Volume 24, Number 8—August 2018
Brucellosis in Dogs and Public Health Risk
|Test type||Antigen detected or target DNA||Sensitivity, %||Specificity, %||Reference|
|Rapid slide agglutination||Cell wall||50–75||83.34–99.7||(19)|
|2-mercaptoethanol rapid slide agglutination||Cell wall||31.76–70||100||(19)|
|Agar-gel immunodiffusion, cell wall antigen||LPS, outer membrane protein||27.98–52.94||100||(19)|
|ELISA||LPS or CPAg||88–97||94.3–96.7||(20)|
||R-LPS with outer membrane proteins
|PCR (ITS66 and ITS279)||16S-23S rRNA gene||100||86.45–100||(23)|
|PCR (JPF/JPR)||Outer membrane protein 2||16.67 (whole blood); 92.31 (vaginal swab sample)||100 (whole blood); 51.92 (vaginal swab sample)||(24)|
*CPAg, cytoplasmic protein antigen; JPF, forward primer; JPR, reverse primer; LPS, lipopolysaccharide; R-LPS, rough LPS.
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