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Volume 26, Number 11—November 2020
Research

Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories

Alexandria E. Kesterson, John E. Craig, Lara J. Chuvala, and Henry S. HeineComments to Author 
Author affiliation: Author affiliation: University of Florida, Orlando, Florida, USA

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Table 3

Sterility of select agent samples after sterile filtration and methanol procedure*‡

Agent (reference) Positive serum Positive plasma Positive BAL Positive overnight culture Positive BAL cell pellet
Bacillus anthracis 0/3 0/3 0/3 0/3 NT
Yersinia pestis 0/3 0/3 0/3 0/3 NT
Burkholderia mallei 0/3 0/3 0/3 0/3 NT
Burkholderia pesudomallei 0/3 0/3 0/3 0/3 0/3
Francisella tularensis (14) 0/6 0/6 1/6† 1/6† NT

*BAL, bronchoalveolar lavage; NT, not tested.
†Negative result caused by contaminated tube cap.
‡Total success rate for filtration: 97%

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References
  1. US Department of Health and Human Services. Biosafety in microbiological and biomedical laboratories, 5th ed. Washington (DC): The Department; 2009.
  2. Centers for Disease Control and Prevention; Animal and Plant Health Inspection Service. Guidance on the Inactivation or Removal of Select Agents or Toxins for Future Use. 2018 [cited 2019 Aug 4]. https://www.selectagents.gov/irg-intro.html
  3. US Government Accountability Office. Actions Needed to Improve Management of DOD's Biosafety and Biosecurity Program. 2018 [cited 2019 Aug 4]. https://www.gao.gov/products/GAO-18-422
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  12. Tan  GG, Liu  Y, Sivalingam  SP, Sim  S-H, Wang  D, Paucod  J-C, et al. Burkholderia pseudomallei aerosol infection results in differential inflammatory responses in BALB/c and C57Bl/6 mice. J Med Microbiol. 2008;57:508–15. DOIPubMed
  13. Heine  HS, Chuvala  L, Riggins  R, Cirz  R, Cass  R, Louie  A, et al. Natural history of Francisella tularensis in aerosol-challenged BALB/c mice. Antimicrob Agents Chemother. 2016;60:1834–40. DOIPubMed
  14. Dauphin  LA, Bowen  MD. A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations. J Microbiol Methods. 2009;76:212–4. DOIPubMed

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Page updated: October 17, 2020
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