*del, deletion; ins, insertion; MDR, multidrug resistant; RFLP, restriction fragment-length polymorphism; SNP, single-nucleotide polymorphism; TB, tuberculosis; XDR, extensively drug-resistant. Row colors: pink, frameshift mutations (insertions or deletions); green, synonymous mutations or SNPs reported as being associated with phenotypic susceptibility to pyrazinamide; gray, SNPs reported as being associated with phenotypic resistance to pyrazinamide; blue, single-nucleotide polymorphisms not previously reported in the literature.
†Insertions and deletions are presented with the nucleotide position where the polymorphism occurred; SNPs are presented with the codon position and the original and mutated 3 nucleotides and amino acid.
‡The nomenclature used for classifying IS6110 RFLP patterns was as follows: 2 isolates with an identical IS6110 banding pattern were assigned the same arbitrary 1- or 2-letter code (e.g., W, HP, or AB), which started with the first observed cluster, strain A (several decades ago). IS6110 patterns that were similar but not identical were denoted by the addition of a number (e.g., BE1, W4, or HP81). This table shows only the letter designations; numbers have been omitted for simplicity. Strains within each of the 2-letter designations had similar RFLP patterns.
§Reported in the literature as being associated with resistance to pyrazinamide.
¶Synonymous mutation.
#Reported in the literature as being associated with susceptibility to pyrazinamide.