
29957
 2007
 A or C
 ST167
 200; 147; 36
 IncFIA, IncFIB,
IncFIC,
IncFII,
IncH12,
IncHI2A,
IncQ1
 AMC, AML, CN, CIP, CT, FLO, MAR, NAL, TE, STX
 blaTEM-1A, blaTEM-1B, aadA1, aadA2, aadB, aph(3′)-Ia, aac(3)-Iia, strAB, sul1, sul2, sul3, tet(A), tet(B), tet(34), dfrA1, mcr-1, mef(B), catA1, cmlA1, floR, gyrA, parC, pmrB
 4
*With the exception of mcr-1 in E. coli 29957, genes shown in bold are located on the chromosome in which nonsynonymous amino acid substitutions were identified in the corresponding proteins (online Technical Appendix Tables 1, 2, http://wwwnc.cdc.gov/EID/article/22/9/16-0140-Techapp1.pdf) known to confer resistance to quinolones and colistin. Plasmid replicon types and mcr-1 genes shown in bold were further confirmed by PCR. AMC, amoxicillin/clavulanate; AML, amoxicillin; CN, gentamicin; CT, colistin; CIP, ciprofloxacin; CTX, cefotaxime; FLO, florfenicol; Inc, incompatibility type, KF, cephalothin; NAL, nalidixic acid; MAR, marbofloxacin; PBRT, PCR-based replicon types; TE, tetracycline; ST, sequence type; STX, trimethoprim/ sulfamethoxazole.
†Indicates plasmid replicon types and antimicrobial resistance genotypes extracted from whole genome sequencing data.

Main Article

1These authors contributed equally to this article.

2Current affiliation: Trinity College Dublin, Dublin, Ireland.

Page created: August 16, 2016
Page updated: August 16, 2016
Page reviewed: August 16, 2016
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