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Volume 26, Number 9—September 2020
Dispatch

Persistence of Severe Acute Respiratory Syndrome Coronavirus 2 in Aerosol Suspensions

Alyssa C. Fears, William B. Klimstra, Paul Duprex, Amy Hartman, Scott C. Weaver, Kenneth S. Plante, Divya Mirchandani, Jessica Ann Plante, Patricia V. Aguilar, Diana Fernández, Aysegul Nalca, Aysegul Totura, David Dyer, Brian Kearney, Matthew Lackemeyer, J. Kyle Bohannon, Reed Johnson, Robert F. Garry, Doug S. Reed1, and Chad J. Roy1Comments to Author 
Author affiliations: Tulane University School of Medicine, New Orleans, Louisiana, USA (A.C. Fears, R.F. Garry, C.J. Roy):; University of Pittsburgh, Pittsburgh, Pennsylvania, USA (W.B. Klimstra, P. Duprex, A. Hartman, D.S. Reed); University of Texas Medical Branch, Galveston, Texas, USA (S.C. Weaver, K.S. Plante, D. Mirchandani, J.A. Plante, P.V. Aguilar, D. Fernández); U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA (A. Nalca, A. Totura, D. Dyer, B. Kearney); National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Maryland, USA (M. Lackemeyer, J.K. Bohannon, R. Johnson)

Main Article

Figure 2

Decay curves of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in aerosol suspension. A) Aerosol concentration of infectious SARS-CoV-2 as measured by plaque assay found in impinger samples collected at 5 time points of increased aging in aerosol suspension. B) Corresponding aerosol concentration of SARS-CoV-2 in time-matched impinger samples as a function of viral genome copies as measured by reverse transcription quantitative PCR. Both time point virus estimates were graphed, and

Figure 2. Decay curves of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in aerosol suspension. A) Aerosol concentration of infectious SARS-CoV-2 as measured by plaque assay found in impinger samples collected at 5 time points of increased aging in aerosol suspension. B) Corresponding aerosol concentration of SARS-CoV-2 in time-matched impinger samples as a function of viral genome copies as measured by reverse transcription quantitative PCR. Both time point virus estimates were graphed, and nonlinear least-squares regression analysis single-order decay with no outlier detection was performed, resulting in a poor curve fit by either method of viral quantitation resulting from number and lack of iterative samples in this analysis.

Main Article

1These authors contributed equally to this article.

Page created: June 15, 2020
Page updated: June 22, 2020
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