Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 27, Number 1—January 2021
Research Letter

Novel 6-Month Treatment for Drug-Resistant Tuberculosis, United States

Connie A. HaleyComments to Author , Patricia Macias, Supriya Jasuja, Betsy A. Jones, Marie-Claire Rowlinson, Roshni Jaimon, Pennelyn Onderko, Elaine Darnall, Maria E. Gomez, Charles Peloquin, David Ashkin, and Neela D. Goswami
Author affiliations: University of Florida, Gainesville, Florida, USA (C.A. Haley, M.E. Gomez, D. Ashkin); Cook County Department of Public Health, Chicago, Illinois, USA (P. Macias, S. Jasuja, R. Jaimon, P. Onderko); Florida Department of Health, Jacksonville, Florida, USA (B.A. Jones, M.-C. Rowlinson, D. Ashkin); Illinois Department of Public Health, Springfield, Illinois, USA (E. Darnall); University of Florida College of Pharmacy, Gainesville (C. Peloquin); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (N.D. Goswami)

Cite This Article

Abstract

The US Food and Drug Administration approved a 6-month regimen of pretomanid, bedaquiline, and linezolid for extensively drug-resistant or multidrug-intolerant tuberculosis after a trial in South Africa demonstrated 90% effectiveness 6 months posttreatment. We report on a patient who completed the regimen using a lower linezolid dose.

A woman from eastern Europe in her late 40s undergoing treatment for stage III cervical adenocarcinoma was found to have a right upper lobe pulmonary nodule. Pathology from tissue biopsy demonstrated necrotizing granulomas and numerous acid-fast bacilli (AFB); the sample was PCR positive for Mycobacterium tuberculosis. Adjuvant chemotherapy was held, and the patient was referred to the local public health department. The patient provided informed written consent for publication of her case study.

On evaluation, the patient was asymptomatic without physical findings and reported no previous diagnosis or treatment of tuberculosis (TB) disease or latent TB infection. Results of serologic testing for HIV and viral hepatitis B and C were negative. She had a mild chronic anemia and transient transaminitis during chemotherapy (peak alanine aminotransferase 215 IU/L; aspartate aminotransferase 185 IU/L). Three sputum samples were negative by AFB smear and culture; 1 was tested by PCR and was M. tuberculosis negative.

Treatment with rifampin, isoniazid, pyrazinamide, ethambutol and pyridoxine was initiated. Lung biopsy cultures grew M. tuberculosis, and GeneXpert MTB/RIF assay (Cepheid, https://www.cepheid.com) detected an rpoB mutation indicating likely rifampin resistance. Rapid molecular detection of drug resistance and growth-based drug susceptibility testing performed by the Centers for Disease Control and Prevention (CDC) and Florida Bureau of Public Health Laboratories yielded concordant results. We detected resistance to isoniazid, rifampin, the fluoroquinolones (levofloxacin and moxifloxacin), and an injectable (kanamycin), confirming a diagnosis of extensively drug-resistant TB (XDR TB). Resistance was also detected for pyrazinamide but not for ethambutol, bedaquiline, or linezolid. The patient and her medical providers, in consultation with a CDC-funded TB Center of Excellence (COE, https://www.cdc.gov/tb/education/tb_coe), determined that her best treatment option was a 6-month all-oral regimen of bedaquiline, pretomanid, and linezolid (BPaL).

BPaL was approved by the US Food and Drug Administration (FDA) on August 14, 2019, based in part on results from the Nix-TB trial in South Africa, which included patients with XDR TB or multidrug-resistant (MDR) TB who failed or were intolerant of prior therapy (1). Pretomanid, the novel agent in the regimen, is a nitroimidazooxazine that blocks cell-wall production in actively replicating MTB organisms and acts as a respiratory poison and protein synthesis inhibitor to kill nonreplicating persister organisms (2). Bedaquiline is a diarylquinoline that inhibits mycobacterial adenosine triphosphate synthase in replicating and persister organisms, and linezolid is an oxazolidinone that also inhibits protein synthesis (3,4). The combined activity of BPaL enables cure in a far shorter period compared with currently recommended 18- to 24-month MDR TB regimens (5). In the Nix-TB trial, BPaL produced favorable outcomes in 98/109 (90%) patients at 6 months posttreatment (1); in addition, little preexisting resistance to bedaquiline, pretomanid, or linezolid has been reported (4,6).

Because pretomanid was not yet commercially available in the United States, the TB Alliance required an FDA-approved single-patient investigational new drug application and provided 6 months of pretomanid acquired internationally. A bridging regimen of bedaquiline, linezolid, moxifloxacin, cycloserine, clofazimine, and ethambutol was initiated for 2 weeks, then was narrowed to BPaL when pretomanid arrived.

For this patient, we initiated linezolid at 600 mg/d, given the toxicity of the Nix-TB dose of 1,200 mg/d, the patient’s paucibacillary disease, and TB COE’s experience with linezolid dosing (1,4,7,8). Therapeutic drug monitoring performed at the University of Florida Infectious Diseases Pharmacokinetic Laboratory (https://idpl.pharmacy.ufl.edu) was used to maintain a linezolid peak of 12–26 µg/mL and trough <2 µg/mL to reduce drug-induced toxicity (4,9).

