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Volume 24, Number 9—September 2018
Research Letter

Spondweni Virus in Field-Caught Culex quinquefasciatus Mosquitoes, Haiti, 2016

Sarah K. White1Comments to Author , John A. Lednicky, Bernard A. Okech, J. Glenn Morris, and James C. Dunford
Author affiliations: University of Florida, Gainesville, Florida, USA (S.K. White, J.A. Lednicky, B.A. Okech, J.G. Morris, Jr.); US Navy and Marine Corps Public Health Center, Portsmouth, Virginia, USA (J.C. Dunford)

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Abstract

Spondweni virus (SPONV) and Zika virus cause similar diseases in humans. We detected SPONV outside of Africa from a pool of Culex mosquitoes collected in Haiti in 2016. This finding raises questions about the role of SPONV as a human pathogen in Haiti and other Caribbean countries.

Spondweni virus (SPONV) and Zika virus are closely related flaviviruses that were first described in Africa in 1952 and 1947, respectively (1). Humans infected by these viruses have similar clinical manifestations; asymptomatic infections are common, and illness is generally self-limiting (1). In the 6 documented human SPONV infections, fever occurred in all. Other symptoms included headache, nausea, myalgia, conjunctivitis, and arthralgia; only 1 SPONV-infected person had maculopapular and pruritic rash (1). The similar clinical presentations for these virus infections and reportedly high serologic cross-reactivity have resulted in frequent misdiagnosis (1).

Because of the 2015–2016 epidemic of Zika fever in the Western Hemisphere and the link between microcephaly and Zika virus infection, Zika virus has been studied more comprehensively than SPONV (1). SPONV was first isolated from Mansonia uniformis mosquitoes during virus surveillance in 1955 in South Africa (2). No new reports of SPONV surfaced despite continued mosquito surveillance until 1958, when it was identified in 4 additional mosquito species, including Aedes circumluteolus, a tropical sylvatic mosquito found in Africa (2). Little is known about possible vertebrate hosts, although SPONV antibodies have been detected in birds, small mammals, and ruminants (2). In a recent study by Haddow et al. strains of Ae. aegypti, Ae. albopictus, and Culex quinquefasciatus mosquitoes were not susceptible to SPONV infection (3).

We detected SPONV from a pool of 7 mixed-sex Cx. quinquefasciatus mosquitoes collected in July 2016 during ongoing arbovirus surveillance in Gressier, Haiti. During May–August 2016, we caught 1,756 mosquitoes using Biogents Sentinel traps (BioQuip Products, Rancho Dominguez, CA, USA) within a 10-mile radius in Gressier, a semirural setting. Trap locations were selected based on environmental considerations, low risk for traps being disturbed, and known human arbovirus-caused illnesses in the area (4). Trap bags were transported to a field laboratory in Haiti, where mosquitoes were frozen at –20°C, then identified by species and sexed by trained technicians using morphologic keys and identification guides (5,6). After identification, the mosquitoes were pooled by location, collection date, species (Ae. aegypti, Ae. albopictus, Cx. quinquefasciatus, and other), and sex. All pools were screened for chikungunya virus, dengue virus (DENV) serotypes 1–4, and Zika virus RNA by real-time reverse transcription PCR (rRT-PCR) (Technical Appendix Table 1), as we previously have done with human specimens from Haiti (4). Mosquito homogenates positive by rRT-PCR were used for sequencing using primer walking and Sanger sequencing methods as previously reported (4; Technical Appendix Table 2). In addition, we confirmed Aedes and Culex mosquito species by molecular methods (7,8). In initial screens of a pool of 7 mixed-sex Cx. quinquefasciatus mosquitoes (non–blood-fed) collected on July 4, 2016, rRT-PCR results suggested the presence of Zika virus RNA (cycle threshold value 39), but this same pool was negative for chikungunya virus and DENV RNA by rRT-PCR. After unsuccessful attempts to amplify Zika virus–specific amplicons using previously described Zika virus sequencing primers, we used an unbiased sequencing approach after treatment of virions in mosquito homogenate with cyanase (4). Because we suspected a closely related virus, we next tested random hexamers and SPONV-specific primers (Technical Appendix Table 3), which resulted in formation of virus-specific amplicons (Technical Appendix). Thereafter, using SPONV primers, we determined a 10,290-nt nearly complete genome and deposited it in GenBank (accession no. MG182017).

