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Volume 28, Number 12—December 2022
Dispatch

Isolation of Bat Sarbecoviruses, Japan

Shin MurakamiComments to Author , Tomoya Kitamura, Hiromichi Matsugo, Haruhiko Kamiki, Ken Oyabu, Wataru Sekine, Akiko Takenaka-Uema, Yuko Sakai-Tagawa, Yoshihiro Kawaoka, and Taisuke Horimoto
Author affiliations: The University of Tokyo, Tokyo, Japan (S. Murakami, H. Matsugo, H. Kamiki, W. Sekine, A. Takenaka-Uema, Y. Sakai-Tagawa, Y. Kawaoka, T. Horimoto); National Agriculture and Food Research Organization, Tokyo (T. Kitamura); Isumi County‒City Nature Preservation Association, Isumi-gun, Chiba, Japan (K. Oyabu)

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Figure 2

Growth kinetics of sarbecovirus isolates from bats in Japan. Rhinolophus cornutus bat isolates Rc-o319 (A), Rc-os20 (B), Rc-mk2 (C), and Rc-kw8 (D) or SARS-CoV-2 (B.1.1.7) € were inoculated into Vero/TMPRSS2 (WT), Vero-RcACE2 (RcACE2), Vero-hACE2 (hACE2), or Vero-ACE2KO (ACE2KO) cells at a multiplicity of infection of 0.01. The culture supernatants were collected at the indicated time points, and viral titers were determined by using a plaque assay. Data are reported as the mean titer with standard deviations from 3 independent experiments. ACE2, angiotensin converting enzyme 2; hACE2, human ACE2; RcACE2, R. cornutus ACE2; WT, wild-type.

Figure 2. Growth kinetics of sarbecovirus isolates from bats in Japan. Rhinolophus cornutus bat isolates Rc-o319 (A), Rc-os20 (B), Rc-mk2 (C), and Rc-kw8 (D) or SARS-CoV-2 (B.1.1.7) € were inoculated into Vero/TMPRSS2 (WT), Vero-RcACE2 (RcACE2), Vero-hACE2 (hACE2), or Vero-ACE2KO (ACE2KO) cells at a multiplicity of infection of 0.01. The culture supernatants were collected at the indicated time points, and viral titers were determined by using a plaque assay. Data are reported as the mean titer with standard deviations from 3 independent experiments. ACE2, angiotensin converting enzyme 2; hACE2, human ACE2; RcACE2, R. cornutus ACE2; WT, wild-type.

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