Volume 26, Number 11—November 2020
Research
Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
Table 3
Sterility of select agent samples after sterile filtration and methanol procedure*‡
Agent (reference) | Positive serum | Positive plasma | Positive BAL | Positive overnight culture | Positive BAL cell pellet |
---|---|---|---|---|---|
Bacillus anthracis | 0/3 | 0/3 | 0/3 | 0/3 | NT |
Yersinia pestis | 0/3 | 0/3 | 0/3 | 0/3 | NT |
Burkholderia mallei | 0/3 | 0/3 | 0/3 | 0/3 | NT |
Burkholderia pesudomallei | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
Francisella tularensis (14) | 0/6 | 0/6 | 1/6†| 1/6†| NT |
*BAL, bronchoalveolar lavage; NT, not tested.
†Negative result caused by contaminated tube cap.
‡Total success rate for filtration: 97%
References
- US Department of Health and Human Services. Biosafety in microbiological and biomedical laboratories, 5th ed. Washington (DC): The Department; 2009.
- Centers for Disease Control and Prevention; Animal and Plant Health Inspection Service. Guidance on the Inactivation or Removal of Select Agents or Toxins for Future Use. 2018 [cited 2019 Aug 4]. https://www.selectagents.gov/irg-intro.html
- US Government Accountability Office. Actions Needed to Improve Management of DOD's Biosafety and Biosecurity Program. 2018 [cited 2019 Aug 4]. https://www.gao.gov/products/GAO-18-422
- Leighton TJ, Doi RH. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J Biol Chem. 1971;246:3189–95.PubMed
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