The SPONV genome from Haiti shared 10,174 (98.8%) of 10,290 nt identity with a SPONV isolate from mosquitoes in South Africa in 1954 (GenBank accession no. DQ859064) and 9,958 (96.8%) of 10,287 nt identity with the SPONV Chuku strain from blood of a febrile human patient in Nigeria in 1952 (accession no. KX227369) (Table). When compared with the Zika virus reference strain from Uganda (accession no. KY989511), a strain from Puerto Rico (accession no. KU501215), and a strain from Haiti in 2016 (accession no. MF384325), Zika virus and SPONV clearly continue to diverge because the nucleotide and amino acid identities of SPONV are less similar to more recent strains of Zika virus (Table). Few SPONV sequences have been deposited into GenBank, resulting in insufficient information to predict how and when SPONV was introduced in Haiti.

In the Americas and the Caribbean, SPONV is a potential emergent arbovirus and public health threat that manifests clinically with symptoms and signs similar to those of Zika virus infection (2,9). Misdiagnosis has been documented, and it is possible that SPONV has caused human infection in Haiti but has been misidentified as infection from DENV or other arboviruses (9). Little is known about SPONV pathogenesis, host range, and vector competency, especially with vectors present in the Western Hemisphere. Our detection of SPONV in Cx. quinquefasciatus mosquitoes raises questions about the role of this species as a vector for this virus and highlights the need for ongoing surveillance for SPONV infection among humans in the Caribbean, combined with studies of potential vector populations.

Dr. White is a senior scientist in Assay Development and Analytic, and Small Scale Development at Brammer Bio. During this study, she was a postdoctoral associate in the Department of Environmental and Global Health at the Emerging Pathogens Institute, University of Florida, under the mentorship of Dr. John Lednicky. Her primary research interests include emerging arboviruses, influenza D virus, and novel viral vector therapeutics.

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Acknowledgments

We thank the field technicians for setting traps and collecting and identifying mosquitoes in Haiti.

This work was funded in part by the Armed Forces Health Surveillance Branch, Global Emerging Infections Surveillance Section, Proposal Management Information System (PROMIS) ID P014517E2, and a grant from the National Institutes of Health to J.G.M. (R01 AI26357-01S1).

The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the US Government.  Dr. Dunford is a military service member; this work was prepared as part of his official duties. Title 17, U.S.C., §105 provides that copyright protection under this title is not available for any work of the US Government. Title 17, U.S.C., §101 defines a US Government work as a work prepared by a military service member or employee of the US Government as part of that person’s official duties.

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References

  1. Haddow  AD, Woodall  JP. Distinguishing between Zika and Spondweni viruses. Bull World Health Organ. 2016;94:711711A. DOIPubMed
  2. McIntosh  BM, Kokernot  RH, Paterson  HE, De Meillon  B. Isolation of Spondweni virus from four species of culicine mosquitoes and a report of two laboratory infections with the virus. S Afr Med J. 1961;35:64750.PubMed
  3. Haddow  AD, Nasar  F, Guzman  H, Ponlawat  A, Jarman  RG, Tesh  RB, et al. Genetic characterization of Spondweni and Zika viruses and susceptibility of geographically distinct strains of Aedes aegypti, Aedes albopictus and Culex quinquefasciatus (Diptera: Culicidae) to Spondweni virus. PLoS Negl Trop Dis. 2016;10:e0005083. DOIPubMed
  4. Lednicky  J, Beau De Rochars  VM, El Badry  M, Loeb  J, Telisma  T, Chavannes  S, et al. Zika virus outbreak in Haiti in 2014: molecular and clinical data. PLoS Negl Trop Dis. 2016;10:e0004687. DOIPubMed
  5. Rueda  LM. Pictoral keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue virus transmission. Zootaxa. 2004;589:3341. DOI
  6. Unit  WRB. Mosquito ID: Culex (Cux.) quinquefasciatus [cited 2017 May 1]. http://www.wrbu.org/mqID/mq_medspc/AD/CXqui_hab.html
  7. Smith  JL, Fonseca  DM. Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: culicidae). Am J Trop Med Hyg. 2004;70:33945.PubMed
  8. Das  B, Swain  S, Patra  A, Das  M, Tripathy  HK, Mohapatra  N, et al. Development and evaluation of a single-step multiplex PCR to differentiate the aquatic stages of morphologically similar Aedes (subgenus: Stegomyia) species. Trop Med Int Health. 2012;17:23543. DOIPubMed
  9. Wolfe  MS, Calisher  CH, McGuire  K. Spondweni virus infection in a foreign resident of Upper Volta. Lancet. 1982;2:13068. DOIPubMed

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Tables

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Cite This Article

DOI: 10.3201/eid2409.171957

Original Publication Date: 7/31/2018

1Current affiliation: Brammer Bio, Alachua, Florida, USA.

Table of Contents – Volume 24, Number 9—September 2018

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Sarah K. White, Brammer Bio, 13859 Progress Blvd, Alachua, FL 32615, USA

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Page created: August 16, 2018
Page updated: August 16, 2018
Page reviewed: August 16, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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