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Volume 24, Number 9—September 2018
Dispatch

Correlation of Severity of Human Tick-Borne Encephalitis Virus Disease and Pathogenicity in Mice

Chaitanya Kurhade1, Sarah Schreier1, Yi-Ping Lee13, Loreen Zegenhagen4, Marika Hjertqvist, Gerhard Dobler, Andrea Kröger2, and Anna K. Överby2Comments to Author 
Author affiliations: Umeå University, Umeå, Sweden (C. Kurhade, Y.-P. Lee, A.K. Överby); Helmholtz Centre for Infection Research, Braunschweig, Germany (S. Schreier, L. Zegenhagen, A. Kröger); Otto-von-Guericke-University Magdeburg, Magdeburg, Germany (S. Schreier, A. Kröger); Public Health Agency of Sweden, Solna, Sweden (M. Hjertqvist); Bundeswehr Institute of Microbiology, Munich, Germany (G. Dobler); DZIF Partner Site Munich, Munich (G. Dobler)

Main Article

Figure 1

Survival analysis and TBEV burden in peripheral organs of Torö-2003–infected and HB171/11-infected C57BL/6 mice. A) Survival analysis of ten 6–10-week-old female C57BL/6 mice after subcutaneous inoculation with phosphate-buffered saline (mock, black) or with 104 focus forming units (FFU) of Torö-2003 (blue) or HB171/11 (red) in 100 μL phosphate-buffered saline. Survival differences were tested for statistical significance by log-rank test. B, C) Viral burdens in spleen (B) and lymph node (C) aft

Figure 1. Survival analysis and TBEV burden in peripheral organs of Torö-2003–infected and HB171/11-infected C57BL/6 mice. A) Survival analysis of ten 6–10-week-old female C57BL/6 mice after subcutaneous inoculation with phosphate-buffered saline (mock, black) or with 104 focus forming units (FFU) of Torö-2003 (blue) or HB171/11 (red) in 100 μL phosphate-buffered saline. Survival differences were tested for statistical significance by log-rank test. B, C) Viral burdens in spleen (B) and lymph node (C) after subcutaneous infection of Torö-2003 or HB171/11 (104 FFU, n = 5) were measured by quantitative PCR and normalized to intracellular glyceraldehyde 3-phosphate dehydrogenase (GAPDH) levels as previously described (14). Each data point represents an individual mouse. D–I) Cytokine response in spleen (D–F) and lymph node (G–I). Expression levels of GAPDH, TNF-α (D,G), IL-6 (E,H), and CXCL-10 (F,I) were determined by validated QuantiTect primer assays (QIAGEN, Hilden, Germany) and quantitative PCR from organs prepared in B and C. Signals of indicated mRNA were normalized to the GAPDH mRNA signal. Bars indicate mean values. Asterisks indicate statistical significance calculated by Mann-Whitney test (*p<0.05; **p<0.01). Horizontal black bars indicate mean values. a.u., arbitrary units; CXCL, CXC motif ligand; IL, interleukin; TBEV, tick-borne encephalitis virus; TNF, tumor necrosis factor.

Main Article

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1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

3Current affiliation: National Cheng Kung University, Tainan, Taiwan.

4Current affiliation: Charles River Laboratories, Freiburg, Germany.

Page created: August 15, 2018
Page updated: August 15, 2018
Page reviewed: August 15, 2018
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