The patient received outpatient BPaL treatment 7 days a week by directly observed therapy. We assessed liver, renal, hematologic, and neurologic function plus QTc intervals at baseline and every 2–4 weeks during treatment (Table 1). A few weeks into therapy, the patient’s linezolid level 18 hours postdose was measured at 7.62 µg/mL (serum trough level at 24 hours was likely lower but was still higher than expected) (Table 2). To reduce the trough while maintaining a peak serum level 4–16 times over her M. tuberculosis isolate’s linezolid MIC of 0.12 μg/mL, we extended the linezolid dosing interval to 600 mg every Monday, Wednesday, and Friday. A subsequent linezolid trough at 48 hours was calculated at <2 µg/mL. The patient completed 182 doses of BPaL over 26 weeks without treatment interruptions. Other than mild nausea that responded to pantoprazole, she had no adverse events or notable changes in laboratory values or electrocardiographs. Nine months after completion, the patient remained well; the state health department expects to closely monitor her for recurrent TB for 24 months after BPaL completion.

The patient, physicians, and public health staff involved reported high satisfaction with BPaL. Providers and TB programs in the United States considering this regimen for TB patients can seek guidance from CDC Division of Tuberculosis Elimination or their TB COE. Current trials using BPaL, such as ZeNix (https://www.tballiance.org/portfolio/trial/11883), are evaluating lower doses and shorter duration of linezolid compared with those of the Nix-TB trial. The 6-month, all oral, highly effective BPaL regimen is a notable advancement toward reducing global TB deaths (10).

Dr. Haley is a medical consultant for the Southeast National TB Center and an adjunct clinical professor in the Department of Medicine, Division of Infectious Diseases and Global Medicine, University of Florida.

Top

References

  1. Conradie  F, Diacon  AH, Ngubane  N, Howell  P, Everitt  D, Crook  AM, et al.; Nix-TB Trial Team. Treatment of highly drug-resistant pulmonary tuberculosis. N Engl J Med. 2020;382:893902. DOIPubMedGoogle Scholar
  2. Keam  SJ. Pretomanid: first approval. Drugs. 2019;79:1797803. DOIPubMedGoogle Scholar
  3. Lee  M, Lee  J, Carroll  MW, Choi  H, Min  S, Song  T, et al. Linezolid for treatment of chronic extensively drug-resistant tuberculosis. N Engl J Med. 2012;367:150818. DOIPubMedGoogle Scholar
  4. Brown  AN, Drusano  GL, Adams  JR, Rodriquez  JL, Jambunathan  K, Baluya  DL, et al. Preclinical evaluations to identify optimal linezolid regimens for tuberculosis therapy. MBio. 2015;6:e0174115. DOIPubMedGoogle Scholar
  5. Nahid  P, Mase  SR, Migliori  GB, Sotgiu  G, Bothamley  GH, Brozek  JL, et al. Treatment of drug-resistant tuberculosis. An official ATS/CDC/ERS/IDSA clinical practice guideline. Am J Respir Crit Care Med. 2019;200:e93142. DOIPubMedGoogle Scholar
  6. Xu  J, Wang  B, Hu  M, Huo  F, Guo  S, Jing  W, et al. Primary clofazimine and bedaquiline resistance among isolates from patients with multidrug-resistant tuberculosis. Antimicrob Agents Chemother. 2017;61:e00239-17. DOIPubMedGoogle Scholar
  7. Peloquin  C. The role of therapeutic drug monitoring in mycobacterial infections. Microbiol Spectr. 2017;5. DOIPubMedGoogle Scholar
  8. Heinrichs  MT, Drusano  GL, Brown  DL, Maynard  MS, Sy  SKB, Rand  KH, et al. Dose optimization of moxifloxacin and linezolid against tuberculosis using mathematical modeling and simulation. Int J Antimicrob Agents. 2019;53:27583. DOIPubMedGoogle Scholar
  9. Song  T, Lee  M, Jeon  HS, Park  Y, Dodd  LE, Dartois  V, et al. Linezolid trough concentrations correlate with mitochondrial toxicity-related adverse events in the treatment of chronic extensively drug-resistant tuberculosis. EBioMedicine. 2015;2:162733. DOIPubMedGoogle Scholar
  10. Harding  E. WHO global progress report on tuberculosis elimination. Lancet Respir Med. 2020;8:19. DOIPubMedGoogle Scholar

Top

Tables

Top

Cite This Article

DOI: 10.3201/eid2701.203766

Table of Contents – Volume 27, Number 1—January 2021

Comments

Please use the form below to submit correspondence to the authors or contact them at the following address:

Connie A. Haley, Southeastern National Tuberculosis Center, Box 103600, Gainesville, FL 32610-3600, USA

Send To

10000 character(s) remaining.

Top

Page created: October 28, 2020
Page updated: December 21, 2020
Page reviewed: December 21, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external