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Volume 26, Number 4—April 2020
Research Letter

Tick-Borne Relapsing Fever Caused by Borrelia persica in Traveler to Central Asia, 2019

Veronika Muigg1Comments to Author , Helena M.B. Seth-Smith1, Daniel Goldenberger, Adrian Egli, Beatrice Nickel, Roland Dürig, Esther Kuenzli, Vladimira Hinic2, and Andreas Neumayr2
Author affiliations: Swiss Tropical and Public Health Institute, Basel, Switzerland; and University of Basel, Basel (V. Muigg, B. Nickel, E. Kuenzli, A. Neumayr); University Hospital Basel, Basel (H.M.B. Seth-Smith, D. Goldenberger, A. Egli, V. Hinic); University of Basel, Basel (H.M.B. Seth-Smith, A. Egli); Maihofpraxis, Luzern, Switzerland (R. Dürig)

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Abstract

We report a case of tick-borne relapsing fever caused by Borrelia persica in a traveler returning to Switzerland from central Asia. After the disease was diagnosed by blood smear microscopy, the causative Borrelia species was confirmed by shotgun metagenomics sequencing. PCR and sequencing techniques provide highly sensitive diagnostic tools superior to microscopy.

We report a case of tick-borne relapsing fever (TBRF) in a 21-year-old male tourist who returned from Kyrgyzstan in July 2019 after having traveled for 5 months to Mexico, Taiwan, and central Asia (Uzbekistan, Tajikistan, and Kyrgyzstan). While in Tajikistan, he experienced acute fever of 39.5°C, chills, and generalized aches on June 11, which lasted 3 days. He experienced identical episodes around June 17 and 25.

After returning to Switzerland, he sought care on June 28 from his general practitioner, who referred him to the regional hospital, where malaria test results were negative. After the patient experienced 2 more episodes of fever (July 2 and 14), the general practitioner referred him to a tropical medicine specialist on July 15. Anamnesis revealed that the patient had consumed unpasteurized milk and had been bitten by insects nightly while trekking in Tajikistan. Other than fever of 38.5°C and pain on palpation of the liver, physical examination revealed no pathologic findings. Abdominal sonography showed a borderline enlarged spleen but was otherwise unremarkable. Chest radiography indicated no abnormalities. Laboratory results are shown in the Appendix.

Figure

Thumbnail of Giemsa-stained thick (A) and thin (B) blood films, demonstrating extracellular spirochetes. Original magnifications ×1,000.

Figure. Giemsa-stained thick (A) and thin (B) blood films, demonstrating extracellular spirochetes. Original magnifications ×1,000.

Detection of spirochetes in blood films (Figure) confirmed the diagnosis of a relapsing fever borreliosis, already suspected from the classical presentation of recurrent fever episodes separated by asymptomatic intervals of ≈1 week. Shortly after starting doxycycline, the patient experienced a self-limiting crisis with chills and fever of 41°C, which we interpret as Jarisch-Herxheimer reaction. Subsequently, the patient’s condition rapidly improved.

To determine the Borrelia species, we performed 16S rRNA gene sequencing from the blood sample. Analysis of traces of capillary-sequenced amplified DNA after broad-range 16S rRNA gene PCR (660bp), performed by using RipSeqMixed (Pathogenomix, https://www.pathogenomix.com), could not differentiate between Borrelia recurrentis and B. persica within the 5′ end of the 16S rRNA gene. Therefore, we used a short-read shotgun metagenomic sequencing approach on DNA on an Illumina NextSeq500 platform (https://www.illumina.com).

Of the 7.8 million sequencing reads, 692 (0.009% of the sequence data) mapped (by CLC Genomics Workbench v.12.0.3 [QIAGEN, https://www.qiagen.com] with a length fraction of 0.8 and a similarity fraction of 0.95) to a derived database of Borrelia genomes comprising reference genomes of B. recurrentis (GenBank accession nos. CP000999–CP001000), B. persica (Assembly accession AYOT), B. duttonii (Assembly accession AZIT), B. hispanica (Assembly accession AYOU), and B. crocidurae (GenBank accession no. LN609267). The top hit was to B. persica, with 684 (98.8%) mapped reads, followed by B. duttoni with 6 reads and B. recurrentis with 2 reads. Across the B. persica reference genome, reads from the isolate in this case mapped across the whole genome, representing sections of 101 of the 245 assembly scaffolds. We submitted the Borrelia reads to the European Nucleotide Archive (https://www.ebi.ac.uk/ena) under project PRJEB35490. We did not submit the 16S RNA gene sequence to GenBank because of the low quality of the sequence (multiple undetermined nucleotides).

These results strongly suggest that B. persica was the infectious agent of TBRF. Pending microscopic confirmation, we ordered several serologic studies, including assays to detect antibodies against the Borrelia species that cause Lyme disease and against rickettsial pathogens (Appendix Table 1). Whether the mildly elevated serologic titer for spotted fever Rickettsia resulted from cross-reactivity or coinfection with a tick-borne Rickettsia remains unclear.

