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Volume 25, Number 5—May 2019

Dispatch

Diagnosis of Imported Monkeypox, Israel, 2018

Noam Erez1, Hagit Achdout1, Elad Milrot, Yuval Schwartz, Yonit Wiener-Well, Nir Paran, Boaz Politi, Hadas Tamir, Tomer Israely, Shay Weiss, Adi Beth-Din, Ohad Shifman, Ofir Israeli, Shmuel Yitzhaki, Shmuel C. Shapira, Sharon MelamedComments to Author , and Eli Schwartz
Author affiliations: Israel Institute for Biological Research, Ness-Ziona, Israel (N. Erez, A. Achdout, E. Milrot, N. Paran, B. Politi, H. Tamir, T. Israely, S. Weiss, A. Beth-Din, O. Shifman, O. Israeli, S. Yitzhaki, S.C. Shapira, S. Melamed); Shaare-Zedek Medical Center, Jerusalem, Israel (Y. Schwartz, Y. Wiener-Well); Tel Aviv University, Tel Aviv, Israel (E. Schwartz)

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Figure 2

Transmission electron microscopy and cell culture–based diagnosis of monkeypox in patient in Israel, 2018. Virus particles were detected in lesion samples as either virion aggregates (arrows) (A) or individual virions (B) with a typical brick shape. Infected Vero cells depicted typical cytopathic effect, exhibiting cell detachment and rounding. Scale bar in A indicates 0.2 μm; scale bar in B indicates 100 nm. C) Immunofluorescent staining of infected cells; original magnification ×10. D) DNA sta

Figure 2. Transmission electron microscopy and cell culture–based diagnosis of monkeypox in patient in Israel, 2018. Virus particles were detected in lesion samples as either virion aggregates (arrows) (A) or individual virions (B) with a typical brick shape. Infected Vero cells depicted typical cytopathic effect, exhibiting cell detachment and rounding. Scale bar in A indicates 0.2 μm; scale bar in B indicates 100 nm. C) Infected Vero cells depicting typical cytopathic effect: cell detachment and rounding. Original magnification ×10. D) Immunofluorescent staining of infected Vero cells: DNA (DAPI [4′,6-diamidino-2-phenylindole] stain, blue) and monkeypox virus (green); viral factories are evident (arrows). Original magnification ×25.

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1These authors contributed equally to this article.

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