, Shamez N. Ladhani, Georgia Kapatai, Ella Campion, Norman K. Fry, and Carmen Sheppard
Figure 2. Neighbor-joining tree following single-nucleotide polymorphism analyses on 59 pneumococcal isolates collected in England and Wales during July 2005–June 2017. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branch junctions. All positions with <90% site coverage were eliminated. Missing data and ambiguous bases were allowed at any position. There were a total of 20,359 variant positions in the final dataset. Red nodes represent ST177 isolates; green node, an isolate with SLV of ST177; black nodes, non-ST177 isolates. Squares represent 7C serotype; triangles, 24F serotype; and circles 19F serotype. The open circle represents the Taiwan 19F-14 reference sequence (GenBank accession no. NC_012469.1). Scale bar shows the number of nucleotide substitutions represented by branch length. ST, sequence type.