Figure 2. Transmission electron microscopy and cell culture–based diagnosis of monkeypox in patient in Israel, 2018. Virus particles were detected in lesion samples as either virion aggregates (arrows) (A) or individual virions (B) with a typical brick shape. Infected Vero cells depicted typical cytopathic effect, exhibiting cell detachment and rounding. Scale bar in A indicates 0.2 μm; scale bar in B indicates 100 nm. C) Infected Vero cells depicting typical cytopathic effect: cell detachment and rounding. Original magnification ×10. D) Immunofluorescent staining of infected Vero cells: DNA (DAPI [4′,6-diamidino-2-phenylindole] stain, blue) and monkeypox virus (green); viral factories are evident (arrows). Original magnification ×25.