Volume 27, Number 7—July 2021
Research
Multiplex Real-Time Reverse Transcription PCR for Influenza A Virus, Influenza B Virus, and Severe Acute Respiratory Syndrome Coronavirus 2
Table 2
Sensitivity of the Influenza SARS-CoV-2 Multiplex Assay compared with singleplex assays*
Viral titers† | Cycle threshold value‡ |
||||||
---|---|---|---|---|---|---|---|
Multiplex |
Singleplex |
||||||
A/Illinois/20/2018_(H1N1)pdm09 | |||||||
104.1 | 23.50 | 23.59 | 23.17 | 24.97 | 24.97 | 24.66 | |
103.4 | 26.57 | 26.50 | 26.81 | 27.71 | 27.49 | 27.50 | |
102.7 | 29.90 | 30.20 | 30.15 | 30.50 | 29.97 | 29.74 | |
102.0 | 35.58 | 35.24 | 36.17 | 32.32 | 32.43 | 33.63 | |
101.3 |
42.23 |
37.28 |
0 |
36.01 |
34.61 |
34.96 |
|
B/Colorado/06/2017_Victoria | |||||||
104.3 | 24.47 | 24.44 | 24.31 | 25.80 | 25.68 | 25.93 | |
103.6 | 27.57 | 27.45 | 27.71 | 28.84 | 28.98 | 29.30 | |
102.9 | 31.09 | 30.17 | 30.47 | 32.10 | 32.10 | 32.38 | |
102.2 | 34.38 | 33.49 | 34.43 | 35.19 | 35.49 | 35.99 | |
101.5 |
39.75 |
0 |
0 |
0 |
0 |
0 |
|
2019-nCoV/USA-WA1/2020 | |||||||
102.4 | 25.41 | 25.8 | 25.42 | 26.48 | 26.57 | 26.46 | |
101.7 | 28.69 | 28.87 | 28.5 | 30.26 | 29.77 | 29.51 | |
101.0 | 31.31 | 31.42 | 31.32 | 32.74 | 33.17 | 32.27 | |
100.3 | 35.14 | 36.36 | 34.58 | 36.10 | 35.34 | 35.81 | |
10–0.4 | 0 | 0 | 0 | 37.16 | 0 | 0 |
*Multiplex PCR is selective for influenza A virus, influenza B virus, and SARS-CoV-2. Boldface type indicates limits of detection. SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. †Viral titers are in relation to 50% tissue culture infectious dose, except for B/Colorado/06/2017, which is in relation to 50% egg infectious dose. ‡The multiplex cycle threshold values were derived from Influenza SARS-CoV-2 Multiplex Assay. The singleplex cycle threshold values were derived from the InfA (for influenza A), InfB (for influenza B), or N1 singleplex assays (for SARS-CoV-2). The N1 singleplex assay is a component of the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel (7). The InfA and InfB singleplex assays are components of the CDC Human Influenza Virus Real-Time RT-PCR Detection and Characterization Panel [510(k) no. K200370] (9). Each assay was performed in triplicate.
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1These first authors contributed equally to this article.
2These senior authors contributed equally to this article.