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Volume 26, Number 11—November 2020
Research

Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories

Alexandria E. Kesterson, John E. Craig, Lara J. Chuvala, and Henry S. HeineComments to Author 
Author affiliation: Author affiliation: University of Florida, Orlando, Florida, USA

Main Article

Table 3

Sterility of select agent samples after sterile filtration and methanol procedure*‡

Agent (reference) Positive serum Positive plasma Positive BAL Positive overnight culture Positive BAL cell pellet
Bacillus anthracis 0/3 0/3 0/3 0/3 NT
Yersinia pestis 0/3 0/3 0/3 0/3 NT
Burkholderia mallei 0/3 0/3 0/3 0/3 NT
Burkholderia pesudomallei 0/3 0/3 0/3 0/3 0/3
Francisella tularensis (14) 0/6 0/6 1/6† 1/6† NT

*BAL, bronchoalveolar lavage; NT, not tested.
†Negative result caused by contaminated tube cap.
‡Total success rate for filtration: 97%

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References
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  2. Centers for Disease Control and Prevention; Animal and Plant Health Inspection Service. Guidance on the Inactivation or Removal of Select Agents or Toxins for Future Use. 2018 [cited 2019 Aug 4]. https://www.selectagents.gov/irg-intro.html
  3. US Government Accountability Office. Actions Needed to Improve Management of DOD's Biosafety and Biosecurity Program. 2018 [cited 2019 Aug 4]. https://www.gao.gov/products/GAO-18-422
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Page updated: October 17, 2020
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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