Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 26, Number 9—September 2020
Research Letter

Typhus Group Rickettsiosis, Brazilian Amazon

Antonio H.H. Minervino, Marcelo B. Labruna, Salatiel R. Dias, Francisco B. Costa, Thiago F. Martins, Phablo N.S. da Silva, and Álvaro A. Faccini-MartínezComments to Author 
Author affiliations: Federal University of Western Pará, Santarém, Brazil (A.H.H. Minervino, S.R. Dias, P.N.S. da Silva); University of São Paulo, São Paulo, Brazil (M.B. Labruna, T.F. Martins); Universidade Estadual do Maranhão, São Luís, Brazil (F.B. Costa); Asociación Colombiana de Infectología, Bogotá, Colombia (Á.A. Faccini-Martínez)

Cite This Article

Abstract

Rickettsia rickettsii infection is the only rickettsiosis included in the list of reportable diseases in Brazil, where typhus group rickettsioses, mainly murine typhus, have been underreported. We report a case of typhus group rickettsiosis with unique ecologic particularities in a patient from the Brazilian Amazon, where, to our knowledge, rickettsioses have not been reported.

Typhus group rickettsioses are vectorborne infectious diseases that include murine typhus, caused by Rickettsia typhi, and epidemic typhus, caused by R. prowazekii (1). R. typhi is maintained in an enzootic cycle involving small mammals (e.g., Rattus spp. rats and Didelphis spp. opossums) and their ectoparasites, mainly fleas (2). R. typhi is usually transmitted to humans by contamination of the bite site, mucosal or skin abrasions with rickettsia-containing ectoparasite feces, or inhalation in contaminated dust (2). R. prowazekii is transmitted mainly by feces of human clothing lice (Pediculus humanus humanus) or a sylvatic cycle in the United States by contact with ectoparasites of flying squirrels (1).

In Brazil, there have been few reports of murine typhus, mostly >60 years ago and all from human-modified landscapes in southeastern or southern regions, far from the Amazon (3). To our knowledge, Brazil has had only 1 case of recrudescent, epidemic typhus (Brill-Zinsser disease) in a refugee from Europe (4). Currently, Brazilian spotted fever (R. rickettsii infection), a tickborne disease, is the only rickettsiosis included in the list of notifiable diseases in Brazil (3). We report a new case of typhus group rickettsiosis in a patient from the Brazilian Amazon.

Figure

Thumbnail of Study site in which typhus group rickettsiosis was detected in a 37-year-old man, Brazilian Amazon. Red dots indicate sites in STNF in which the patient worked during the day in the 2 weeks before disease onset. During this same period, he spend the night at his house in Porto Trombetas District, where he denied any rat infestation. Insets show location of STNF in Pará state and Brazil. STNF, Saracá-Taquera National Forest.

Figure. Study site in which typhus group rickettsiosis was detected in a 37-year-old man, Brazilian Amazon. Red dots indicate sites in STNF in which the patient worked during the day in the...

On August 27, 2019, a 37-year-old man was admitted to a hospital at Porto Trombetas District (Pará State, eastern Amazon region of Brazil) (Figure) because of 4 days of fever, chills, headache, malaise, and productive cough, associated with mild respiratory distress 72 hours after fever onset. His daily work consisted of outdoor herpetologic monitoring activities in Saracá-Taquera National Forest (35 km from Porto Trombetas) during the 2 weeks before disease onset. He reported that while in the forest, he removed an attached tick from his right thigh 10 days before disease onset.

Physical examination showed fever (temperature 38.3°C), tachycardia, tachypnea, O2 saturation 91%, bilateral inguinal lymphadenopathy, no rash, and a furuncle lesion at the site of the tick bite on the right thigh, and no inoculation eschar. Blood analysis showed leukocytosis (15,000 cells/mm3); neutrophilia (84%); standard platelet count (221,000 platelets/μL); and increased levels of alanine aminotransferase (50 U/L), aspartate aminotransferase (38 U/L), and C-reactive protein (20 mg/L). Chest radiograph showed bilateral interstitial pulmonary infiltrates. Presumptive diagnoses of Lyme borreliosis or Brazilian spotted fever was made and the patient was admitted.

Supplementary oxygen, ceftriaxone, chloramphenicol, and doxycycline were administered, and serologic tests for Borrelia burgdorferi and Rickettsia spp. were performed. Twenty-four hours later, the patient showed clinical stability, major improvement of respiratory status, and no fever. After 7 days of antimicrobial drug treatment, he was discharged with complete resolution of symptoms.

A serum sample collected on August 28 (5 days after disease onset) showed negative results by commercial ELISA for B. burgdorferi IgM and IgG. Results of indirect fluorescent antibody assays were nonreactive for R. conorii IgG but positive (titer 1:64) for R. typhi IgG.

To confirm a presumptive diagnosis of rickettsiosis, we collected a second serum sample 2 months later and showed by using an in-house indirect fluorescent antibody assays (5) negative results for IgG (titer <1:64) against 6 Rickettsia species: R. felis; R. bellii; and the spotted fever group agents R. rickettsii, R. parkeri, R. amblyommatis, and R. rhipicephali. This serum sample had a titer of 1:2,048 against R. typhi (Wilmington strain), confirming typhus group rickettsiosis.

