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Volume 26, Number 4—April 2020

Detection of Zoonotic Bartonella Pathogens in Rabbit Fleas, Colorado, USA

Shingo Sato1, R. Jory Brinkerhoff1Comments to Author , Erin Hollis, Shunta Funada, Avery B. Shannon, and Soichi Maruyama
Author affiliations: Nihon University College of Bioresource Sciences, Fujisawa, Japan (S. Sato, S. Funada, S. Maruyama); University of Richmond, Richmond, Virginia, USA (R.J. Brinkerhoff, E. Hollis, A.B. Shannon); University of KwaZulu-Natal, Pietermaritzburg, South Africa (R.J. Brinkerhoff)

Main Article

Table 2

Bartonella sequence identities for ssrA amplicons amplified from Cediopsylla inaequalis and Euhoplopsyllus glacialis fleas collected from 5 desert cottontail rabbits (Sylvilagus audubonii) in Boulder County, Colorado, USA*

Host no. Flea nos. Flea species Closest Bartonella strains/sequence homologies/% (GenBank accession no.)
305 e1 E. glacialis B. vinsonii subsp. berkhoffii strain Winnie/98.8% (CP003124)
522 e6, e25, e26, e29 E. glacialis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
e12 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
e32 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
c1 C. inaequalis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
c9 C. inaequalis B. rochalimae strain BMGH (JN029797) 100%
633 e1, e2, e3, e4, e5, e8, e9, e10, e11 E. glacialis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
e12 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
e13 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
673 e1 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)
674 e5 E. glacialis B. v. subsp. berkhoffii strain Winnie/98.8% (CP003124)
e6 E. glacialis B. alsatica strain IBS 382/96.9% (JN029776)

*All samples in the table tested positive for Bartonella DNA by ssrA real-time PCR (11), whereas no samples that were negative by conventional ssrA PCR tested positive by real-time PCR.

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1These authors contributed equally to this article.

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