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Volume 26, Number 2—February 2020
Research Letter

Persistence of Crimean-Congo Hemorrhagic Fever Virus RNA

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Leholonolo Mathengtheng12, Dominique Goedhals2, Phillip A. Bester, Jacqueline Goedhals, and Felicity J. BurtComments to Author 
Author affiliations: National Health Laboratory Service, Bloemfontein, South Africa; University of the Free State, Bloemfontein

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Abstract

Crimean-Congo hemorrhagic fever virus (CCHFV) causes severe disease with fatalities. Awareness of potential sources of infection is important to reduce risk to healthcare workers and contacts. We detected CCHFV RNA in formalin-fixed, paraffin-embedded tissues from a spontaneous abortion that were submitted for histology 9 weeks after a suspected CCHFV infection in the mother.

Crimean-Congo hemorrhagic fever virus (CCHFV) has the potential to emerge in areas where competent vectors are present and become a major risk to public health. Because of an absence of registered vaccines or specific antiviral treatment, CCHFV is included as one of the diseases prioritized by the World Health Organization for research and development in public health emergency contexts. Nosocomial infections are one of its transmission routes; thus, awareness of possible sources of infection is important for reducing risk to healthcare workers and other contacts of infected persons.

We detected a case of CCHFV infection in South Africa during a retrospective study, conducted in 2014, of serum samples from patients with suspected tickbite fever and no diagnosis. We retrospectively screened 196 serum samples that were collected during 2008–2011 from acutely ill patients in Free State Province and submitted to the routine serology laboratory of the Department of Medical Microbiology, National Health Laboratory Service (Bloemfontein, South Africa) with suspected rickettsial infection. The University of the Free State Health Science Ethics Committee provided ethics approval to screen residual diagnostic samples (ethics approval no. ETOVS 118/06).

For this screening, RNA was extracted from human serum samples using the QIAamp Viral RNA Mini Kit (QIAGEN, https://www.qiagen.com), according to the manufacturer’s instructions. We used RNA as template in a nested reverse transcription PCR (RT-PCR) with CCHFV primers designated F2, R3 and F3, R2 (1,2). The primers target a 536-bp region (F2, R3) and a 260-bp region (F3, R2) of the small segment using a nested format.

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Thumbnail of Detection of Crimean-Congo hemorrhagic fever virus (CCFFV) from a retrospectively tested human serum sample that was among those collected during 2008–2011 from acutely ill patients with suspected rickettsial infection, South Africa. Phylogenetic tree was constructed using a 186-bp region of the CCHVF small gene encoding the nucleoprotein sequence. Nucleotide data was obtained in the study for samples designated VBD2/08S (serum-derived), VBD 2/08T (tissue-derived), and retrieved fro

Figure. Detection of Crimean-Congo hemorrhagic fever virus (CCHFV) from a retrospectively tested human serum sample that was among those collected during 2008–2011 from acutely ill patients with suspected rickettsial infection, South Africa....

CCHFV RNA was amplified from 1 of the 196 samples. Determination of partial nucleotide sequence of the 260-bp amplicon confirmed that CCHFV RNA was amplified with 100% nt homology to previously identified South Africa strains (Figure). Because the screening was performed retrospectively on residual samples, no clinical details were available; however, a heat-inactivated aliquot of the serum had a CCHFV IgM titer of 1:40 and IgG titer of 1:10 by immunofluorescent antibody assays (EUROIMMUN, https://www.euroimmun.com), suggesting an acute infection. Laboratory records indicated that, 9 weeks after the blood sample was collected and submitted, an endometrial curettage sample taken after a spontaneous abortion was sent for histologic examination. Therefore, we retrieved a formalin-fixed paraffin-embedded tissue section of this sample from the archives and tested it retrospectively for evidence of CCHFV RNA using nested RT-PCR. We amplified a short fragment of the gene encoding the CCHFV nucleoprotein and confirmed its identity using nucleotide sequencing (Figure).

Because the biopsy sample contained both endometrial and placental tissue, the sample did not enable cellular localization of viral RNA or antigen and thus did not provide evidence directly linking the viral RNA to fetal demise. Similarly, the absence of retrospective maternal serum samples collected at the time of the spontaneous abortion, or fresh biopsy material, did not enable investigation for viremia. However, detection of viral RNA in tissue biopsy collected 9 weeks after detection of viral RNA in a serum sample suggests persistence of CCHFV RNA.

