Figure 3. Molecular phylogenetic analysis by maximum-likelihood method of isolates from a patient in Germany with recurrent fever episodes after traveling to southern Africa, 2015. A) Phylogeny of uvrA sequence fragments. The tree with the highest log likelihood (–2566.8936) is shown. A discrete gamma distribution was used to model evolutionary rate differences among sites (4 categories [+G, parameter = 0.9541]). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 43.5691% sites). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 19 nt sequences. There were a total of 570 positions in the final dataset. Bootstrap values >50 are shown. Black dot indicates the sample analyzed in this study. Black triangle represents the clade containing B. burgdorferi s.l. isolates, collapsed for simplicity. Scale bar indicates substitutions per site. B) Phylogeny of concatenated sequences of 7 MLST housekeeping loci (clpX, nifS, pepX, pyrG, recG, rplB, uvrA). The tree with the highest log likelihood (−31066.7852) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete gamma distribution was used to model evolutionary rate differences among sites (4 categories [+G, parameter = 0.7881]). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 36.6955% sites). The analysis involved 33 nt sequences. There were a total of 4,203 positions in the final dataset. The subtree containing the LB group of spirochetes was collapsed. Bootstrap values >50 are shown. Black dot indicates the sample analyzed in this study. Scale bar indicates substitutions per site.