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Volume 24, Number 8—August 2018

Brucellosis in Dogs and Public Health Risk

Martha E. HenselComments to Author , Maria Negron, and Angela M. Arenas-Gamboa
Author affiliations: Texas A&M University, College Station, Texas, USA (M.E. Hensel, A.M. Arenas-Gamboa); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M. Negron)

Main Article


Diagnostic tests for Brucella canis in dogs*

Test type Antigen detected or target DNA Sensitivity, % Specificity, % Reference
Rapid slide agglutination Cell wall 50–75 83.34–99.7 (19)
2-mercaptoethanol rapid slide 
agglutination Cell wall 31.76–70 100 (19)
Agar-gel immunodiffusion, cell wall 
antigen LPS, outer membrane protein 27.98–52.94 100 (19)
ELISA LPS or CPAg 88–97 94.3–96.7 (20)
R-LPS with outer membrane proteins
PCR (ITS66 and ITS279) 16S-23S rRNA gene 100 86.45–100 (23)
PCR (JPF/JPR) Outer membrane protein 2 16.67 (whole blood); 92.31 (vaginal swab sample) 100 (whole blood); 51.92 (vaginal swab sample) (24)

*CPAg, cytoplasmic protein antigen; JPF, forward primer; JPR, reverse primer; LPS, lipopolysaccharide; R-LPS, rough LPS.

Main Article

Page created: July 17, 2018
Page updated: July 17, 2018
Page reviewed: July 17, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.