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Volume 24, Number 6—June 2018
Research Letter

Zooanthroponotic Transmission of Drug-Resistant Pseudomonas aeruginosa, Brazil

Miriam R. Fernandes1, Fábio P. Sellera1, Quézia Moura, Marcelo P.N. Carvalho, Paula N. Rosato, Louise Cerdeira, and Nilton LincopanComments to Author 
Author affiliations: Universidade de São Paulo, São Paulo, Brazil (M.R. Fernandes, F.P. Sellera, Q. Moura, M.P.N. Carvalho, L. Cerdeira, N. Lincopan); Centro Universitário Monte Serrat, Santos, São Paulo (P.N. Rosato)

Main Article

Table

Characteristics of carbapenem-resistant VIM-2 metallo-β-lactamase–producing Pseudomonas aeruginosa isolates in a human–animal–environment interface in a household setting, Brazil*

Characteristic Isolate†
ICBDVIM-2 ICBHVIM-2 ICBSVIM-2
Host/environment
 Dog
 Human
 Household environment
Sample
 Ear secretion, rectal swab, oral swab
 Feces
 Sofa swab
Isolation date
 2016 Dec 15; 2017 Mar 6
 2017 Mar 6
 2017 Mar 6
Resistance profile
 AMK, AMC, CAZ, CFO, CIP, CL, CPM, CRO, CTX, GEN, IMP, MER, NAL, PPT, STX, TET, TIC
 AMK, AMC, CAZ, CFO, CIP, CL, CPM, CRO, CTX, GEN, IMP, MER, NAL, PPT, STX, TET, TIC
 AMK, AMC, CAZ, CFO, CIP, CL, CPM, CRO, CTX, GEN, IMP, MER, NAL, PPT, STX, TET, TIC
Carbapenem MIC, μg/mL‡
 >32
 >32
 >32
Resistance genes to:
  β-Lactams blaVIM-2, blaPAO, blaOXA-4, blaOXA-50 blaVIM-2, blaPAO, blaOXA-4, blaOXA-50 blaVIM-2, blaPAO, blaOXA-4, blaOXA-50
  Aminoglycosides aadA2, aac(3)-Id, aph(3)-IIb aadA2, aac(3)-Id, aph(3)-IIb aadA2, aac(3)-Id, aph(3)-IIb
  Chloramphenicol catB7, cmlA1 catB7, cmlA1 catB7, cmlA1
  Sulfonamides sul1 sul1 sul1
  Trimethoprim dfrB5 dfrB5 dfrB5
  Tetracyclines tetG tetG tetG
  Fosfomycin
 fosA
 fosA
 fosA
Location of blaVIM-2
 Chromosome
 Chromosome
 Chromosome
MLST,(ST/CC 233/233 233/233 233/233

*AMK, amikacin; AMC, amoxicillin/clavulanic acid; CAZ, ceftazidime; CC, clonal complex; CFO, cefoxitin; CIP, ciprofloxacin; CL, chloramphenicol; CPM, cefepime; CRO, ceftriaxone; CTX, cefotaxime; GEN, gentamicin; IMP, imipenem; MER, meropenem; MLST, multilocus sequence typing; NAL, nalidixic acid; PPT, piperacillin/tazobactam; ST, sequence type; SXT, trimethoprim/sulfamethoxazole; TET, tetracycline, TIC, ticarcillin.
†Clonally related P. aeruginosa strains ICBDVIM-2 (ear secretion), ICBRVIM-2 (rectal swab), and ICBBVIM-2 (oral swab) were isolated from samples collected in the infected dog. ICBDVIM-2 was isolated on December 15, 2016. ICBRVIM-2 and ICBBVIM-2 were isolated on March 6, 2017. All P. aeruginosa strains from the infected dog displayed identical resistance profiles and genetic backgrounds.
‡Imipenem and meropenem.

Main Article

References
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1These authors contributed equally to this article.

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