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Volume 25, Number 4—April 2019

Prolonged Shedding of Zika Virus RNA in Vaginal Secretions, Nicaragua

Yaoska Reyes1, Natalie M. Bowman1Comments to Author , Sylvia Becker-Dreps, Edwing Centeno, Matthew H. Collins, Guei-Jiun Alice Liou, and Filemón Bucardo
Author affiliations: Universidad Nacional Autónoma de Nicaragua, León, Nicaragua (Y. Reyes, E. Centeno, F. Bucardo); University of North Carolina, Chapel Hill, North Carolina, USA (N.M. Bowman, S. Becker-Dreps, M.H. Collins, G.-J.A. Liou)

Main Article


Clinical characteristics and viral shedding in 5 women with acute Zika virus infection, Nicaragua, 2016–2017*

Characteristic A013 A018 A020 A024 A039
Age, y 54 18 25 19 25
Duration of symptoms at enrollment, d 1 3 4 6 5
Pregnant at enrollment No Yes Yes Yes Yes
Estimated gestational age at enrollment, mo NA 7 7 5 1
Timing of delivery, days after symptom onset NA 39 61 149 305
Hematocrit, %, reference range 36–45 36 32 35 37 40
Leukocytes, cells/mm3, reference range 5,000–10,000 4,230 5,410 3,510 17,430 8,050
Sexual contact in the last 3 mo† No Yes Yes Yes Yes
Previous infection with dengue virus† Yes No No No No
Previous infection with chikungunya virus† Yes Yes No No Yes
Fluid Zika-positive by qRT-PCR at baseline
Blood, urine
Blood, saliva
Vaginal fluid PCR results, Ct1/Ct2,‡ at approximate days after symptom onset
7 NS NS NS 0/0 0/0
14 NS 31/0 32/33 NS 0/0
21 NS 35/0 34/36 0/0 37/0
28 33/0 0/0 NS 0/0 NS
60 NS 34/36 NS 36/0 NS
90 NS 35/0 NS 0/0 NS
180 NS 37/0 0/0 NS NS

*Ct, cycle threshold; NA, not applicable; NS, no sample; qRT-PCR, quantitative reverse transcription PCR.
†By participant report.
‡Ct1 refers to pan-Zika primers; Ct2 refers to Asian-specific primers.

Main Article

1These authors contributed equally to this article.

Page created: March 18, 2019
Page updated: March 18, 2019
Page reviewed: March 18, 2019
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