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Volume 28, Number 10—October 2022
Research Letter

Human Monkeypox without Viral Prodrome or Sexual Exposure, California, USA, 2022

Abraar KaranComments to Author , Ashley R. Styczynski, ChunHong Huang, Malaya K. Sahoo, Krithika Srinivasan, Benjamin A. Pinsky, and Jorge L. Salinas
Author affiliation: Stanford University School of Medicine, Stanford, California, USA

Main Article


Monkeypox virus DNA levels in clinical specimens from a man with monkeypox. California, USA, 2022*

Specimen type Days since symptom onset Viral copies/mL log10 copies/mL Ct value
Lesion swab 7 65,647,690 7.82 12.7
Nasopharyngeal swab 7 1,200 3.08 27.6
Saliva 10 3,030 3.48 26.3
Rectal swab 10 Detected, unable to quantitate NA 30.2
Conjunctival swab 10 Detected, unable to quantitate NA 32.6
Oropharyngeal swab 10 Not detected NA NA
Semen 10 Not detected NA NA

*The concentrations of the lesion, and self-collected rectal and conjunctival swab specimens are expressed in copies/mL phosphate-buffered saline (PBS). These samples, as well as the self-collected oropharyngeal swab, were collected dry and rehydrated with 1 mL PBS. The nasopharyngeal swab was healthcare worker–collected in 3 mL viral transport media. Cycle threshold values and estimated concentrations are based on the results from the non-variola orthopoxvirus quantitative PCR. All samples with values in copies/mL or reported as detected, unable to quantitate, were positive by both the non-variola orthopoxvirus qPCR and the clade 2/3 monkeypox qPCR. Ct, cycle threshold; NA, not applicable.

Main Article

Page created: August 11, 2022
Page updated: September 21, 2022
Page reviewed: September 21, 2022
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