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Volume 24, Number 12—December 2018
Research

Capnocytophaga canimorsus Capsular Serovar and Disease Severity, Helsinki Hospital District, Finland, 2000–2017

Estelle Hess1, Francesco Renzi1, Panu Karhunen1, Mélanie Dol, Adrien Lefèvre, Jenni Antikainen, Elodie Carlier, Johanna Hästbacka2, and Guy R. Cornelis2Comments to Author 
Author affiliations: University of Namur, Namur, Belgium (E. Hess, F. Renzi, M. Dol, A. Lefèvre, E. Carlier, G.R. Cornelis); University of Eastern Finland, Kuopio, Finland (P. Karhunen); University of Helsinki and Helsinki University Hospital, Helsinki, Finland (J. Antikainen, J. Hästbacka)

Main Article

Table 1

Capsular typing of 73 Capnocytophaga canimorsus isolates from patient blood samples, Helsinki Hospital District, Finland, 2000–2017*

Isolates
 PCR typing†
Western blot typing‡ Serovar
ABC
 A
 B
 C
 D
 E
 A
 B
 C
 D
 E
 F
 G
 H
 I
H11, H16, H23, H37, H39, H42, H48, H52, H56, H60, H62, H70, H74, H75, H76, H78, H80 + + + – – – + – – ND ND ND ND ND ND A
H3, H4, H5, H6, H9, H14, H22, H25, H26, H30, H35, H38, H49, H50, H53, H55, H57, H58, H63, H65, H67, H68, H69, H71, H72, H73, H79 + – + – – – – + – ND ND ND ND ND ND B
H27 + – + – – – + + – ND ND ND ND ND ND B
H1, H7, H8, H10, H13, H15, H17, H18, H19, H20, H28, H29, H33, H34, H36, H43, H44, H45, H46, H47, H51, H59 + – – + – – – – + ND ND ND ND ND ND C
H41, H64 – – – – + – – – – + ND ND ND ND ND D
H31 – – – – – + – – – ND + ND ND ND ND E
H21 – – – – – – – – – – – – – – + I
H12, H24 – – – – – – – – – – – – – – – NT

*ND, not done; NT, nontypeable.
†We performed PCR capsular typing using the oligonucleotides given in Technical Appendix Table). Results were interpreted as done previously (7): isolates positive for PCR ABC, A, and B were typed as A, isolates positive for PCR ABC and B were typed as B, and isolates positive for PCR ABC and C were typed as C.
‡Western blot analyses on polysaccharidic structures were performed by using specific polyclonal rabbit antisera.

Main Article

References
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Main Article

1These first authors contributed equally to this article.

2These authors were co–principal investigators.

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