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Volume 25, Number 7—July 2019
Research

High-Complexity Plasmodium falciparum Infections, North Central Nigeria, 2015–2018

Bitrus YakubuComments to Author , Ishaya Yohanna Longdet, Horsfall Jen Tony, Dinchi Tyem Davou, and Emmanuel Obishakin
Author affiliations: National Veterinary Research Institute Vom, Jos, Nigeria (B. Yakubu, D.T. Davou, E. Obishakin); University of Jos, Jos (I.Y. Longdet, H.J. Tony)

Main Article

Table 1

Primer sequences used for PCRs to screen and genotype samples collected in study of genetic diversity of Plasmodium falciparum parasites, North Central Nigeria, 2015–2018*

PCR description Primer name or type: sequence, 5′→3′ Size, bp Reference
Nest-1
rPLU1: TCAAAGATTAAGCCATGCAAGTGA 620
(27)
rPLU5: CCTGTTGTTGCCTTAAACTCC
Nest-2, genus-specific PCR
rPLU3: TTTTTATAAGGATAACTACGGAAAAGCTGT 240
(27)
rPLU4: TACCCGTCATAGCCATGTTAGGCCAATACC
Plasmodium falciparum species–specific PCR
rFAL1: TTAAACTGGTTTGGGAAAACCAAATATATT 205
(27)
rFAL2: ACACAATGAACTCAATCATGACTACCCGTC
msp1, primary reaction
Forward: CTAGAAGCTTTAGAAGATGCAGTATTG Variable
(28)
Reverse: CTTAAATAGTATTCTAATTCAAGTGGATCA
K1
Forward: AAATGAAGAAGAAATTACTACAAAAGGTGC Variable
(28)
Reverse: GCTTGCATCAGCTGGAGGGCTTGCACCAGA
MAD20
Forward: AAATGAAGGAACAAGTGGAACAGCTGTTAC Variable
(28)
Reverse: ATCTGAAGGATTTGTACGTCTTGAATTACC
RO33
Forward: TAAAGGATGGAGCAAATACTCAAGTTGTTG Variable
(28)
Reverse: CATCTGAAGGATTTGCAGCACCTGGAGATC
msp2, primary reaction
Forward: ATGAAGGTAATTAAAACATTGTCTATTATA Variable
(28)
Reverse: CTTTGTTACCATCGGTACATTCTT
FC27
Forward: AATACTAAGAGTGTAGGTGCARATGCTCCA Variable
(28)
Reverse: TTTTATTTGGTGCATTGCCAGAACTTGAAC
IC/3D7
Forward: AGAAGTATGGCAGAAAGTAAKCCTYCTACT Variable
(28)
Reverse: GATTGTAATTCGGGGGATTCAGTTTGTTCG
glurp, primary reaction
Forward: TGAATTTGAAGATGTTCACACTGAAC Variable
(28)
Reverse: GTGGAATTGCTTTTTCTTCAACACTAA
glurp Forward: TGTTCACACTGAACAATTAGATTTAGATCA Variable (28)
Reverse: GTGGAATTGCTTTTTCTTCAACACTAA

*glurp, glutamate-rich protein; msp1, merozoite surface protein 1; msp2, merozoite surface protein 2.

Main Article

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Page updated: August 19, 2019
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