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Volume 26, Number 2—February 2020
Research

Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi

Lisa Becherer, Sascha Knauf, Michael Marks, Simone Lueert, Sieghard Frischmann, Nadine Borst, Felix von Stetten, Sibauk Bieb, Yaw Adu-Sarkodie, Kingsley Asiedu, Oriol Mitjà1, and Mohammed Bakheit1Comments to Author 
Author affiliations: University of Freiburg, Freiburg, Germany (L. Becherer, N. Borst, F. von Stetten); Georg-August-University of Goettingen, Goettingen, Germany (S. Knauf); German Primate Centre, Goettingen (S. Knauf, S. Lueert); London School of Hygiene & Tropical Medicine, London, UK (M. Marks); Hospital for Tropical Diseases, London (M. Marks); Mast Diagnostica GmbH, Reinfeld, Germany (S. Frischmann, M. Bakheit); Hahn-Schickard, Freiburg, Germany (N. Borst, F. von Stetten); Papua New Guinea Department of Health, Port Moresby, Papua New Guinea (S. Bieb); Kwame Nkrumah University of Science and Technology, Kumasi, Ghana (Y. Adu-Sarkod); World Health Organization, Geneva, Switzerland (K. Asiedu); Lihir Medical Centre-International SOS, Newcrest Mining, Lihir Island, Papua New Guinea (O. Mitjà); Fundacio Lluita contra la Sida-Hospital Universitari Germans Trias i Pujol, Badalona, Spain (O. Mitjà); Barcelona Institute for Global Health-Hospital Clinic-University of Barcelona, Barcelona, Spain (O. Mitjà)

Main Article

Table 1

Comparison of clinical performance of biplex loop-mediated isothermal amplification for detection of Treponema pallidum and Haemophilus ducreyi (TPHD-LAMP) against singleplex TaqMan quantitative PCR*

Characteristics Sample size Treponema pallidum Haemophilus ducreyi
Total samples, no. 293
No. positive 60 163
Sensitivity, % (95% CI) 84.7 (72.5–92.4) 91.6 (85.8–95.3)
Specificity, % (95% CI)
95.7 (92.0–97.8)
 84.8 (77.4–90.1)
Lesions containing a single pathogen† 195
No. positive 48 151
Sensitivity, % (95% CI) 92.5 (78.5–98.0) 94.1 (88.4–97.2)
Specificity, % (95% CI)
95.7 (92.0–97.8)
 84.8 (77.4–90.1)
Lesions containing both pathogens† 19
No. positive 12 12
Sensitivity, % (95% CI) 68.4 (43.5–86.4) 73.7 (48.6–89.9)
Specificity, % (95% CI)
NA
 NA
Samples from Lihir Island, no. 57
No. positive 21 13
Sensitivity, % (95% CI) 90.5 (68.2–98.3) 76.5 (50.0–92.2)
Specificity, % (95% CI)
94.4 (80.0–99.0)
 100.0 (89.1–100)
Samples from Karkar Island, no. 184
No. positive 33 119
Sensitivity, % (95% CI) 78.1 (59.6–90.1) 94.2 (87.5–97.7)
Specificity, % (95% CI)
94.7 (89.5–97.5)
 74.7 (63.4–83.5)
Samples from Ghana, no. 52
No. positive 6 31
Sensitivity, % (95% CI) 100.0 (51.7–100) 90.9 (75.5–97.6)
Specificity, % (95% CI) 100.0 (90.4–100) 94.7 (71.9–99.7)

*NA, not applicable.
†Determined by quantitative PCR.

Main Article

1These senior authors contributed equally to this article.

Page created: January 17, 2020
Page updated: January 17, 2020
Page reviewed: January 17, 2020
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