Volume 26, Number 9—September 2020
Dispatch
Identification of Streptococcus suis Meningitis by Direct Triplex Real-Time PCR, Burkina Faso
Table 1
Sequences and concentrations of primers and probes used to detect Streptococcus suis, S. agalactiae, and S. pyogenes, Burkina Faso*
Target gene | Forward primer, 5¢ ® 3¢ | Reverse primer, 5¢ ® 3¢ | Probe, 5¢ ® 3¢ | Concentration of oligo per reaction, nM† |
---|---|---|---|---|
cfb (7), GBS | GGGAACAGATTATGAAAAACCG | AAGGCTTCTACACGACTACCAA | AGACTTCATTGCGTGCCAACCCTGAGAC 5¢-HEX; 3¢-BHQ1 | 200/200/200 |
fbpS (8), S. suis | TCCRATRCTGCTCTGCCATT | TGATAGTAGAAGTCCAGCARACT | AATAGCCC”T”GAAAAMCAGCCACWYTTTGARA 5¢-FAM; 3¢-SpC6; “T” = BHQ1 | 200/200/100 |
spy (9), GAS | GCACTCGCTACTATTTCTTACCTCAA | GTCACAATGTCTTGGAAACCAGTAAT | CCGCAAC”T”CATCAAGGATTTCGTTACCA 5¢-Cy-5; 3¢- SpC6; “T” = BHQ2 | 300/300/100 |
RNaseP (10) | AGATTTGGACCTGCGAGCG | GAGCGGCTGTCCCACAAGT | TTCTGACCTGAAGGCTCTGCGCG 5¢-FAM; 3¢-BHQ1 | 400/400/100 |
*GAS, group A Streptococcus (Streptococcus pyogenes); GBS, group A Streptococcus (S. agalactiae); RNaseP, human ribonuclease P.
†Concentration of forward primer/reverse primer/probe.
References
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CrossRef reports the first page should be "1", not "e45". (Ref. 1 "Goyette-Desjardins, Auger, Xu, Segura, Gottschalk, 2014")
eXtyles has not updated the authors because of a name mismatch. Please compare "Mai" with "Thi Hoang Mai", "Hoa" with "Thi Hoa", "Nga" with "Vu Thieu Nga", "Linh" with "Dieu Linh", "Chau" with "Thi Hong Chau", and "Sinh" with "Xuan Sinh". The CrossRef authors are Thi Hoang Mai N, Thi Hoa N, Vu Thieu Nga T, Dieu Linh L, Thi Hong Chau T, Xuan Sinh D, et al.. (Ref. 5 "Mai, Hoa, Nga, Linh, Chau, Sinh, et al., 2008")
eXtyles has not updated the article title because Medline's is slightly different and Title Case. The Medline article title is Nationwide Trends in Bacterial Meningitis before the Introduction of 13-Valent Pneumococcal Conjugate Vaccine-Burkina Faso, 2011-2013.. (Ref. 6 "Kambiré, Soeters, Ouédraogo-Traoré, Medah, Sangare, Yaméogo, et al., 2016")