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Volume 22, Number 9—September 2016
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Changing Diagnostic Methods and Increased Detection of Verotoxigenic Escherichia coli, Ireland

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Thomas Rice, Noreen Quinn, Roy D. SleatorComments to Author , and Brigid Lucey
Author affiliations: Cork Institute of Technology School of Science and Informatics, Bishopstown, Cork, Ireland

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Abstract

The recent paradigm shift in infectious disease diagnosis from culture-based to molecular-based approaches is exemplified in the findings of a national study assessing the detection of verotoxigenic Escherichia coli infections in Ireland. The methodologic changes have been accompanied by a dramatic increase in detections of non-O157 verotoxigenic E. coli serotypes.

Verotoxigenic Escherichia coli (VTEC) can cause severe disease in humans, with signs and symptoms including diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (1). The primary reservoir of VTEC is ruminants, and sporadic outbreaks are commonly associated with animal contact, exposure to animal feces, food and water contamination, and person-to-person transmission (2).

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Thumbnail of Number of reported cases of O157 and non-O157 VTEC infection and number of laboratories performing PCR-based detection of stx gene, by year, Ireland, 2010–2014. stx, Shiga toxin; VTEC, verotoxigenic Escherichia coli.

Figure. Number of reported cases of O157 and non-O157 VTEC infection and number of laboratories performing PCR-based detection of stx gene, by year, Ireland, 2010–2014. stx, Shiga toxin; VTEC, verotoxigenic Escherichia coli.

In Ireland, a country with a population of ≈4.6 million, all VTEC infections have been notifiable to the Medical Officer of Health since 2004. Initially, these infections were included under specified infections caused by enterohemorrhagic E. coli (EHEC), comprising any strains of serogroups O157, O26, O103, O111, and O145 (3); since an amendment of September 2011, these infections have been specified as VTEC infections (4). Data on VTEC infections in Europe have been provided since 2008 from the member states to the European Centre for Disease Prevention and Control. These data indicate that Ireland had the highest annual incidence of VTEC infection during 2009–2014, with the exception of 2011, when a large outbreak of E. coli O104:H4 infections occurred in Germany (5). The Health Protection and Surveillance Centre releases annual reports on the number of VTEC infection cases in Ireland, which document an increase in the number of VTEC infections in Ireland during 2010–2014 (Figure). This trend is thought to be largely attributable to an increase in non-O157 VTEC infections. A low infectious dose of ≈10 microorganisms has been described as sufficient to cause disease that can lead to hemolytic uremic syndrome (6). Real-time PCR (rPCR) has been demonstrated as a more rapid and sensitive method of VTEC detection, compared with enzyme immunoassay and culture (7).

We conducted a survey of clinical microbiology laboratories in Ireland during 2014 to assess laboratory practices for the detection and confirmation of VTEC infection. The survey included 45 questions, arranged in 4 categories: laboratory details (e.g., contact details, address, and public/private status); sample type, selection, and requirements for VTEC testing; current methods (i.e., utilization and description of enrichment, culture, biochemical characterization, antimicrobial susceptibility testing, serotyping, molecular testing, and verotoxin detection methods), past changes to methods, and intended future changes to methods for VTEC analyses; and referral and reporting procedures. Ireland is home to 25 clinical microbiology laboratories, all of which were represented in the survey findings presented here. Laboratories included the VTEC National Reference Laboratory in Cherry Orchard Hospital, Dublin, which provides the referral service for this pathogen to all clinical laboratories in Ireland, whether public or private. Laboratories were categorized into 3 groups based on their testing strategy: culture-based detection, PCR-based detection, or both. An analysis of the introduction of molecular-based detection of VTEC infection (i.e., rPCR of verotoxin genes vt1 and vt2) in clinical laboratories in Ireland indicated a timeline for the implementation of these methods from 2010 to 2014. Before 2012, the reference laboratory was the sole laboratory implementing PCR-based detection of VTEC. In 2012, a regional clinical laboratory introduced an automated molecular platform for the detection of VTEC and other gastrointestinal pathogens. Four more laboratories introduced this molecular platform in 2013, with another 2 converting in 2014. Five clinical laboratories expressed an intention to introduce PCR-based detection of VTEC starting in 2015.

These data represent a shifting trend from the conventional culture-based detection of fecal coliforms (with confirmatory testing as VTEC), to a direct molecular approach in which stool specimens are tested initially for the presence of the verotoxin genes. This move toward molecular detection of VTEC infection is consistent with the increased number of non-O157 VTEC cases reported in Ireland in recent years (Figure). Similar results were reported in a study of the same nature conducted in Washington, USA (8). Along with the increased number of clinical laboratories participating in molecular detection of VTEC, a concomitant shift has occurred in the culture-based detection of VTEC. The introduction of an agar for the detection of Shiga-toxin–producing E. coli (CHROMagar STEC; Mast Diagnostika, Reinfeld, Germany) to laboratories, which is selective for the top 6 non-O157 serogroups (O26, O45, O103, O111, O121, and O145), supports the recently held view that a range of VTEC serogroups are now considered a major cause of human VTEC infection (5,9).

