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Volume 24, Number 12—December 2018
Dispatch

Using PCR-Based Sequencing to Diagnose Haycocknema perplexum Infection in Human Myositis Case, Australia

Anson V. Koehler, Peter Leung, Belinda McEwan, and Robin B. GasserComments to Author 
Author affiliations: University of Melbourne, Parkville, Victoria, Australia (A.V. Koehler, R.B. Gasser); Royal Hobart Hospital, Hobart, Tasmania, Australia (P. Leung, B. McEwan)

Main Article

Table 1

Primer sequences for nested PCR to detect Haycocknema perplexum nematodes using partial regions of the cox-1 gene and the SSU gene*

Designation Primer pair Oligonucleotide sequence, 5′ → 3′ Annealing temperature, °C Expected size, bp Reference
cox-1
1° PCR RhigoCoxF TTTTTTGGACATCCTGAGGTGTAT (3)
RhigoCoxR CAGACTCAACACATAATGAAAATG 47 450 (3)
2° PCR HPCO1F GGACATCCTGAGGTGTATAT This study

HPCO1R
AATGAAAATGTCCTACCACA
50
400
This study
SSU
1° PCR Nem18sF CGCGAATRGCTCATTACAACAGC (1)
1912R TTTACGGTCAGAACTAGGG 54 900 (2)
2° PCR 1096F GGTAATTCTGGAGCTAATAC (2)
Nem18sR GGGCGGTATCTGATCGCC 54 830 (1)

*All PCR used 35 cycles with initial extension of 5 min. All cycles were 30 s except that SSU had a 1 min extension for PCRs. cox-1, cytochrome c oxidase 1; SSU, small subunit of nuclear ribosomal RNA.

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Page created: November 20, 2018
Page updated: November 20, 2018
Page reviewed: November 20, 2018
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