There have been previous reports of typhus group rickettsiae in ticks from other areas (6,7). After we considered the tick bite history of the patient, during October 12–14, 2019, we went to areas in Saracá-Taquera National Forest that the patient visited and collected 170 ticks in 7 species. Attempts to detect rickettsial DNA in these ticks showed only R. amblyommatis in Amblyomma cajennense sensu stricto ticks (Appendix).

In a retrospective interview, the patient recalled 3 additional activities during the 2 weeks before disease onset: a daily rest in the forest for a few minutes after work, seeking work materials in a small mammal trap-storage at a facility within the natural forest, and 2 short visits to his mother’s house in the urban area of Santarém municipality (Pará State). He denied rat infestation in his own house at Porto Trombetas but reported previous rat infestations in his mother’s house.

Borreliosis and rickettsioses have not been confirmed in the Brazilian Amazon (8). The patient’s tick bite history before onset of symptoms led clinicians to presume these diagnoses and initiate appropriate antimicrobial drug treatment (e.g., doxycycline) (1), with a successful outcome. Because R. amblyommatis, a possibly nonpathogenic species (1), was the only agent detected in collected ticks, we consider that the furuncle lesion at the site of the tick bite was a pyogenic, localized, skin infection, and not rickettsia related.

Because of similar clinical manifestations and serologic cross-reactions between typhus group rickettsiae (1), we could not confirm the typhus group etiologic agent of this case-patient. We presume R. typhi as a probable infection because he had potential occupational exposures with rodents or other small mammals and their ectoparasites, rather than clothing louse infestation (1), in addition to an absence of neurologic symptoms, which are more common for epidemic typhus than for murine typhus (9,10). Conversely, the fact that the patient had spent most of his time in a forest environment could also implicate a sylvatic cycle of typhus group rickettsia in the Amazon forest.

Dr. Minervino is an associate professor at the Federal University of Western Pará, Santarém, Brazil. His research interests include epidemiology of tick-borne diseases.

Top

Acknowledgment

We thank Thiago Moreira and Osvaldo Nunes for providing assistance during tick sampling; Guilheme and Helder Batista for providing logistical support; and Carlos Pinheiro and all personnel from the Chico Mendes Institute for Biodiversity Conservation in Porto Trombetas for providing valuable assistance in expediting authorizations and logistic support in the field.

Top

References

  1. Fang  R, Blanton  LS, Walker  DH. Rickettsiae as emerging infectious agents. Clin Lab Med. 2017;37:383400. DOIPubMed
  2. Azad  AF. Epidemiology of murine typhus. Annu Rev Entomol. 1990;35:55369. DOIPubMed
  3. Silva  LJ, Papaiordanou  PM. Murine (endemic) typhus in Brazil: case report and review. Rev Inst Med Trop São Paulo. 2004;46:2835. DOIPubMed
  4. Meira  JA, Jamra  M, Lodovici  J. Brill’s disease: (epidemic typhus recrudescence) [in Portuguese]. Rev Hosp Clin Fac Med Sao Paulo. 1955;10:23746.
  5. Angerami  RN, Krawczak  FS, Nieri-Bastos  FA, Santos  F, Medorima  C, Ribeiro Resende  M, et al. First report of African tick-bite fever in a South American traveler. SAGE Open Med Case Rep. 2018;6:2050313X18775301.
  6. Medina-Sanchez  A, Bouyer  DH, Alcantara-Rodriguez  V, Mafra  C, Zavala-Castro  J, Whitworth  T, et al. Detection of a typhus group Rickettsia in Amblyomma ticks in the state of Nuevo Leon, Mexico. Ann N Y Acad Sci. 2005;1063:32732. DOIPubMed
  7. Dzul-Rosado  K, Lugo-Caballero  C, Tello-Martin  R, López-Avila  K, Zavala-Castro  J. Direct evidence of Rickettsia typhi infection in Rhipicephalus sanguineus ticks and their canine hosts. Open Vet J. 2017;7:1659. DOIPubMed
  8. Rodriguez-Morales  AJ, Bonilla-Aldana  DK, Idarraga-Bedoya  SE, Garcia-Bustos  JJ, Cardona-Ospina  JA, Faccini-Martínez  ÁA. Epidemiology of zoonotic tick-borne diseases in Latin America: Are we just seeing the tip of the iceberg? F1000 Res. 2018;7:1988. DOIPubMed
  9. Tsioutis  C, Zafeiri  M, Avramopoulos  A, Prousali  E, Miligkos  M, Karageorgos  SA. Clinical and laboratory characteristics, epidemiology, and outcomes of murine typhus: A systematic review. Acta Trop. 2017;166:1624. DOIPubMed
  10. Bechah  Y, Capo  C, Mege  JL, Raoult  D. Epidemic typhus. Lancet Infect Dis. 2008;8:41726. DOIPubMed

Top

Figure

Top

Cite This Article

DOI: 10.3201/eid2609.201305

Original Publication Date: August 05, 2020

Table of Contents – Volume 26, Number 9—September 2020

Comments

Please use the form below to submit correspondence to the authors or contact them at the following address:

Álvaro A. Faccini-Martínez, Asociación Colombiana de Infectología, Carrera 15 No.118-03, Bogotá, Colombia

Send To

character(s) remaining.

Comment submitted successfully, thank you for your feedback.

Top

Page created: May 07, 2020
Page updated: August 20, 2020
Page reviewed: August 20, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external