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Volume 24, Number 8—August 2018


Brucellosis in Dogs and Public Health Risk

Martha E. HenselComments to Author , Maria Negron, and Angela M. Arenas-Gamboa
Author affiliations: Texas A&M University, College Station, Texas, USA (M.E. Hensel, A.M. Arenas-Gamboa); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M. Negron)

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Diagnostic tests for Brucella canis in dogs*

Test type Antigen detected or target DNA Sensitivity, % Specificity, % Reference
Rapid slide agglutination Cell wall 50–75 83.34–99.7 (19)
2-mercaptoethanol rapid slide 
agglutination Cell wall 31.76–70 100 (19)
Agar-gel immunodiffusion, cell wall 
antigen LPS, outer membrane protein 27.98–52.94 100 (19)
ELISA LPS or CPAg 88–97 94.3–96.7 (20)
R-LPS with outer membrane proteins
PCR (ITS66 and ITS279) 16S-23S rRNA gene 100 86.45–100 (23)
PCR (JPF/JPR) Outer membrane protein 2 16.67 (whole blood); 92.31 (vaginal swab sample) 100 (whole blood); 51.92 (vaginal swab sample) (24)

*CPAg, cytoplasmic protein antigen; JPF, forward primer; JPR, reverse primer; LPS, lipopolysaccharide; R-LPS, rough LPS.

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