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Volume 24, Number 10—October 2018

Research Letter

Community-Acquired Staphylococcus argenteus Sequence Type 2250 Bone and Joint Infection, France, 2017

Josselin Rigaill, Florence Grattard, Sylvain Grange, Fabien Forest, Elie Haddad, Anne Carricajo, Anne Tristan, Frederic Laurent, Elisabeth Botelho-NeversComments to Author , and Paul O. Verhoeven
Author affiliations: University Hospital of Saint-Etienne, Saint-Etienne, France (J. Rigaill, F. Grattard, S. Grange, F. Forest, E. Haddad, A. Carricajo, E. Botelho-Nevers, P.O. Verhoeven); Jean Monnet University, Saint-Etienne (J. Rigaill, F. Grattard, A. Carricajo, E. Botelho-Nevers, P.O. Verhoeven); International Centre for Infectiology Research, Lyon, France (A. Tristan, F. Laurent); French National Reference Centre for Staphylococci, Lyon (A.Tristan, F. Laurent)

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EID Rigaill J, Grattard F, Grange S, Forest F, Haddad E, Carricajo A, et al. Community-Acquired Staphylococcus argenteus Sequence Type 2250 Bone and Joint Infection, France, 2017. Emerg Infect Dis. 2018;24(10):1958-1961. https://dx.doi.org/10.3201/eid2410.180727
AMA Rigaill J, Grattard F, Grange S, et al. Community-Acquired Staphylococcus argenteus Sequence Type 2250 Bone and Joint Infection, France, 2017. Emerging Infectious Diseases. 2018;24(10):1958-1961. doi:10.3201/eid2410.180727.
APA Rigaill, J., Grattard, F., Grange, S., Forest, F., Haddad, E., Carricajo, A....Verhoeven, P. O. (2018). Community-Acquired Staphylococcus argenteus Sequence Type 2250 Bone and Joint Infection, France, 2017. Emerging Infectious Diseases, 24(10), 1958-1961. https://dx.doi.org/10.3201/eid2410.180727.

Abstract

We report a rare case of Staphylococcus argenteus bone and joint infection in a 9-year-old boy in France. His finger arthritis was complicated by osteitis 5 weeks later, which resulted in a secondary intervention. This case indicates the virulence of S. argenteus, an emerging pathogen whose clinical effects are poorly described.

Staphylococcus argenteus (formerly S. aureus clonal complex 75) is an emerging species in the S. aureus complex (1). Several studies reported sporadic cases of S. argenteus infections mainly in Asia, Oceania, and the Pacific Islands (2) but rarely in Europe (3). We report the clinical characteristics of a community-acquired bone and joint infection with S. argenteus in a child living in France.

At the end of July 2017, a 9-year-old boy with no unusual medical history or previous local trauma was hospitalized because of acute signs of infection of the third finger on his right hand. He was first seen in a local hospital and given an initial diagnosis of cellulitis (arthritis). Two days later, he was admitted to the emergency pediatric ward of a tertiary care hospital where a surgical joint exploration was performed and confirmed the diagnosis of arthritis associated with an abscess of the extensor tendon sheath (Table).

Surgical microbiological samples cultured on blood agar plates (aerobic conditions at 37°C for 24 h) grew a strain that was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Microflex LT, Bruker, France) as having log scores ranging from 1.39 to 1.87 (corresponding to S. aureus, S. simiae, or S. epidermidis). These scores were lower than those required for reliable identification of species (2.3) or genus (2.1) levels. Antimicrobial drug susceptibility testing (Vitek2; bioMérieux, Marcy l’Etoile, France) identified resistance to penicillin G.

Because there was no initial reliable identification of this strain, we performed molecular tests. The strain was negative for nuc, lukS-PV, mecA, mecC, tst-1, and spa genes. The V3 region sequence of the gene coding for 16S rRNA showed 100% identity with that for S. aureus. Microarray analysis (S. aureus Genotyping Kit 2.0; Alere Technologies GmbH, Jena, Germany) assigned this strain to the clonal complex 2250/2277 of one of the main clusters of S. argenteus (2,4). The patient was discharged and received an oral antimicrobial regimen for 6 weeks; healing was closely monitored (Table).

Five weeks later, the patient was hospitalized because of recurrent signs of infection (Table). Magnetic resonance imaging of the right hand showed osteitis (Technical Appendix, panel A). A second surgical procedure was performed (Table). Cultures of surgical samples remained sterile after 10 days and a 16S rDNA PCR result was negative. Histologic analysis showed chronic osteitis (Technical Appendix Figure, panel B). An oral drug regimen, including clindamycin and ofloxacin for 6 weeks, was prescribed. Long-term outcome was good (Table), despite persistence of stiffness in the finger.

This rare case of osteomyelitis caused by S. argenteus highlights the difficulties in correctly identifying this species. As with S. schweitzeri, S. argenteus is an emergent species that has been described as part of the S. aureus species complex (5). S. argenteus was first described in 2002 as a CC75/sequence type T1223 clone of S. aureus in Aboriginal communities in Australia (6). This species was named S. argenteus in 2011 because of its lack of staphyloxanthin production (1). Most studies reported prevalence rates for S. argenteus among strain collections of 0.16%–18.6% according to geographic distribution, with a clear predominance in Asia and the West Pacific region (1,57) and a rare description in Europe (3).

In our case, no epidemiologic link to Asia or the West Pacific region was observed. The clinical spectrum of infections with S. argenteus remains poorly described, but varies from asymptomatic nasal carriage (8) to community-acquired infections, including skin and soft tissue infections (1,6), bacteremia (1,9), and foodborne illness (8). Apart from 2 cases of bacteremia reported by Dupieux et al. (9), clinical data for S. argenteus infections has been poorly detailed (Technical Appendix, Figure). To the best of our knowledge, only 1 case of osteomyelitis has been reported (7).

