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Volume 27, Number 6—June 2021
Dispatch

Molecular Characterization and Antimicrobial Resistance in Neisseria gonorrhoeae, Nunavut Region of Inuit Nunangat, Canada, 2018–2019

Ameeta E. SinghComments to Author , Jasmine Pawa, Kethika Kulleperuma, Errol Prasad, Sonia Marchand, K. Dionne, Maxim Trubnikov, Tom Wong, Michael R. Mulvey, and Irene Martin
Author affiliations: University of Alberta, Edmonton, Alberta, Canada. (A.E. Singh); Government of Nunavut, Iqaluit, Nunavut, Canada (J. Pawa, K. Kulleperuma); DynaLIFE Medical Labs, Edmonton (E. Prasad); Qikiqtani General Hospital Laboratory, Iqaluit, Nunavut (S. Marchand, K. Dionne); Indigenous Services Canada, Ottawa, Ontario, Canada (M. Trubnikov, T. Wong); National Microbiology Laboratory, Winnipeg, Manitoba, Canada (M.R. Mulvey, I. Martin)

Main Article

Figure 2

Genetic relationship of Neisseria gonorrhoeae multiantigen sequence typing sequence types (STs) of gonorrhea-positive nucleic acid amplification specimens with prevalent and predicted nonsusceptible SNP assay results (n = 975) from a study of antimicrobial resistance in N. gonorrhoeae in the Nunavut region of Inuit Nunangat, Canada, 2018–2019. Only prevalent STs or STs whose samples predicted decreased susceptibility to cephalosporins or resistance to ciprofloxacin or azithromycin were included. The evolutionary history was inferred by using the maximum-likelihood method and Tamura-Nei model (13). The tree with the highest log likelihood (–4321.41) is shown. Initial trees for the heuristic search were obtained automatically by applying neighbor-joining and BioNJ algorithms to a matrix of pairwise distances estimated using the maximum composite likelihood approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 39 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding; a total of 917 positions were in the final dataset. Evolutionary analyses were conducted in MEGA X (14). Clusters were identified as STs with ≤5 base pair differences between them. *One of the ST2400 samples was predicted to be cephalosporin decreased susceptibility (not cephalosporin/DS). †The sample that was predicted to be cephalosporin/DS was not azithromycin resistant. ‡The sample that was cephalosporin/DS was also azithromycin resistant. Cephalosporin/DS, cephalosporin intermediate/decreased susceptibility.

Figure 2. Genetic relationship of Neisseria gonorrhoeae multiantigen sequence typing sequence types (STs) of gonorrhea-positive nucleic acid amplification specimens with prevalent and predicted nonsusceptible SNP assay results (n = 975) from a study of antimicrobial resistance in N. gonorrhoeae in the Nunavut region of Inuit Nunangat, Canada, 2018–2019. Only prevalent STs or STs whose samples predicted decreased susceptibility to cephalosporins or resistance to ciprofloxacin or azithromycin were included. The evolutionary history was inferred by using the maximum-likelihood method and Tamura-Nei model (13). The tree with the highest log likelihood (–4321.41) is shown. Initial trees for the heuristic search were obtained automatically by applying neighbor-joining and BioNJ algorithms to a matrix of pairwise distances estimated using the maximum composite likelihood approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 39 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding; a total of 917 positions were in the final dataset. Evolutionary analyses were conducted in MEGA X (14). Clusters were identified as STs with ≤5 base pair differences between them. *One of the ST2400 samples was predicted to be cephalosporin decreased susceptibility (not cephalosporin/DS). †The sample that was predicted to be cephalosporin/DS was not azithromycin resistant. ‡The sample that was cephalosporin/DS was also azithromycin resistant. Cephalosporin/DS, cephalosporin intermediate/decreased susceptibility.

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