Persistent viral infection in selected sites has been demonstrated for other RNA viruses. Zika virus RNA is normally detectable in serum samples 3–10 days after symptom onset. Investigations on persistence of Zika virus RNA in formalin-fixed, paraffin-embedded fetal tissue from pregnant mothers with confirmed infection identified a time frame of 119–238 (mean 163) days from maternal symptom onset to detection of RNA by RT-PCR in brains and 15–210 (mean 81) days in placentas (3). The detection of viral RNA in a curettage specimen does not enable distinction between maternal and fetal infection but nevertheless extends the period during which viral RNA is detectable.

Little is known about the clinical course of CCHFV infections in pregnant women. Spontaneous abortions were reported in 24 (58.5%) of 41 mothers with CCHFV infection (4). Similarly, viral hemorrhagic fevers, such as from Ebola and Lassa viruses, during pregnancy have been associated with spontaneous abortion (5,6). Antigenemia has been detected 2–11 days after illness onset, viral RNA has been detected 1–18 days after initial symptoms, and infectious virus has been isolated from samples collected 1–12 days after onset (7). Whether pregnancy influences the duration of viremia is not known.

Although our findings are subject to some limitations and it is not possible to make any assumptions with regard to association between virus infection and fetal demise or distinguish between maternal and fetal infection, our findings extend the period for detecting CCHFV RNA after infection and raises the the potential for nosocomial infections. In addition, because the serum sample we tested was initially submitted for suspected tickbite fever, healthcare workers should consider CCHFV as part of the differential diagnosis for tickbite fever (rickettsial infections) in local patients and travelers returning from CCHFV-endemic regions.

Dr. Mathengtheng was a medical scientist at the National Health Laboratory Service and University of the Free State in Bloemfontein, South Africa, at the time of his death in 2014. His primary research interest was the investigation of mosquitoborne and tickborne viruses in southern Africa.

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Acknowledgment

Funding was obtained from the South African Research Chairs Initiative (Vector Borne and Zoonotic Pathogens Research, grant 98346) of the Department of Science and Technology and National Research Foundation.

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References

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  2. Burt  FJ. Laboratory diagnosis of Crimean–Congo hemorrhagic fever virus infections. Future Virol. 2011;6:83141. DOI
  3. Bhatnagar  J, Rabeneck  DB, Martines  RB, Reagan-Steiner  S, Ermias  Y, Estetter  LB, et al. Zika virus RNA replication and persistence in brain and placental tissue. Emerg Infect Dis. 2017;23:40514. DOIPubMed
  4. Pshenichnaya  NY, Leblebicioglu  H, Bozkurt  I, Sannikova  IV, Abuova  GN, Zhuravlev  AS, et al. Crimean-Congo hemorrhagic fever in pregnancy: A systematic review and case series from Russia, Kazakhstan and Turkey. Int J Infect Dis. 2017;58:5864. DOIPubMed
  5. Price  ME, Fisher-Hoch  SP, Craven  RB, McCormick  JB. A prospective study of maternal and fetal outcome in acute Lassa fever infection during pregnancy. BMJ. 1988;297:5847. DOIPubMed
  6. Black  BO, Caluwaerts  S, Achar  J. Ebola viral disease and pregnancy. Obstet Med. 2015;8:10813. DOIPubMed
  7. Burt  FJ, Leman  PA, Smith  JF, Swanepoel  R. The use of a reverse transcription-polymerase chain reaction for the detection of viral nucleic acid in the diagnosis of Crimean-Congo haemorrhagic fever. J Virol Methods. 1998;70:12937. DOIPubMed

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Cite This Article

DOI: 10.3201/eid2602.191460

Original Publication Date: 1/8/2020

1Deceased.

2These authors contributed equally to this article.

Table of Contents – Volume 26, Number 2—February 2020

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Felicity J. Burt, National Health Laboratory Service, Division of Virology, PO Box 339 (G23) Bloemfontein 9300, South Africa

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Page created: January 20, 2020
Page updated: January 20, 2020
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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