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Volume 23, Number 2—February 2017

Cerebrospinal Fluid Findings in an Adult with Human Metapneumovirus–Associated Encephalitis

Natalie Jeannet, Bernadette G. van den Hoogen, Joerg C. Schefold, Franziska Suter-Riniker, and Rami SommersteinComments to Author 
Author affiliations: University of Bern, Bern, Switzerland (N. Jeannet, J.C. Schefold, F. Suter-Riniker, R. Sommerstein); Erasmus Medical Center, Rotterdam, the Netherlands (B.G. van den Hoogen); Bern University Hospital, Bern (J.C. Schefold, R. Sommerstein)

Main Article


Results of PCR and immunofluorescent assay testing in adult patient with HMPV*

Sample type, result or IgG titer
HMPV IgG index
Nasopharyngeal swab
Reverse transcription PCR
Positive (cycle threshold 28.6)

Immunofluorescence assays
Vero-118 cells infected with HMPV NL/1/00 1:8,192 1:32 0.54
Vero-118 cells infected with HMPV NL/1/99 1:8,192 1:64 1.07

*A duplex reverse transcription PCR (r-gene) for detection of human metapneumovirus (HMPV) was performed from a nasopharyngeal swab specimen and cerebrospinal fluid (CSF). For immunofluorescence assays, 96-well plates coated with Vero 118 cells were infected with HMPV NL/1/00 and NL/1/99, respectively. Twenty-four hours later, infected cells were incubated with serial dilutions of patient serum and CSF for 1 h at 37°C. After washing with phosphate-buffered saline, plates were incubated with anti-human IgG conjugated with fluorescein isothiocyanate for 1 h at 37°C. Lowest dilution giving a positive result was determined by UV microscopy. Intrathecal IgG synthesis was calculated using the formula (IgGCSF HMPV/IgGSerum HMPV)/(IgGCSF total/IgGSerum total). Indices below 4 indicate absence of intrathecal IgG antibody synthesis.

Main Article

Page created: January 18, 2017
Page updated: January 18, 2017
Page reviewed: January 18, 2017
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