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Volume 22, Number 9—September 2016
Letter

mcr-1–Positive Colistin-Resistant Escherichia coli in Traveler Returning to Canada from China

Michael Payne1Comments to Author , Matthew A. Croxen1, Tracy D. Lee, Brian Mayson, Sylvie Champagne, Victor Leung, Sherri Bariso, Linda Hoang, and Christopher Lowe
Author affiliations: Providence Health Care, Vancouver, British Columbia, Canada (M. Payne, B. Mayson, S. Champagne, V. Leung, S. Bariso, C. Lowe); The University of British Columbia, Vancouver (M. Payne, B. Mayson, S. Champagne, V. Leung, S. Bariso, L. Hoang, C. Lowe); British Columbia Centre for Disease Control Public Health Laboratory, Vancouver (M. Croxen, T.D. Lee, L. Hoang)

Main Article

Figure

The mcr-1–containing contig from Escherichia coli isolated from a traveler returning to Canada from China compared to pHNSHP45 plasmid. BRIG (6) was used to generate a visual representation of the 60,599-nt contig assembled from the sequencing of mcr-1 positive isolate to pHNSHP45 (2). Coding regions are represented in the inner ring; the mcr-1–containing contig is represented in the outer ring. The mcr-1 gene is indicated in a black segment in the inner ring.

Figure. The mcr-1–containing contig from Escherichia coli isolated from a traveler returning to Canada from China compared to pHNSHP45 plasmid. BRIG (6) was used to generate a visual representation of the 60,599-nt contig assembled from the sequencing of the mcr-1–positive isolate to pHNSHP45 (2). Coding regions are represented in the inner ring; the mcr-1–containing contig is represented in the outer ring. The mcr-1 gene is indicated in a black segment in the inner ring.

Main Article

References
  1. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 6.0. The Committee; 2016. http://aurosan.de/wp-content/uploads/2015/05/v_6.0_Breakpoint_table.pdf
  2. Liu  YY, Wang  Y, Walsh  TR, Yi  LX, Zhang  R, Spencer  J, Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: A microbiological and molecular biological study. Lancet Infect Dis. 2016;16:161–8.PubMedGoogle Scholar
  3. Ingle  D, Valcanis  M, Kuzevski  A, Tauschek  M, Inouye  M, Stinear  T, In silico serotyping of E. coli from short read data identifies limited novel O-loci but extensive diversity of O:H serotype combinations within and between pathogenic lineages. Microbial Genomics. 2016.
  4. Carattoli  A, Zankari  E, García-Fernández  A, Voldby Larsen  M, Lund  O, Villa  L, In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother. 2014;58:3895–903 .DOIPubMedGoogle Scholar
  5. Bankevich  A, Nurk  S, Antipov  D, Gurevich  AA, Dvorkin  M, Kulikov  AS, SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19:455–77 .DOIPubMedGoogle Scholar
  6. Alikhan  NF, Petty  NK, Ben Zakour  NL, Beatson  SA. BLAST ring image generator (BRIG): simple prokaryote genome comparisons. BMC Genomics. 2011;12:402.
  7. Skov  RL, Monnet  DL. Plasmid-mediated colistin resistance (mcr-1 gene): three months later, the story unfolds. Euro Surveill. 2016;21:30155 .DOIPubMedGoogle Scholar
  8. Hindler  JA, Humphries  RM. Colistin MIC variability by method for contemporary clinical isolates of multidrug-resistant Gram-negative bacilli. J Clin Microbiol. 2013;51:1678–84 .DOIPubMedGoogle Scholar
  9. Mulvey  MR, Mataseje  LF, Robertson  J, Nash  JH, Boerlin  P, Toye  B, Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis. 2016;16:289–90 .DOIPubMedGoogle Scholar
  10. Poirel  L, Kieffer  N, Liassine  N, Thanh  D, Nordmann  P. Plasmid-mediated carbapenem and colistin resistance in a clinical isolate of Escherichia coli. Lancet Infect Dis. 2016;16:281 .DOIPubMedGoogle Scholar

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1These authors contributed equally to this article.

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