TBRF occurs in temperate and tropical countries and is caused by several species of Borrelia maintained in enzootic cycles in which small mammals serve as animal reservoirs and Ornithodoros soft ticks as vectors. Humans are accidental hosts (except for B. duttonii in Africa, which seems strictly limited to humans with no identified animal reservoir), usually exposed to tick bites when sleeping in rustic cabins or caves (1). The disease is characterized by recurrent fever episodes separated by afebrile periods and constitutional symptoms. Complications include meningoencephalitis and treatment-induced Jarisch-Herxheimer reaction. Diagnosis can be made by microscopic examination of blood smears collected during fever episodes or by molecular methods.

TBRF in international travelers is rare. The GeoSentinel Surveillance Network reported only 4 cases of relapsing fever among 24,920 returning febrile travelers during 1997–2006 (2), and we found only 40 other travel-related cases published since 1982 (Appendix Table 2). Most TBRF infections in travelers are caused by B. crocidurae and almost exclusively acquired in Senegal. Recently, a new species, Candidatus Borrelia kalaharica, was found in 2 travelers to southern Africa (3,4).

Reports on B. persica infections are few and largely restricted to Iran and Israel. Only 2 other cases of B. persica infections in travelers returning from Uzbekistan/Tajikistan have been reported (5,6). Considering the wide geographic distribution of the transmitting tick, Ornithodoros tholozani (India, Pakistan, Afghanistan, western China, Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, Uzbekistan, Iran, Iraq, Turkey, Cyprus, Syria, Jordan, Israel, Egypt, and Libya [7,8]), considerable underreporting and underrecognition is likely. Although apparently rare, central nervous system involvement and acute respiratory distress syndrome may complicate TBRF caused by B. persica (9).

For patients with periodic fever and supporting exposure risk, clinicians should consider a differential diagnosis of TBRF and carefully examine blood smears by microscopy. Increasingly available PCR and sequencing techniques provide highly sensitive diagnostic tools superior to microscopy.

Dr. Muigg is a clinician in the Department of Medicine at the Swiss Tropical and Public Health Institute, Basel, Switzerland. Her research interests include infectious diseases with a focus on neglected tropical diseases.

Dr. Seth-Smith is a bioinformatician in the Division of Clinical Bacteriology at the University Hospital, Basel. Her research interests include analyzing bacterial genomes to better understand pathogens, both evolutionarily and within a hospital context.

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References

  1. Talagrand-Reboul  E, Boyer  PH, Bergström  S, Vial  L, Boulanger  N. Relapsing fevers: neglected tick-borne diseases. Front Cell Infect Microbiol. 2018;8:98. DOIPubMed
  2. Wilson  ME, Weld  LH, Boggild  A, Keystone  JS, Kain  KC, von Sonnenburg  F, et al.; GeoSentinel Surveillance Network. Fever in returned travelers: results from the GeoSentinel Surveillance Network. Clin Infect Dis. 2007;44:15608. DOIPubMed
  3. Fingerle  V, Pritsch  M, Wächtler  M, Margos  G, Ruske  S, Jung  J, et al. “Candidatus Borrelia kalaharica” detected from a febrile traveller returning to Germany from vacation in southern Africa. PLoS Negl Trop Dis. 2016;10:e0004559. DOIPubMed
  4. Stete  K, Rieg  S, Margos  G, Häcker  G, Wagner  D, Kern  WV, et al. Case report and genetic sequence analysis of Candidatus Borrelia kalaharica, southern Africa. Emerg Infect Dis. 2018;24:165964. DOIPubMed
  5. Kutsuna  S, Kawabata  H, Kasahara  K, Takano  A, Mikasa  K. The first case of imported relapsing fever in Japan. Am J Trop Med Hyg. 2013;89:4601. DOIPubMed
  6. Colin de Verdiere  N, Hamane  S, Assous  MV, Sertour  N, Ferquel  E, Cornet  M. Tickborne relapsing fever caused by Borrelia persica, Uzbekistan and Tajikistan. Emerg Infect Dis. 2011;17:13257. DOIPubMed
  7. Assous  MV, Wilamowski  A. Relapsing fever borreliosis in Eurasia—forgotten, but certainly not gone! Clin Microbiol Infect. 2009;15:40714. DOIPubMed
  8. Parola  P, Raoult  D. Ticks and tickborne bacterial diseases in humans: an emerging infectious threat. Clin Infect Dis. 2001;32:897928. DOIPubMed
  9. Yossepowitch  O, Gottesman  T, Schwartz-Harari  O, Soroksky  A, Dan  M. Aseptic meningitis and adult respiratory distress syndrome caused by Borrelia persica. Infection. 2012;40:6957. DOIPubMed

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Cite This Article

DOI: 10.3201/eid2604.191771

Original Publication Date: March 05, 2020

1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

Table of Contents – Volume 26, Number 4—April 2020

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Veronika Muigg, Department of Medicine, Swiss Tropical and Public Health Institute, Socinstrasse 57, Basel 4051, Switzerland

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Page created: March 17, 2020
Page updated: March 17, 2020
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