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Volume 26, Number 4—April 2020
Dispatch

Detection of Zoonotic Bartonella Pathogens in Rabbit Fleas, Colorado, USA

Shingo Sato1, R. Jory Brinkerhoff1Comments to Author , Erin Hollis, Shunta Funada, Avery B. Shannon, and Soichi Maruyama
Author affiliations: Nihon University College of Bioresource Sciences, Fujisawa, Japan (S. Sato, S. Funada, S. Maruyama); University of Richmond, Richmond, Virginia, USA (R.J. Brinkerhoff, E. Hollis, A.B. Shannon); University of KwaZulu-Natal, Pietermaritzburg, South Africa (R.J. Brinkerhoff)

Main Article

Table 2

Bartonella sequence identities for ssrA amplicons amplified from Cediopsylla inaequalis and Euhoplopsyllus glacialis fleas collected from 5 desert cottontail rabbits (Sylvilagus audubonii) in Boulder County, Colorado, USA*

Host no. Flea nos. Flea species Closest Bartonella strains/sequence homologies/% (GenBank accession no.)
305 e1 E. glacialis B. vinsonii subsp. berkhoffii strain Winnie/98.8% (CP003124)
522 e6, e25, e26, e29 E. glacialis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
e12 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
e32 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
c1 C. inaequalis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
c9 C. inaequalis B. rochalimae strain BMGH (JN029797) 100%
633 e1, e2, e3, e4, e5, e8, e9, e10, e11 E. glacialis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
e12 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
e13 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
673 e1 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
674 e5 E. glacialis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
e6 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)

*All samples in the table tested positive for Bartonella DNA by ssrA real-time PCR (11), whereas no samples that were negative by conventional ssrA PCR tested positive by real-time PCR.

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References
  1. Hill  WA, Brown  JP. Zoonoses of rabbits and rodents. Vet Clin North Am Exot Anim Pract. 2011;14:519–31, vii. DOIPubMedGoogle Scholar
  2. Heller  R, Kubina  M, Mariet  P, Riegel  P, Delacour  G, Dehio  C, et al. Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits. Int J Syst Bacteriol. 1999;49:283–8. DOIPubMedGoogle Scholar
  3. Angelakis  E, Lepidi  H, Canel  A, Rispal  P, Perraudeau  F, Barre  I, et al. Human case of Bartonella alsatica lymphadenitis. Emerg Infect Dis. 2008;14:1951–3. DOIPubMedGoogle Scholar
  4. Raoult  D, Roblot  F, Rolain  JM, Besnier  JM, Loulergue  J, Bastides  F, et al. First isolation of Bartonella alsatica from a valve of a patient with endocarditis. J Clin Microbiol. 2006;44:278–9. DOIPubMedGoogle Scholar
  5. Jeanclaude  D, Godmer  P, Leveiller  D, Pouedras  P, Fournier  PE, Raoult  D, et al. Bartonella alsatica endocarditis in a French patient in close contact with rabbits. Clin Microbiol Infect. 2009;15(Suppl 2):110–1. DOIPubMedGoogle Scholar
  6. Puges  M, Ménard  A, Berard  X, Geneviève  M, Pinaquy  JB, Edouard  S, et al. An unexpected case of Bartonella alsatica prosthetic vascular graft infection. Infect Drug Resist. 2019;12:2453–6. DOIPubMedGoogle Scholar
  7. Kernif  T, Parola  P, Ricci  JC, Raoult  D, Rolain  JM. Molecular detection of Bartonella alsatica in rabbit fleas, France. Emerg Infect Dis. 2010;16:2013–4. DOIPubMedGoogle Scholar
  8. Márquez  FJ. Molecular detection of Bartonella alsatica in European wild rabbits (Oryctolagus cuniculus) in Andalusia (Spain). Vector Borne Zoonotic Dis. 2010;10:731–4. DOIPubMedGoogle Scholar
  9. Márquez  FJ. Detection of Bartonella alsatica in European wild rabbit and their fleas (Spilopsyllus cuniculi and Xenopsylla cunicularis) in Spain. Parasit Vectors. 2015;8:56. DOIPubMedGoogle Scholar
  10. Brinkerhoff  RJ. Mammal and flea occurrence in association with black-tailed prairie dog (Cynomys ludovicianus) colonies: implications for interspecific plague transmission [dissertation]. Boulder (CO): University of Colorado; 2008.
  11. Diaz  MH, Bai  Y, Malania  L, Winchell  JM, Kosoy  MY. Development of a novel genus-specific real-time PCR assay for detection and differentiation of Bartonella species and genotypes. J Clin Microbiol. 2012;50:1645–9. DOIPubMedGoogle Scholar
  12. Abdullah  S, Helps  C, Tasker  S, Newbury  H, Wall  R. Pathogens in fleas collected from cats and dogs: distribution and prevalence in the UK. Parasit Vectors. 2019;12:71.
  13. de Sousa  KCM, do Amaral  RB, Herrera  HM, Santos  FM, Macedo  GC, de Andrade Pinto  PCE, et al. Genetic diversity of Bartonella spp. in wild mammals and ectoparasites in Brazilian Pantanal. Microb Ecol. 2018;76:544–54. DOIPubMedGoogle Scholar
  14. Gundi  VA, Billeter  SA, Rood  MP, Kosoy  MY. Bartonella spp. in rats and zoonoses, Los Angeles, California, USA. Emerg Infect Dis. 2012;18:631–3. DOIPubMedGoogle Scholar
  15. Márquez  FJ, Millán  J, Rodríguez-Liébana  JJ, García-Egea  I, Muniain  MA. Detection and identification of Bartonella sp. in fleas from carnivorous mammals in Andalusia, Spain. Med Vet Entomol. 2009;23:393–8. DOIPubMedGoogle Scholar

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1These authors contributed equally to this article.

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