With the exception of the reference laboratory, molecular VTEC detection in clinical laboratories in Ireland use a commercial rPCR-based platform. This method, which selects for verotoxin genes, accelerates more sensitive detection compared with traditional culture-based methods.

Our own recent observations in Ireland (10,11) may very well represent the start of a global trend toward molecular detection of verotoxin genes from clinical and other samples, to replace culture-based methods, as a first step in the detection of VTEC (12). Our findings suggest that increases in notifications of VTEC infections, and particularly those caused by non-O157 serotypes, be interpreted in light of this paradigm shift in infectious disease diagnostic methods.

Mr. Rice is a medical scientist who is currently undertaking a PhD at Cork Institute of Technology and University College, Cork, jointly.

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Acknowledgments

We wish to thank Anne Carroll, Brendan O’Reilly, and Patrick Mulhare.

This study was made possible by financial support from the Food Safety Promotion Board of Ireland (safefood).

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References

  1. Page  AV, Liles  WC. Enterohemorrhagic Escherichia coli infections and the hemolytic-uremic syndrome. Med Clin North Am. 2013;97:68195. DOIPubMed
  2. Snedeker  KG, Shaw  DJ, Locking  ME, Prescott  RJ. Primary and secondary cases in Escherichia coli O157 outbreaks: a statistical analysis. BMC Infect Dis. 2009;9:144. DOIPubMed
  3. No  SI. 707/2003: infectious diseases (amendment) (no. 3) regulations 2003, Ireland [cited 2016 Jun 13]. http://www.irishstatutebook.ie/eli/2003/si/707/made/en/print
  4. No  SI. 452/2011: infectious diseases (amendment) regulations 2011, Ireland [cited 2016 Jun 13]. http://www.irishstatutebook.ie/eli/2011/si/452/made/en/print
  5. European Food Safety Authority, European Centre for Disease Prevention and Control. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013. European Food Safety Authority Journal. 2015;13:4329. DOI
  6. Vallières  E, Saint-Jean  M, Rallu  F. Comparison of three different methods for detection of Shiga toxin-producing Escherichia coli in a tertiary pediatric care center. J Clin Microbiol. 2013;51:4816. DOIPubMed
  7. Grys  TE, Sloan  LM, Rosenblatt  JE, Patel  R. Rapid and sensitive detection of Shiga toxin-producing Escherichia coli from nonenriched stool specimens by real-time PCR in comparison to enzyme immunoassay and culture. J Clin Microbiol. 2009;47:200812. DOIPubMed
  8. Stigi  KA, Macdonald  JK, Tellez-Marfin  AA, Lofy  KH. Laboratory practices and incidence of non-O157 Shiga toxin-producing Escherichia coli infections. Emerg Infect Dis. 2012;18:4779 .DOIPubMed
  9. Gould  LH, Bopp  C, Strockbine  N, Atkinson  R, Baselski  V, Body  B, Recommendations for diagnosis of Shiga toxin–producing Escherichia coli infections by clinical laboratories. MMWR Recomm Rep. 2009;58(RR-12):114.PubMed
  10. O’Leary  J, Corcoran  D, Lucey  B. Comparison of the EntericBio multiplex PCR system with routine culture for detection of bacterial enteric pathogens. J Clin Microbiol. 2009;47:344953. DOIPubMed
  11. Koziel  M, Corcoran  D, O’Callaghan  I, Sleator  RD, Lucey  B. Validation of the EntericBio Panel II® multiplex polymerase chain reaction system for detection of Campylobacter spp., Salmonella spp., Shigella spp., and verotoxigenic E. coli for use in a clinical diagnostic setting. Diagn Microbiol Infect Dis. 2013;75:469. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=23182078&dopt=AbstractDOIPubMed
  12. Mingle  LA, Garcia  DL, Root  TP, Halse  TA, Quinlan  TM, Armstrong  LR, Enhanced identification and characterization of non-O157 Shiga toxin-producing Escherichia coli: a six-year study. Foodborne Pathog Dis. 2012;9:102836 .DOIPubMed

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Cite This Article

DOI: 10.3201/eid2209.160477

Table of Contents – Volume 22, Number 9—September 2016

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Roy D. Sleator, Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork T12 P928, Ireland

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Page created: August 16, 2016
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