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Volume 24, Number 4—April 2018
Dispatch

Lyssavirus in Japanese Pipistrelle, Taiwan

Shu-Chia Hu, Chao-Lung Hsu, Ming-Shiuh Lee, Yang-Chang Tu, Jen-Chieh Chang, Chieh-Hao Wu, Shu-Hwae Lee, Lu-Jen Ting, Kwok-Rong Tsai, Ming-Chu Cheng, Wen-Jane Tu, and Wei-Cheng HsuComments to Author 
Author affiliations: Animal Health Research Institute, New Taipei City, Taiwan (S.-C. Hu, M.-S. Lee, Y.-C. Tu, J.-C. Chang, C.-H. Wu, L.-J. Ting, K.-R. Tsai, W.-J. Tu, W.-C. Hsu); National Taiwan University, Taipei City, Taiwan (C.-L. Hsu); Bat Conservation Society of Taipei, Taipei City (C.-L. Hsu); Animal Health Research Institute, Miaoli County, Taiwan (S.-H. Lee); National Pingtung University of Science and Technology, Neipu Township, Pingtung County, Taiwan (M.-C. Cheng)

Main Article

Table 1

Lyssavirus screen primers and the 12 amplifying primer sets used to identify Taiwan bat lyssavirus, a putative new lyssavirus found in 2 Japanese pipistrelles (Pipistrellus abramus) in Taiwan in 2016 and 2017

Primer name Sequence, 5′ → 3′ Position*
Lyssavirus screen
JW12 (7) ATGTAACACCYCTACAATG
N165–146 (7) GCAGGGTAYTTRTACTCATA
N113F (8) GTAGGATGCTATATGGG
N304R (9)
 TTGACGAAGATCTTGCTCAT
Lyssavirus full genome
TWBLV 1F ACGCTTAACGACAAAAYC 1–18*
TWBLV 1R TCTTGCATTTCTTTCTCATC 1154–1173
TWBLV 2F TTCGTAGGATGTTACATGGG 1010–1029
TWBLV 2R taaaaatatcccagaagatc 2174–2193
TWBLV 3F AGARATAGCWCATCAGATWGC 2118–2138
TWBLV 3R CTATTGTGTGGCACCATWAC 3205–3224
TWBLV 4F GATGAGGATAAGAACACATC 3072–3091
TWBLV 4R TCCTGAAGTGACTGAGTTTTC 4274–4294
TWBLV 5F CTGATGGAYGGRTCATGGGT 4081–4100
TWBLV 5R GAGACAGGAGCCGGAGTCTT 5280–5299
TWBLV 6F AACAGGTAGCTCCCGAGTTTGTTC 4881–4904
TWBLV 6R CTGAGTGAGACCCATGTATCCAAA 5767–5790
TWBLV 7F ACTGAGGTTTATGATGACCC 5478–5497
TWBLV 7R CCCCAGTGTCTATARCAWCC 6561–6580
TWBLV 8F CATTCTTTGGGGGATTTCCC 6426–6445
TWBLV 8R GTTTGTGATTCTCTRTCWATC 7595–7615
TWBLV 9F CATGCTGGAACGGTCAGGAYG 7522–7542
TWBLV 9R CTGAGTTAAAGAAAGATTCTT 8662–8682
TWBLV 10F CTCAGTGAGTTRTTYAGCTC 8547–8566
TWBLV 10R CAGATAGAAGAGCCTATT 9746–9763
TWBLV 11F CATGATTCAGGGTAYAAYGA 9648–9667
TWBLV 11R GTCTGTAACTTCTGCATCAC 10862–10871
TWBLV 12F ATCTGGGAAAAGCCATCAGA 10755–10774
TWBLV 12R ACGCTTAACAAAAAAAACAA 11961–11980

*Compared with reference genomic sequence, Irkut lyssavirus (GenBank accession no. NC020809). TWBLV, Taiwan bat lyssavirus.

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Page updated: March 19, 2018
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