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Volume 25, Number 1—January 2019
Dispatch

Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins

Alexandra Rockstroh, Luisa Barzon, Widuranga Kumbukgolla, Hoang Xuan Su, Erley Lizarazo, Maria Fernanda Vincenti-Gonzalez, Adriana Tami, Alice M.M. Ornelas, Renato Santana Aguiar, Daniel Cadar, Jonas Schmidt-Chanasit, and Sebastian UlbertComments to Author 
Author affiliations: Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany (A. Rockstroh, S. Ulbert); University of Padova, Padova, Italy (L. Barzon); Rajarata University of Sri Lanka, Mihinthale, Sri Lanka (W. Kumbukgolla); Vietnam Military Medical University, Hanoi, Vietnam (H.X. Su); University Medical Center Groningen, Groningen, the Netherlands (E. Lizarazo, M.F. Vincenti-Gonzalez, A. Tami); Federal University of Rio de Janeiro, Rio de Janeiro, Brazil (A.M.M. Ornelas, R.S. Aguiar); Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (D. Cadar, J. Schmidt-Chanasit); German Centre for Infection Research, Hamburg (J. Schmidt-Chanasit)

Main Article

Figure 2

DENV IgM ELISA endpoint titers, by serotype, of paired patient serum samples acquired at 2–4 time points after symptom onset, 2013–2018. A–C) Serum samples from returning travelers with residence in Germany: DENV-1 positive (A, B); DENV-2 positive (C). D–F) Serum samples from residents of DENV-endemic country Sri Lanka: DENV-2 (D, E) positive; DENV-4 positive (F). Data lines indicate average titers; error bars indicate SDs. The antigens in the ELISA were Equad proteins (i.e., envelope protein fr

Figure 2. DENV IgM ELISA endpoint titers, by serotype, of paired patient serum samples acquired at 2–4 time points after symptom onset, 2013–2018. A–C) Serum samples from returning travelers with residence in Germany: DENV-1 positive (A, B); DENV-2 positive (C). D–F) Serum samples from residents of DENV-endemic country Sri Lanka: DENV-2 positive (D, E); DENV-4 positive (F). Data lines indicate average titers; error bars indicate SDs. The antigens in the ELISA were Equad proteins (i.e., envelope protein from each DENV serotype with 4 amino acid changes T76R, Q77E, W101R, and L107R). DENV, dengue virus.

Main Article

Page created: December 18, 2018
Page updated: December 18, 2018
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