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Volume 23, Number 3—March 2017
Research

New Mycobacterium tuberculosis Complex Sublineage, Brazzaville, Congo

Sven Malm, Laure S. Ghoma Linguissi, Emmanuel M. Tekwu, Jeannhey C. Vouvoungui, Thomas A. Kohl, Patrick Beckert, Anissa Sidibe, Sabine Rüsch-Gerdes, Igor K. Madzou-Laboum, Sylvie Kwedi, Véronique Penlap Beng, Matthias Frank, Francine Ntoumi, and Stefan NiemannComments to Author 
Author affiliations: Leibniz Center for Medicine and Biosciences, Borstel, Germany (S. Malm, T.A. Kohl, P. Beckert, S. Rüsch-Gerdes, S. Niemann); Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo (L.S. Ghoma Linguissi, J.C. Vouvoungui, A. Sidibe, S. Kwedi, F. Ntoumi); University Marien Ngouabi, Brazzaville (L.S. Ghoma Linguissi, J.C. Vouvoungui, A. Sidibe, F. Ntoumi); University of Yaoundé I, Yaoundé, Cameroon (E.M. Tekwu, V. Penlap Beng); Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany (E.M. Tekwu, M. Frank, F. Ntoumi); Centre Antituberculeux de Brazzaville, Brazzaville (I.K. Madzou-Laboum); German Center for Infection Research, Tübingen Site, Tübingen (M. Frank); German Center for Infection Research, Borstel Site, Borstel (S. Niemann)

Main Article

Figure 1

Maximum-likelihood tree of Mycobacterium tuberculosis complex isolates from Congo (black branch tips) and 65 reference strains (red branch tips). The tree was calculated by using the general time reversible substitution model with gamma distribution based on single-nucleotide polymorphisms identified by whole-genome sequencing. Models were tested and the tree generated by using MetaPiga software version 3.1 (18) and the maximum-likelihood ratio test. Midpoint rooting was performed. Distinct colo

Figure 1. Maximum-likelihood tree of Mycobacterium tuberculosis complex isolates from Congo (black branch tips) and 65 reference strains (red branch tips). The tree was calculated by using the general time reversible substitution model with gamma distribution based on single-nucleotide polymorphisms identified by whole-genome sequencing. Models were tested and the tree generated by using MetaPiga software version 3.1 (18) and the maximum-likelihood ratio test. Midpoint rooting was performed. Distinct colors were chosen for the lineages identified; leaves with white background represent strains that initially were not assigned to particular lineages because of ambiguous typing patterns from mycobacterial interspersed repetitive unit, restriction fragment length polymorphism, or spoligo analysis (data not shown). The numerical code assigned to the respective lineages at the outer rim of the circular tree shows the Coll-nomenclature inferred from the whole-genome sequencing data. EAI, East African Indian; LAM, Latin American Mediterranean; TUR, Turkish. Scale bar indicates nucleotide substitutions per site.

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Page created: February 17, 2017
Page updated: February 17, 2017
Page reviewed: February 17, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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