Previous cases and the case we report indicate that S. argenteus could be responsible for invasive infections that are difficult to manage. S. argenteus was initially considered to be less virulent than S. aureus on the basis of a study in Australia, which reported that this species was associated mainly with skin and soft tissue infections and rarely with bacteremia (3/220 cases) (1). However, a comparative study of 311 cases of S. argenteus and S. aureus sepsis in Thailand showed a similar outcome after 28 days (10). Moreover, virulence factors, such as Panton-Valentine leucocidin and enterotoxins, have been described in S. argenteus isolates (4,810). In contrast to reports from Aboriginal communities in Australia (6) and remote populations in the West Pacific region (Technical Appendix, Table), resistance to methicillin was not detected in strains from the case-patients in this study.

In contrast to S. aureus, the effect of carriage of S. argenteus has not been studied. For our case-patient, screening for S. argenteus nasal carriage was not performed. However, a recent study of foodborne illness outbreaks reported the ability of this bacterium to spread in the environment and colonize food handlers (8).

S. argenteus is an emerging species for which its clinical spectrum remains poorly described. Further studies are needed to better address the global prevalence and clinical role of this bacterium, including its potential effects in chronic human infections.

Dr. Rigaill is a microbiology fellow at the University Hospital of Saint-Etienne, Saint-Etienne, France. His primary research interest is identifying the determinants of S. aureus carriage.

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Acknowledgments

We thank the parents of the boy for providing written consent to report the case; the technical staff of the Laboratory of Infectious Agents and Hygiene of the University Hospital of Saint-Etienne and the National Reference Centre for Staphylococci, Lyon, France for assistance; and Philippe Michelucci for English editing of the manuscript.

J.R., S.G., and F.F. performed clinical imaging and collected histologic data; E.H. performed surgery; F.G., A.C., and A.T. analyzed the bacterial strain; and F.L., E.B.-N., and P.O.V. critically revised the manuscript and made final corrections. All authors approved the final manuscript.

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References

  1. Tong  SY, Sharma-Kuinkel  BK, Thaden  JT, Whitney  AR, Yang  S-J, Mishra  NN, et al. Virulence of endemic nonpigmented northern Australian Staphylococcus aureus clone (clonal complex 75, S. argenteus) is not augmented by staphyloxanthin. J Infect Dis. 2013;208:5207. DOIPubMed
  2. Moradigaravand  D, Jamrozy  D, Mostowy  R, Anderson  A, Nickerson  EK, Thaipadungpanit  J, et al. Evolution of the Staphylococcus argenteus ST2250 clone in northeastern Thailand is linked with the acquisition of livestock-associated staphylococcal genes. MBio. 2017;8:e0080217. DOIPubMed
  3. Argudín  MA, Dodémont  M, Vandendriessche  S, Rottiers  S, Tribes  C, Roisin  S, et al. Low occurrence of the new species Staphylococcus argenteus in a Staphylococcus aureus collection of human isolates from Belgium. Eur J Clin Microbiol Infect Dis. 2016;35:101722. DOIPubMed
  4. Wakabayashi  Y, Umeda  K, Yonogi  S, Nakamura  H, Yamamoto  K, Kumeda  Y, et al. Staphylococcal food poisoning caused by Staphylococcus argenteus harboring staphylococcal enterotoxin genes. Int J Food Microbiol. 2018;265:239. DOIPubMed
  5. Tong  SY, Schaumburg  F, Ellington  MJ, Corander  J, Pichon  B, Leendertz  F, et al. Novel staphylococcal species that form part of a Staphylococcus aureus-related complex: the non-pigmented Staphylococcus argenteus sp. nov. and the non-human primate-associated Staphylococcus schweitzeri sp. nov. Int J Syst Evol Microbiol. 2015;65:1522. DOIPubMed
  6. McDonald  M, Dougall  A, Holt  D, Huygens  F, Oppedisano  F, Giffard  PM, et al. Use of a single-nucleotide polymorphism genotyping system to demonstrate the unique epidemiology of methicillin-resistant Staphylococcus aureus in remote aboriginal communities. J Clin Microbiol. 2006;44:37207. DOIPubMed
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  8. Aung  MS, San  T, Aye  MM, Mya  S, Maw  WW, Zan  KN, et al. Prevalence and genetic characteristics of Staphylococcus aureus and Staphylococcus argenteus isolates harboring Panton-Valentine leukocidin, enterotoxins, and TSST-1 genes from food handlers in Myanmar. Toxins (Basel). 2017;9:E241. DOIPubMed
  9. Dupieux  C, Blondé  R, Bouchiat  C, Meugnier  H, Bes  M, Laurent  S, et al. Community-acquired infections due to Staphylococcus argenteus lineage isolates harbouring the Panton-Valentine leucocidin, France, 2014. Euro Surveill. 2015;20:21154. DOIPubMed
  10. Chantratita  N, Wikraiphat  C, Tandhavanant  S, Wongsuvan  G, Ariyaprasert  P, Suntornsut  P, et al. Comparison of community-onset Staphylococcus argenteus and Staphylococcus aureus sepsis in Thailand: a prospective multicentre observational study. Clin Microbiol Infect. 2016;22:458.e119. DOIPubMed

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Cite This Article

DOI: 10.3201/eid2410.180727

Original Publication Date: 9/4/2018

Table of Contents – Volume 24, Number 10—October 2018

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Elisabeth Botelho-Nevers, Infectious Diseases Department, University Hospital of Saint-Etienne, Av Albert Raimond, 42055 Saint-Etienne CEDEX 02, France


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