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Volume 27, Number 11—November 2021
Research Letter

Spontaneous Bacterial Peritonitis Caused by Bordetella hinzii

Grace C. Wang1, Miranda J. Wallace1, Gayathri Krishnan, Patrick D. Olson, Abigail L. Carlson2, Gautam Dantas3, and James M. Fleckenstein3Comments to Author 
Author affiliations: Saint Louis University School of Medicine, St. Louis, Missouri, USA (G.C. Wang); Washington University School of Medicine, St. Louis (M.J. Wallace, G. Krishnan, P.D. Olson, A.L. Carlson, G. Dantas, J.M. Fleckenstein); St. Louis Veterans Affairs Health Care System, St. Louis (A.L. Carlson, J.M. Fleckenstein)

Main Article

Figure

Comparative genomic analyses of Bordetella hinzii isolates from a patient in Missouri, USA, with type and nontype Bordetella assemblies. After core-genome alignment (58 total core genes), a neighbor-joining phylogenetic tree rooted with Achromobacter xylosoxidans as the outgroup demonstrates the isolates from this study cluster with other previously deposited B. hinzii genomes. Pairwise ANI was performed against type assemblies. The isolates in this study meet the ANI threshold (>0.96%) for species-level identity with B. hinzii type assembly GCF_900637615.1 (7). Isolates were recovered from peritoneal fluid cultures collected at day 1 and day 5 (P1 and P5, respectively; P2sub is a subculture of P1). Blood isolates were recovered from blood cultures collected on day 1 (B1.1 and B1.2; B1.1sub is a subculture of B1.1). As previously observed (8), the type genomes for B. pertussis, B. parapertussis, and B. bronchiseptica represent an instance of previously established, distinct species that exceed the species-level ANI threshold relative to each other. T indicates assemblies generated from type material. Type assemblies are numbered 1–12 on vertical axes as follows: 1, GCF_001457475.1; 2, GCF_000067205.1; 3, GCF_001676705.1; 4, GCF_001676725.1; 5, GCF_000306945.1; 6, GCF_001525545.2; 7, GCF_001598655.1; 8, GCF_900078335.1; 9, GCF_003350095.1; 10, GCF_900445775.1; 11, GCF_000657795.2; 12, GCF_900637615.1. ANI, average nucleotide identity.

Figure. Comparative genomic analyses of Bordetella hinzii isolates from a patient in Missouri, USA, with type and nontype Bordetella assemblies. After core-genome alignment (58 total core genes), a neighbor-joining phylogenetic tree rooted with Achromobacter xylosoxidans as the outgroup demonstrates the isolates from this study cluster with other previously deposited B. hinzii genomes. Pairwise ANI was performed against type assemblies. The isolates in this study meet the ANI threshold (>0.96%) for species-level identity with B. hinzii type assembly GCF_900637615.1 (7). Isolates were recovered from peritoneal fluid cultures collected at day 1 and day 5 (P1 and P5, respectively; P2sub is a subculture of P1). Blood isolates were recovered from blood cultures collected on day 1 (B1.1 and B1.2; B1.1sub is a subculture of B1.1). As previously observed (8), the type genomes for B. pertussis, B. parapertussis, and B. bronchiseptica represent an instance of previously established, distinct species that exceed the species-level ANI threshold relative to each other. T indicates assemblies generated from type material. Type assemblies are numbered 1–12 on vertical axes as follows: 1, GCF_001457475.1; 2, GCF_000067205.1; 3, GCF_001676705.1; 4, GCF_001676725.1; 5, GCF_000306945.1; 6, GCF_001525545.2; 7, GCF_001598655.1; 8, GCF_900078335.1; 9, GCF_003350095.1; 10, GCF_900445775.1; 11, GCF_000657795.2; 12, GCF_900637615.1. ANI, average nucleotide identity.

Main Article

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1These first authors contributed equally to this article.

2Current affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

3These authors contributed equally to this article as co–principal investigators.

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Page updated: October 19, 2021
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