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Volume 27, Number 12—December 2021
Dispatch

Heartland Virus Transmission, Suffolk County, New York, USA

Alan P. Dupuis1Comments to Author , Melissa A. Prusinski1, Collin O’Connor, Joseph G. Maffei, Kiet A. Ngo, Cheri A. Koetzner, Michael P. Santoriello, Christopher L. Romano, Guang Xu, Fumiko Ribbe, Scott R. Campbell, Stephen M. Rich, P. Bryon Backenson, Laura D. Kramer, and Alexander T. Ciota
Author affiliations: New York State Department of Health, Slingerlands, New York, USA (A.P. Dupuis II, J.G. Maffei, K.A. Ngo, C.A. Koetzner, L.D. Kramer, A.T. Ciota); New York State Department of Health, Albany, New York, USA (M.A. Prusinski, C. O’Connor, P.B. Backenson); Suffolk County Department of Health Services, Yaphank, New York, USA (M.P. Santoriello, C.L. Romano, S.R. Campbell); University of Massachusetts, Amherst, Massachusetts, USA (G. Xu, F. Ribbe, S.M. Rich); State University of New York at Albany School of Public Health, Albany (L.D. Kramer, A.T. Ciota)

Main Article

Figure 2

Phylogenetic relationship among Heartland virus isolates, Suffolk County, Long Island, New York, USA. Separate alignments of large segments (A), medium segments (B), small segments (C), and partial nonstructural sequences (D) were created with MAFFT in Geneious version 11.1.5 (https://www.geneious.com). Maximum-likelihood analyses were completed with RAxML (https://cme.h-its.org) using 1,000 bootstraps. Bootstrap values are indicated at each node. Phylogenetic trees for each segment were rooted to SFTSV strain HB154 (GenBank accession nos. JQ733560–62). Guerta virus strain DXM was included as an additional outgroup (GenBank accession nos. 328591–93). New York isolates from this study (red text), together with the 3 previously available full-genome sequences (MO 2009-P1 [patient 1, GenBank accession nos. JX005842, 4, 6]; MO 2009-P2 [patient 2, GenBank accession nos. X005843, 5, and 7]; and TN 2013 [TN, GenBank accession nos. J740146–8]), were included in these analyses (panels A, B, and C). Six additional partial sequences available for a 606-nt region of the nonstructural protein gene (GenBank accession nos. C466555, KC466560, KC466561, KC466562, KC466563, and MT052710) are indicated in an unrooted maximum-likelihood tree in panel D. Scale bars indicate nucleotide substitutions per site. GTV; Guerta virus; SFTSV, severe fever with thrombocytopenia syndrome virus.

Figure 2. Phylogenetic relationship among Heartland virus isolates, Suffolk County, Long Island, New York, USA. Separate alignments of large segments (A), medium segments (B), small segments (C), and partial nonstructural sequences (D) were created with MAFFT in Geneious version 11.1.5 (https://www.geneious.com). Maximum-likelihood analyses were completed with RAxML (https://cme.h-its.org) using 1,000 bootstraps. Bootstrap values are indicated at each node. Phylogenetic trees for each segment were rooted to SFTSV strain HB154 (GenBank accession nos. JQ733560–62). Guerta virus strain DXM was included as an additional outgroup (GenBank accession nos. 328591–93). New York isolates from this study (red text), together with the 3 previously available full-genome sequences (MO 2009-P1 [patient 1, GenBank accession nos. JX005842, 4, 6]; MO 2009-P2 [patient 2, GenBank accession nos. X005843, 5, and 7]; and TN 2013 [TN, GenBank accession nos. J740146–8]), were included in these analyses (panels A, B, and C). Six additional partial sequences available for a 606-nt region of the nonstructural protein gene (GenBank accession nos. C466555, KC466560, KC466561, KC466562, KC466563, and MT052710) are indicated in an unrooted maximum-likelihood tree in panel D. Scale bars indicate nucleotide substitutions per site. GTV; Guerta virus; SFTSV, severe fever with thrombocytopenia syndrome virus.

Main Article

1These authors contributed equally to this article.

Page created: August 16, 2021
Page updated: November 09, 2021
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